Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcm and surE overlap by 4 nucleotides surE and SO3436 overlap by 1 nucleotides SO3436 and ispF are separated by 28 nucleotides ispF and ispD are separated by 52 nucleotides
SO3434: pcm - protein-L-isoaspartate O-methyltransferase (NCBI ptt file), at 3,579,274 to 3,579,909
pcm
SO3435: surE - stationary-phase survival protein SurE (NCBI ptt file), at 3,579,906 to 3,580,652
surE
SO3436: SO3436 - conserved hypothetical protein (NCBI ptt file), at 3,580,652 to 3,581,764
SO3436
SO3437: ispF - 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (NCBI ptt file), at 3,581,793 to 3,582,272
ispF
SO3438: ispD - 4-diphosphocytidyl-2C-methyl-D-erythritol synthase (NCBI ptt file), at 3,582,325 to 3,583,074
ispD
Position (kb)
3580
3581
3582 Strain fitness (log2 ratio)
-2
-1
0
1 at 3579.655 kb on - strand, within pcm at 3579.721 kb on - strand, within pcm at 3579.736 kb on + strand, within pcm at 3579.744 kb on - strand, within pcm at 3579.750 kb on - strand, within pcm at 3580.043 kb on + strand, within surE at 3580.141 kb on + strand, within surE at 3580.218 kb on + strand, within surE at 3580.218 kb on + strand, within surE at 3580.262 kb on + strand, within surE at 3580.411 kb on + strand, within surE at 3580.422 kb on - strand, within surE at 3580.429 kb on - strand, within surE at 3580.553 kb on + strand, within surE at 3580.652 kb on + strand at 3580.695 kb on + strand at 3580.703 kb on - strand at 3580.733 kb on - strand at 3580.831 kb on - strand, within SO3436 at 3580.920 kb on - strand, within SO3436 at 3581.068 kb on - strand, within SO3436 at 3581.106 kb on + strand, within SO3436 at 3581.168 kb on + strand, within SO3436 at 3581.176 kb on - strand, within SO3436 at 3581.181 kb on - strand, within SO3436 at 3581.398 kb on - strand, within SO3436 at 3581.510 kb on + strand, within SO3436 at 3581.518 kb on - strand, within SO3436 at 3581.645 kb on + strand, within SO3436 at 3581.724 kb on + strand at 3581.745 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 3,579,655 - pcm SO3434 0.60 -0.5 3,579,721 - pcm SO3434 0.70 -0.5 3,579,736 + pcm SO3434 0.73 -0.8 3,579,744 - pcm SO3434 0.74 -2.3 3,579,750 - pcm SO3434 0.75 -2.0 3,580,043 + surE SO3435 0.18 -0.6 3,580,141 + surE SO3435 0.31 -0.5 3,580,218 + surE SO3435 0.42 -2.0 3,580,218 + surE SO3435 0.42 -2.3 3,580,262 + surE SO3435 0.48 -0.5 3,580,411 + surE SO3435 0.68 -0.2 3,580,422 - surE SO3435 0.69 -1.7 3,580,429 - surE SO3435 0.70 -2.3 3,580,553 + surE SO3435 0.87 -1.4 3,580,652 + -1.5 3,580,695 + -0.9 3,580,703 - -0.1 3,580,733 - -0.3 3,580,831 - SO3436 0.16 -0.4 3,580,920 - SO3436 0.24 -1.2 3,581,068 - SO3436 0.37 -0.1 3,581,106 + SO3436 0.41 -1.4 3,581,168 + SO3436 0.46 -1.7 3,581,176 - SO3436 0.47 +0.0 3,581,181 - SO3436 0.48 -0.9 3,581,398 - SO3436 0.67 -1.2 3,581,510 + SO3436 0.77 -1.0 3,581,518 - SO3436 0.78 -1.7 3,581,645 + SO3436 0.89 -1.3 3,581,724 + -0.8 3,581,745 + -1.3
Or see this region's nucleotide sequence