Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysN and cysD are separated by 187 nucleotides cysD and cobA are separated by 80 nucleotides
SO3726: cysN - sulfate adenylyltransferase, subunit 1 (NCBI ptt file), at 3,869,200 to 3,870,627
cysN
SO3727: cysD - sulfate adenylyltransferase, subunit 2 (NCBI ptt file), at 3,870,815 to 3,871,723
cysD
SO3728: cobA - uroporphyrin-III C-methyltransferase (NCBI ptt file), at 3,871,804 to 3,872,634
cobA
Position (kb)
3870
3871
3872 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3870.288 kb on + strand, within cysN at 3870.288 kb on + strand, within cysN at 3870.288 kb on + strand, within cysN at 3870.288 kb on + strand, within cysN at 3870.296 kb on - strand, within cysN at 3870.296 kb on - strand, within cysN at 3870.438 kb on + strand, within cysN at 3870.439 kb on - strand, within cysN at 3870.518 kb on - strand at 3870.565 kb on + strand at 3870.574 kb on - strand at 3870.574 kb on - strand at 3870.974 kb on + strand, within cysD at 3870.974 kb on + strand, within cysD at 3870.982 kb on - strand, within cysD at 3871.082 kb on - strand, within cysD at 3871.228 kb on - strand, within cysD at 3871.306 kb on - strand, within cysD at 3871.364 kb on + strand, within cysD at 3871.404 kb on - strand, within cysD at 3871.404 kb on - strand, within cysD at 3871.404 kb on - strand, within cysD at 3871.428 kb on - strand, within cysD at 3871.505 kb on + strand, within cysD at 3871.719 kb on + strand at 3871.719 kb on + strand at 3871.719 kb on + strand at 3871.719 kb on + strand at 3871.727 kb on - strand at 3871.727 kb on - strand at 3871.740 kb on - strand at 3871.757 kb on - strand at 3871.767 kb on - strand at 3871.791 kb on - strand at 3871.829 kb on + strand at 3871.847 kb on - strand at 3871.870 kb on - strand at 3871.897 kb on + strand, within cobA at 3871.949 kb on + strand, within cobA at 3872.064 kb on - strand, within cobA at 3872.126 kb on + strand, within cobA at 3872.134 kb on - strand, within cobA at 3872.176 kb on + strand, within cobA at 3872.305 kb on - strand, within cobA at 3872.511 kb on - strand, within cobA at 3872.586 kb on + strand at 3872.590 kb on + strand at 3872.659 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 3,870,288 + cysN SO3726 0.76 -3.9 3,870,288 + cysN SO3726 0.76 -3.5 3,870,288 + cysN SO3726 0.76 -4.1 3,870,288 + cysN SO3726 0.76 -2.9 3,870,296 - cysN SO3726 0.77 -4.1 3,870,296 - cysN SO3726 0.77 -4.6 3,870,438 + cysN SO3726 0.87 -3.2 3,870,439 - cysN SO3726 0.87 -2.7 3,870,518 - -0.2 3,870,565 + -1.8 3,870,574 - -3.9 3,870,574 - -0.9 3,870,974 + cysD SO3727 0.17 -3.7 3,870,974 + cysD SO3727 0.17 -1.8 3,870,982 - cysD SO3727 0.18 -3.7 3,871,082 - cysD SO3727 0.29 -4.3 3,871,228 - cysD SO3727 0.45 -3.3 3,871,306 - cysD SO3727 0.54 -3.1 3,871,364 + cysD SO3727 0.60 +0.4 3,871,404 - cysD SO3727 0.65 -6.4 3,871,404 - cysD SO3727 0.65 -4.4 3,871,404 - cysD SO3727 0.65 -4.6 3,871,428 - cysD SO3727 0.67 -4.5 3,871,505 + cysD SO3727 0.76 -4.8 3,871,719 + -3.2 3,871,719 + -4.2 3,871,719 + -2.6 3,871,719 + -4.2 3,871,727 - -4.0 3,871,727 - -0.6 3,871,740 - -1.8 3,871,757 - -5.8 3,871,767 - -2.7 3,871,791 - -4.0 3,871,829 + -2.3 3,871,847 - -2.2 3,871,870 - -2.7 3,871,897 + cobA SO3728 0.11 -4.0 3,871,949 + cobA SO3728 0.17 -3.2 3,872,064 - cobA SO3728 0.31 -2.7 3,872,126 + cobA SO3728 0.39 -3.4 3,872,134 - cobA SO3728 0.40 -4.4 3,872,176 + cobA SO3728 0.45 -3.4 3,872,305 - cobA SO3728 0.60 -2.2 3,872,511 - cobA SO3728 0.85 -2.3 3,872,586 + -2.2 3,872,590 + -3.0 3,872,659 - -1.5
Or see this region's nucleotide sequence