Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4321 and SO4322 are separated by 18 nucleotides SO4322 and SO4323 are separated by 8 nucleotides SO4323 and tRNA-Pro-3 are separated by 184 nucleotides tRNA-Pro-3 and tRNA-Pro-2 are separated by 53 nucleotides tRNA-Pro-2 and tRNA-His-2 are separated by 15 nucleotides tRNA-His-2 and tRNA-Arg-2 are separated by 52 nucleotides tRNA-Arg-2 and SO4324 are separated by 206 nucleotides
SO4321: SO4321 - OmpA family protein (NCBI ptt file), at 4,508,558 to 4,509,163
SO4321
SO4322: SO4322 - conserved hypothetical protein (NCBI ptt file), at 4,509,182 to 4,509,889
SO4322
SO4323: SO4323 - GGDEF domain protein (NCBI ptt file), at 4,509,898 to 4,511,817
SO4323
SO_t097: tRNA-Pro-3 - tRNA-Pro (RefSeq), at 4,512,002 to 4,512,078
tRNA-Pro-3
SO_t098: tRNA-Pro-2 - tRNA-Pro (RefSeq), at 4,512,132 to 4,512,208
tRNA-Pro-2
SO_t099: tRNA-His-2 - tRNA-His (RefSeq), at 4,512,224 to 4,512,299
tRNA-His-2
SO_t100: tRNA-Arg-2 - tRNA-Arg (RefSeq), at 4,512,352 to 4,512,428
tRNA-Arg-2
SO4324: SO4324 - GGDEF domain protein (NCBI ptt file), at 4,512,635 to 4,515,265
SO4324
Position (kb)
4509
4510
4511
4512 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4508.923 kb on - strand, within SO4321 at 4508.990 kb on + strand, within SO4321 at 4509.014 kb on + strand, within SO4321 at 4509.020 kb on + strand, within SO4321 at 4509.022 kb on - strand, within SO4321 at 4509.034 kb on - strand, within SO4321 at 4509.044 kb on - strand, within SO4321 at 4509.078 kb on + strand, within SO4321 at 4509.079 kb on + strand, within SO4321 at 4509.087 kb on - strand, within SO4321 at 4509.097 kb on + strand, within SO4321 at 4509.098 kb on - strand, within SO4321 at 4509.165 kb on + strand at 4509.262 kb on - strand, within SO4322 at 4509.264 kb on + strand, within SO4322 at 4509.344 kb on + strand, within SO4322 at 4509.363 kb on + strand, within SO4322 at 4509.481 kb on + strand, within SO4322 at 4509.489 kb on - strand, within SO4322 at 4509.489 kb on - strand, within SO4322 at 4509.558 kb on - strand, within SO4322 at 4509.562 kb on + strand, within SO4322 at 4509.637 kb on + strand, within SO4322 at 4509.766 kb on - strand, within SO4322 at 4509.836 kb on + strand at 4509.838 kb on - strand at 4509.843 kb on + strand at 4509.843 kb on + strand at 4509.843 kb on + strand at 4509.843 kb on + strand at 4509.844 kb on - strand at 4509.866 kb on - strand at 4509.866 kb on - strand at 4509.866 kb on - strand at 4509.896 kb on + strand at 4509.904 kb on - strand at 4509.939 kb on + strand at 4509.958 kb on + strand at 4509.959 kb on + strand at 4509.959 kb on + strand at 4509.966 kb on - strand at 4509.993 kb on - strand at 4510.018 kb on + strand at 4510.026 kb on - strand at 4510.026 kb on - strand at 4510.054 kb on - strand at 4510.098 kb on - strand, within SO4323 at 4510.265 kb on + strand, within SO4323 at 4510.274 kb on + strand, within SO4323 at 4510.502 kb on - strand, within SO4323 at 4510.761 kb on - strand, within SO4323 at 4510.886 