Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrB and thrA overlap by 4 nucleotides thrA and slyD are separated by 641 nucleotides
SO3414: thrB - homoserine kinase (NCBI ptt file), at 3,554,272 to 3,555,210
thrB
SO3415: thrA - aspartokinase I/homoserine dehydrogenase, threonine-sensitive (NCBI ptt file), at 3,555,207 to 3,557,675
thrA
SO3417: slyD - peptidyl-prolyl cis-trans isomerase SlyD (NCBI ptt file), at 3,558,317 to 3,558,985
slyD
Position (kb)
3555
3556
3557
3558 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3554.221 kb on - strand at 3554.224 kb on + strand at 3554.232 kb on - strand at 3554.244 kb on + strand at 3554.279 kb on + strand at 3554.279 kb on + strand at 3554.279 kb on + strand at 3554.481 kb on - strand, within thrB at 3554.498 kb on + strand, within thrB at 3554.505 kb on - strand, within thrB at 3554.556 kb on + strand, within thrB at 3554.612 kb on - strand, within thrB at 3554.612 kb on - strand, within thrB at 3554.656 kb on + strand, within thrB at 3554.689 kb on - strand, within thrB at 3554.689 kb on - strand, within thrB at 3554.694 kb on + strand, within thrB at 3554.694 kb on + strand, within thrB at 3554.784 kb on + strand, within thrB at 3554.795 kb on - strand, within thrB at 3554.892 kb on + strand, within thrB at 3554.911 kb on + strand, within thrB at 3554.918 kb on - strand, within thrB at 3554.955 kb on - strand, within thrB at 3555.075 kb on + strand, within thrB at 3555.092 kb on + strand, within thrB at 3555.206 kb on + strand at 3555.206 kb on + strand at 3555.218 kb on + strand at 3555.224 kb on - strand at 3555.280 kb on - strand at 3555.295 kb on - strand at 3555.305 kb on - strand at 3555.310 kb on - strand at 3555.398 kb on - strand at 3555.439 kb on - strand at 3555.448 kb on + strand at 3555.448 kb on + strand at 3555.456 kb on - strand, within thrA at 3555.456 kb on - strand, within thrA at 3555.638 kb on + strand, within thrA at 3555.726 kb on - strand, within thrA at 3555.780 kb on - strand, within thrA at 3555.878 kb on + strand, within thrA at 3556.011 kb on - strand, within thrA at 3556.027 kb on + strand, within thrA at 3556.058 kb on + strand, within thrA at 3556.230 kb on + strand, within thrA at 3556.230 kb on + strand, within thrA at 3556.234 kb on - strand, within thrA at 3556.271 kb on + strand, within thrA at 3556.299 kb on + strand, within thrA at 3556.330 kb on + strand, within thrA at 3556.330 kb on + strand, within thrA at 3556.330 kb on + strand, within thrA at 3556.330 kb on + strand, within thrA at 3556.330 kb on + strand, within thrA at 3556.338 kb on - strand, within thrA at 3556.338 kb on - strand, within thrA at 3556.338 kb on - strand, within thrA at 3556.364 kb on + strand, within thrA at 3556.364 kb on + strand, within thrA at 3556.418 kb on - strand, within thrA at 3556.442 kb on + strand, within thrA at 3556.512 kb on + strand, within thrA at 3556.859 kb on + strand, within thrA at 3556.875 kb on - strand, within thrA at 3556.875 kb on - strand, within thrA at 3557.046 kb on + strand, within thrA at 3557.064 kb on - strand, within thrA at 3557.064 kb on - strand, within thrA at 3557.070 kb on + strand, within thrA at 3557.070 kb on + strand, within thrA at 3557.176 kb on - strand, within thrA at 3557.233 kb on - strand, within thrA at 3557.256 kb on - strand, within thrA at 3557.424 kb on + strand, within thrA at 3557.437 kb on - strand at 3557.547 kb on - strand at 3557.573 kb on - strand at 3557.682 kb on + strand at 3557.690 kb on - strand at 3557.711 kb on - strand at 3557.721 kb on - strand at 3557.766 kb on - strand at 3557.779 kb on - strand at 3557.842 kb on - strand at 3557.851 kb on + strand at 3557.859 kb on - strand at 3557.859 kb on - strand at 3558.135 kb on - strand at 3558.227 kb on - strand at 3558.306 kb on - strand at 3558.549 kb on - strand, within slyD
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 3,554,221 - -0.7 3,554,224 + -5.1 3,554,232 - -2.4 3,554,244 + -3.2 3,554,279 + -4.8 3,554,279 + -4.4 3,554,279 + -4.1 3,554,481 - thrB SO3414 0.22 -2.9 3,554,498 + thrB SO3414 0.24 -4.3 3,554,505 - thrB SO3414 0.25 -3.8 3,554,556 + thrB SO3414 0.30 -2.8 3,554,612 - thrB SO3414 0.36 -3.0 3,554,612 - thrB SO3414 0.36 -5.1 3,554,656 + thrB SO3414 0.41 -3.9 3,554,689 - thrB SO3414 0.44 -4.1 3,554,689 - thrB SO3414 0.44 -2.6 3,554,694 + thrB SO3414 0.45 -3.9 3,554,694 + thrB SO3414 0.45 -4.1 3,554,784 + thrB SO3414 0.55 -0.7 3,554,795 - thrB SO3414 0.56 -4.1 3,554,892 + thrB SO3414 0.66 -5.2 3,554,911 + thrB SO3414 0.68 -4.3 3,554,918 - thrB SO3414 0.69 -3.4 3,554,955 - thrB SO3414 0.73 -5.2 3,555,075 + thrB SO3414 0.86 -3.6 3,555,092 + thrB SO3414 0.87 -5.0 3,555,206 + -4.4 3,555,206 + -4.4 3,555,218 + -3.6 3,555,224 - +0.2 3,555,280 - -1.2 3,555,295 - -0.4 3,555,305 - -0.8 3,555,310 - -1.1 3,555,398 - +0.2 3,555,439 - -2.6 3,555,448 + -3.4 3,555,448 + -3.8 3,555,456 - thrA SO3415 0.10 -1.1 3,555,456 - thrA SO3415 0.10 -0.7 3,555,638 + thrA SO3415 0.17 -4.1 3,555,726 - thrA SO3415 0.21 -1.2 3,555,780 - thrA SO3415 0.23 -1.3 3,555,878 + thrA SO3415 0.27 -3.4 3,556,011 - thrA SO3415 0.33 -0.2 3,556,027 + thrA SO3415 0.33 -3.2 3,556,058 + thrA SO3415 0.34 -4.7 3,556,230 + thrA SO3415 0.41 -3.5 3,556,230 + thrA SO3415 0.41 -4.3 3,556,234 - thrA SO3415 0.42 -2.1 3,556,271 + thrA SO3415 0.43 -3.0 3,556,299 + thrA SO3415 0.44 -5.8 3,556,330 + thrA SO3415 0.45 -3.4 3,556,330 + thrA SO3415 0.45 -0.5 3,556,330 + thrA SO3415 0.45 -3.6 3,556,330 + thrA SO3415 0.45 -4.6 3,556,330 + thrA SO3415 0.45 -4.2 3,556,338 - thrA SO3415 0.46 -1.4 3,556,338 - thrA SO3415 0.46 -1.4 3,556,338 - thrA SO3415 0.46 -1.3 3,556,364 + thrA SO3415 0.47 -4.3 3,556,364 + thrA SO3415 0.47 -3.6 3,556,418 - thrA SO3415 0.49 -1.7 3,556,442 + thrA SO3415 0.50 -2.3 3,556,512 + thrA SO3415 0.53 -4.1 3,556,859 + thrA SO3415 0.67 -2.6 3,556,875 - thrA SO3415 0.68 -3.0 3,556,875 - thrA SO3415 0.68 -4.6 3,557,046 + thrA SO3415 0.74 -2.4 3,557,064 - thrA SO3415 0.75 -3.1 3,557,064 - thrA SO3415 0.75 -3.3 3,557,070 + thrA SO3415 0.75 -4.1 3,557,070 + thrA SO3415 0.75 -3.2 3,557,176 - thrA SO3415 0.80 -2.7 3,557,233 - thrA SO3415 0.82 -2.8 3,557,256 - thrA SO3415 0.83 -4.2 3,557,424 + thrA SO3415 0.90 -4.2 3,557,437 - -3.7 3,557,547 - -1.5 3,557,573 - -2.6 3,557,682 + -3.8 3,557,690 - +0.1 3,557,711 - +0.5 3,557,721 - +0.6 3,557,766 - -5.2 3,557,779 - -4.7 3,557,842 - -0.9 3,557,851 + -2.8 3,557,859 - -1.5 3,557,859 - -2.4 3,558,135 - -0.2 3,558,227 - +0.2 3,558,306 - +1.4 3,558,549 - slyD SO3417 0.35 +0.7
Or see this region's nucleotide sequence