Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt vacB and motX are separated by 40 nucleotides motX and purA are separated by 143 nucleotides purA and SO3938 are separated by 66 nucleotides SO3938 and rpsI are separated by 113 nucleotides rpsI and rplM are separated by 14 nucleotides
SO3935: vacB - ribonuclease R (NCBI ptt file), at 4,079,522 to 4,081,948
vacB
SO3936: motX - sodium-type flagellar protein MotX (NCBI ptt file), at 4,081,989 to 4,082,609
motX
SO3937: purA - adenylosuccinate synthetase (NCBI ptt file), at 4,082,753 to 4,084,048
purA
SO3938: SO3938 - conserved hypothetical protein (NCBI ptt file), at 4,084,115 to 4,084,303
SO3938
SO3939: rpsI - ribosomal protein S9 (NCBI ptt file), at 4,084,417 to 4,084,809
rpsI
SO3940: rplM - ribosomal protein L13 (NCBI ptt file), at 4,084,824 to 4,085,252
rplM
Position (kb)
4082
4083
4084
4085 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4081.789 kb on - strand at 4081.814 kb on + strand at 4082.011 kb on + strand at 4082.050 kb on + strand at 4082.058 kb on - strand, within motX at 4082.121 kb on + strand, within motX at 4082.121 kb on + strand, within motX at 4082.142 kb on + strand, within motX at 4082.149 kb on - strand, within motX at 4082.151 kb on + strand, within motX at 4082.151 kb on + strand, within motX at 4082.159 kb on - strand, within motX at 4082.308 kb on - strand, within motX at 4082.313 kb on - strand, within motX at 4082.318 kb on - strand, within motX at 4082.331 kb on + strand, within motX at 4082.337 kb on + strand, within motX at 4082.505 kb on - strand, within motX at 4082.560 kb on - strand at 4082.572 kb on + strand at 4082.599 kb on + strand at 4082.791 kb on + strand at 4082.792 kb on + strand at 4082.937 kb on + strand, within purA at 4082.947 kb on + strand, within purA at 4083.046 kb on + strand, within purA at 4083.070 kb on + strand, within purA at 4083.088 kb on + strand, within purA at 4083.088 kb on + strand, within purA at 4083.091 kb on - strand, within purA at 4083.237 kb on + strand, within purA at 4083.237 kb on + strand, within purA at 4083.237 kb on + strand, within purA at 4083.245 kb on - strand, within purA at 4083.245 kb on - strand, within purA at 4083.245 kb on - strand, within purA at 4083.251 kb on - strand, within purA at 4083.338 kb on - strand, within purA at 4083.348 kb on - strand, within purA at 4083.359 kb on + strand, within purA at 4083.379 kb on - strand, within purA at 4083.470 kb on + strand, within purA at 4083.470 kb on + strand, within purA at 4083.585 kb on + strand, within purA at 4083.585 kb on + strand, within purA at 4083.704 kb on + strand, within purA at 4083.712 kb on - strand, within purA at 4083.712 kb on - strand, within purA at 4083.784 kb on + strand, within purA at 4083.850 kb on + strand, within purA at 4083.884 kb on - strand, within purA at 4083.920 kb on + strand at 4083.928 kb on - strand at 4083.928 kb on - strand at 4083.998 kb on - strand at 4084.113 kb on - strand at 4084.141 kb on + strand, within SO3938 at 4084.141 kb on + strand, within SO3938 at 4084.243 kb on + strand, within SO3938 at 4084.243 kb on + strand, within SO3938 at 4084.243 kb on + strand, within SO3938 at 4084.243 kb on + strand, within SO3938 at 4084.243 kb on + strand, within SO3938 at 4084.251 kb on - strand, within SO3938 at 4084.251 kb on - strand, within SO3938 at 4084.251 kb on - strand, within SO3938 at 4084.251 kb on - strand, within SO3938
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 4,081,789 - -0.7 4,081,814 + -0.3 4,082,011 + -2.3 4,082,050 + -0.3 4,082,058 - motX SO3936 0.11 +1.1 4,082,121 + motX SO3936 0.21 -0.5 4,082,121 + motX SO3936 0.21 +0.3 4,082,142 + motX SO3936 0.25 -0.3 4,082,149 - motX SO3936 0.26 +1.2 4,082,151 + motX SO3936 0.26 -0.1 4,082,151 + motX SO3936 0.26 +0.6 4,082,159 - motX SO3936 0.27 +2.1 4,082,308 - motX SO3936 0.51 +2.9 4,082,313 - motX SO3936 0.52 +2.0 4,082,318 - motX SO3936 0.53 +1.5 4,082,331 + motX SO3936 0.55 +2.1 4,082,337 + motX SO3936 0.56 +0.6 4,082,505 - motX SO3936 0.83 +2.7 4,082,560 - +1.5 4,082,572 + +1.0 4,082,599 + +1.6 4,082,791 + -1.5 4,082,792 + +1.7 4,082,937 + purA SO3937 0.14 -1.0 4,082,947 + purA SO3937 0.15 +0.0 4,083,046 + purA SO3937 0.23 -2.9 4,083,070 + purA SO3937 0.24 -0.1 4,083,088 + purA SO3937 0.26 +0.1 4,083,088 + purA SO3937 0.26 -0.5 4,083,091 - purA SO3937 0.26 -0.3 4,083,237 + purA SO3937 0.37 -0.1 4,083,237 + purA SO3937 0.37 -0.3 4,083,237 + purA SO3937 0.37 -0.6 4,083,245 - purA SO3937 0.38 -0.8 4,083,245 - purA SO3937 0.38 -0.4 4,083,245 - purA SO3937 0.38 -3.0 4,083,251 - purA SO3937 0.38 -0.3 4,083,338 - purA SO3937 0.45 -0.3 4,083,348 - purA SO3937 0.46 -0.2 4,083,359 + purA SO3937 0.47 -0.2 4,083,379 - purA SO3937 0.48 -0.9 4,083,470 + purA SO3937 0.55 -1.3 4,083,470 + purA SO3937 0.55 -1.9 4,083,585 + purA SO3937 0.64 -0.3 4,083,585 + purA SO3937 0.64 -0.5 4,083,704 + purA SO3937 0.73 -0.2 4,083,712 - purA SO3937 0.74 -1.4 4,083,712 - purA SO3937 0.74 -1.1 4,083,784 + purA SO3937 0.80 +0.2 4,083,850 + purA SO3937 0.85 +2.2 4,083,884 - purA SO3937 0.87 -2.3 4,083,920 + -0.2 4,083,928 - -0.8 4,083,928 - -0.2 4,083,998 - -0.5 4,084,113 - -0.4 4,084,141 + SO3938 0.14 +0.0 4,084,141 + SO3938 0.14 +0.9 4,084,243 + SO3938 0.68 +0.6 4,084,243 + SO3938 0.68 +0.0 4,084,243 + SO3938 0.68 -0.2 4,084,243 + SO3938 0.68 -0.8 4,084,243 + SO3938 0.68 +0.0 4,084,251 - SO3938 0.72 +0.1 4,084,251 - SO3938 0.72 -0.3 4,084,251 - SO3938 0.72 -0.5 4,084,251 - SO3938 0.72 +0.3
Or see this region's nucleotide sequence