kb on - strand, within SO4323 at 4510.912 kb on - strand, within SO4323 at 4510.932 kb on + strand, within SO4323 at 4510.973 kb on + strand, within SO4323 at 4510.975 kb on + strand, within SO4323 at 4511.131 kb on - strand, within SO4323 at 4511.242 kb on - strand, within SO4323 at 4511.267 kb on - strand, within SO4323 at 4511.278 kb on - strand, within SO4323 at 4511.537 kb on - strand, within SO4323 at 4511.537 kb on - strand, within SO4323 at 4511.597 kb on + strand, within SO4323 at 4511.597 kb on + strand, within SO4323 at 4511.602 kb on + strand, within SO4323 at 4511.612 kb on + strand, within SO4323 at 4511.644 kb on + strand at 4511.678 kb on - strand at 4511.727 kb on - strand at 4511.771 kb on - strand at 4511.863 kb on - strand at 4511.910 kb on + strand at 4512.089 kb on + strand at 4512.146 kb on - strand, within tRNA-Pro-2 at 4512.606 kb on + strand at 4512.611 kb on + strand at 4512.619 kb on - strand at 4512.630 kb on + strand at 4512.790 kb on + strand at 4512.790 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 4,508,923 - SO4321 0.60 +0.3 4,508,990 + SO4321 0.71 -0.1 4,509,014 + SO4321 0.75 +0.8 4,509,020 + SO4321 0.76 -0.2 4,509,022 - SO4321 0.77 +0.2 4,509,034 - SO4321 0.79 +0.1 4,509,044 - SO4321 0.80 -1.3 4,509,078 + SO4321 0.86 -0.3 4,509,079 + SO4321 0.86 +0.7 4,509,087 - SO4321 0.87 -0.7 4,509,097 + SO4321 0.89 +0.3 4,509,098 - SO4321 0.89 -0.7 4,509,165 + +1.2 4,509,262 - SO4322 0.11 -0.5 4,509,264 + SO4322 0.12 -0.2 4,509,344 + SO4322 0.23 -1.5 4,509,363 + SO4322 0.26 -1.2 4,509,481 + SO4322 0.42 -1.9 4,509,489 - SO4322 0.43 -1.1 4,509,489 - SO4322 0.43 -1.7 4,509,558 - SO4322 0.53 -0.3 4,509,562 + SO4322 0.54 -2.1 4,509,637 + SO4322 0.64 -1.5 4,509,766 - SO4322 0.82 -1.3 4,509,836 + +0.6 4,509,838 - +0.1 4,509,843 + +0.9 4,509,843 + -0.3 4,509,843 + -0.0 4,509,843 + +1.7 4,509,844 - -0.8 4,509,866 - -1.1 4,509,866 - -2.6 4,509,866 - -0.1 4,509,896 + +0.6 4,509,904 - -3.2 4,509,939 + -1.6 4,509,958 + +0.8 4,509,959 + -2.5 4,509,959 + -1.5 4,509,966 - -1.1 4,509,993 - -1.6 4,510,018 + -1.6 4,510,026 - -0.8 4,510,026 - -1.3 4,510,054 - -1.3 4,510,098 - SO4323 0.10 -2.8 4,510,265 + SO4323 0.19 -1.2 4,510,274 + SO4323 0.20 -1.4 4,510,502 - SO4323 0.31 -1.1 4,510,761 - SO4323 0.45 -1.5 4,510,886 - SO4323 0.51 -1.4 4,510,912 - SO4323 0.53 -1.9 4,510,932 + SO4323 0.54 -1.2 4,510,973 + SO4323 0.56 -0.5 4,510,975 + SO4323 0.56 -0.7 4,511,131 - SO4323 0.64 -0.7 4,511,242 - SO4323 0.70 -1.3 4,511,267 - SO4323 0.71 +0.2 4,511,278 - SO4323 0.72 -0.9 4,511,537 - SO4323 0.85 -1.1 4,511,537 - SO4323 0.85 -0.2 4,511,597 + SO4323 0.88 -0.8 4,511,597 + SO4323 0.88 +0.5 4,511,602 + SO4323 0.89 -0.3 4,511,612 + SO4323 0.89 -0.9 4,511,644 + -0.0 4,511,678 - -0.3 4,511,727 - -1.0 4,511,771 - +1.4 4,511,863 - +0.4 4,511,910 + -0.3 4,512,089 + +1.3 4,512,146 - tRNA-Pro-2 SO_t098 0.18 -2.8 4,512,606 + -0.2 4,512,611 + +0.3 4,512,619 - +0.2 4,512,630 + +0.2 4,512,790 + -0.0 4,512,790 + +0.2
Or see this region's nucleotide sequence