Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheV-3 and SO3253 are separated by 205 nucleotides SO3253 and flgM are separated by 75 nucleotides flgM and flgN are separated by 9 nucleotides flgN and SO3256 are separated by 117 nucleotides
SO3252: cheV-3 - chemotaxis protein CheV (NCBI ptt file), at 3,389,778 to 3,390,698
cheV-3
SO3253: SO3253 - flagellar basal-body P-ring formation protein FlgA, putative (NCBI ptt file), at 3,390,904 to 3,391,611
SO3253
SO3254: flgM - negative regulator of flagellin synthesis FlgM (NCBI ptt file), at 3,391,687 to 3,392,007
flgM
SO3255: flgN - flagellar biosynthetic protein FlgN (NCBI ptt file), at 3,392,017 to 3,392,442
flgN
SO3256: SO3256 - conserved hypothetical protein (NCBI ptt file), at 3,392,560 to 3,393,009
SO3256
Position (kb)
3390
3391
3392 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3389.940 kb on + strand, within cheV-3 at 3389.940 kb on + strand, within cheV-3 at 3389.958 kb on - strand, within cheV-3 at 3390.067 kb on - strand, within cheV-3 at 3390.120 kb on - strand, within cheV-3 at 3390.162 kb on + strand, within cheV-3 at 3390.188 kb on + strand, within cheV-3 at 3390.188 kb on + strand, within cheV-3 at 3390.188 kb on + strand, within cheV-3 at 3390.201 kb on - strand, within cheV-3 at 3390.310 kb on - strand, within cheV-3 at 3390.315 kb on - strand, within cheV-3 at 3390.315 kb on - strand, within cheV-3 at 3390.328 kb on + strand, within cheV-3 at 3390.335 kb on + strand, within cheV-3 at 3390.343 kb on - strand, within cheV-3 at 3390.351 kb on + strand, within cheV-3 at 3390.383 kb on + strand, within cheV-3 at 3390.398 kb on - strand, within cheV-3 at 3390.456 kb on + strand, within cheV-3 at 3390.456 kb on + strand, within cheV-3 at 3390.456 kb on + strand, within cheV-3 at 3390.464 kb on - strand, within cheV-3 at 3390.517 kb on + strand, within cheV-3 at 3390.525 kb on - strand, within cheV-3 at 3390.525 kb on - strand, within cheV-3 at 3390.529 kb on + strand, within cheV-3 at 3390.540 kb on + strand, within cheV-3 at 3390.549 kb on + strand, within cheV-3 at 3390.557 kb on - strand, within cheV-3 at 3390.635 kb on + strand at 3390.643 kb on - strand at 3390.645 kb on + strand at 3390.645 kb on + strand at 3390.653 kb on - strand at 3390.653 kb on - strand at 3390.695 kb on + strand at 3390.695 kb on + strand at 3390.703 kb on - strand at 3390.705 kb on + strand at 3390.708 kb on + strand at 3390.710 kb on + strand at 3390.718 kb on + strand at 3390.844 kb on - strand at 3390.846 kb on + strand at 3390.864 kb on + strand at 3390.872 kb on - strand at 3390.882 kb on - strand at 3390.974 kb on + strand at 3390.974 kb on + strand at 3390.974 kb on + strand at 3390.976 kb on + strand, within SO3253 at 3390.984 kb on - strand, within SO3253 at 3390.994 kb on - strand, within SO3253 at 3390.996 kb on - strand, within SO3253 at 3391.084 kb on - strand, within SO3253 at 3391.121 kb on + strand, within SO3253 at 3391.155 kb on + strand, within SO3253 at 3391.155 kb on + strand, within SO3253 at 3391.163 kb on + strand, within SO3253 at 3391.196 kb on - strand, within SO3253 at 3391.199 kb on + strand, within SO3253 at 3391.202 kb on - strand, within SO3253 at 3391.202 kb on - strand, within SO3253 at 3391.258 kb on + strand, within SO3253 at 3391.258 kb on + strand, within SO3253 at 3391.263 kb on + strand, within SO3253 at 3391.264 kb on - strand, within SO3253 at 3391.265 kb on + strand, within SO3253 at 3391.265 kb on + strand, within SO3253 at 3391.273 kb on - strand, within SO3253 at 3391.273 kb on - strand, within SO3253 at 3391.289 kb on + strand, within SO3253 at 3391.289 kb on + strand, within SO3253 at 3391.308 kb on - strand, within SO3253 at 3391.337 kb on + strand, within SO3253 at 3391.362 kb on + strand, within SO3253 at 3391.362 kb on + strand, within SO3253 at 3391.370 kb on - strand, within SO3253 at 3391.370 kb on - strand, within SO3253 at 3391.370 kb on - strand, within SO3253 at 3391.370 kb on - strand, within SO3253 at 3391.383 kb on + strand, within SO3253 at 3391.391 kb on - strand, within SO3253 at 3391.422 kb on + strand, within SO3253 at 3391.496 kb on + strand, within SO3253 at 3391.510 kb on - strand, within SO3253 at 3391.616 kb on + strand at 3391.667 kb on + strand at 3391.731 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.845 kb on + strand, within flgM at 3391.845 kb on + strand, within flgM at 3391.987 kb on + strand at 3392.042 kb on + strand at 3392.253 kb on + strand, within flgN at 3392.266 kb on - strand, within flgN at 3392.266 kb on - strand, within flgN at 3392.336 kb on + strand, within flgN at 3392.341 kb on + strand, within flgN at 3392.349 kb on - strand, within flgN at 3392.394 kb on + strand, within flgN at 3392.529 kb on - strand at 3392.530 kb on + strand at 3392.540 kb on + strand at 3392.548 kb on - strand at 3392.548 kb on - strand at 3392.548 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 3,389,940 + cheV-3 SO3252 0.18 +0.8 3,389,940 + cheV-3 SO3252 0.18 -1.1 3,389,958 - cheV-3 SO3252 0.20 -0.5 3,390,067 - cheV-3 SO3252 0.31 +0.0 3,390,120 - cheV-3 SO3252 0.37 +1.0 3,390,162 + cheV-3 SO3252 0.42 -0.2 3,390,188 + cheV-3 SO3252 0.45 +0.4 3,390,188 + cheV-3 SO3252 0.45 -0.3 3,390,188 + cheV-3 SO3252 0.45 -0.2 3,390,201 - cheV-3 SO3252 0.46 -0.1 3,390,310 - cheV-3 SO3252 0.58 +0.4 3,390,315 - cheV-3 SO3252 0.58 +0.8 3,390,315 - cheV-3 SO3252 0.58 +1.0 3,390,328 + cheV-3 SO3252 0.60 -0.8 3,390,335 + cheV-3 SO3252 0.60 +0.6 3,390,343 - cheV-3 SO3252 0.61 +0.6 3,390,351 + cheV-3 SO3252 0.62 +0.2 3,390,383 + cheV-3 SO3252 0.66 +0.3 3,390,398 - cheV-3 SO3252 0.67 -1.4 3,390,456 + cheV-3 SO3252 0.74 -0.9 3,390,456 + cheV-3 SO3252 0.74 -0.9 3,390,456 + cheV-3 SO3252 0.74 -0.0 3,390,464 - cheV-3 SO3252 0.74 +1.5 3,390,517 + cheV-3 SO3252 0.80 -0.5 3,390,525 - cheV-3 SO3252 0.81 -0.5 3,390,525 - cheV-3 SO3252 0.81 -0.1 3,390,529 + cheV-3 SO3252 0.82 -0.6 3,390,540 + cheV-3 SO3252 0.83 +0.1 3,390,549 + cheV-3 SO3252 0.84 +1.0 3,390,557 - cheV-3 SO3252 0.85 +0.4 3,390,635 + +0.1 3,390,643 - -1.4 3,390,645 + +0.9 3,390,645 + +0.2 3,390,653 - +0.5 3,390,653 - -0.6 3,390,695 + -0.5 3,390,695 + -0.0 3,390,703 - +0.2 3,390,705 + +0.3 3,390,708 + -0.1 3,390,710 + -0.3 3,390,718 + -0.0 3,390,844 - -2.0 3,390,846 + +0.1 3,390,864 + +0.6 3,390,872 - -0.9 3,390,882 - -1.6 3,390,974 + -0.2 3,390,974 + -1.7 3,390,974 + -1.0 3,390,976 + SO3253 0.10 -1.3 3,390,984 - SO3253 0.11 -1.3 3,390,994 - SO3253 0.13 -0.6 3,390,996 - SO3253 0.13 -1.6 3,391,084 - SO3253 0.25 -0.6 3,391,121 + SO3253 0.31 -1.8 3,391,155 + SO3253 0.35 -0.5 3,391,155 + SO3253 0.35 -0.4 3,391,163 + SO3253 0.37 -1.8 3,391,196 - SO3253 0.41 -0.9 3,391,199 + SO3253 0.42 -0.1 3,391,202 - SO3253 0.42 -1.3 3,391,202 - SO3253 0.42 -0.8 3,391,258 + SO3253 0.50 -0.4 3,391,258 + SO3253 0.50 -0.1 3,391,263 + SO3253 0.51 -1.1 3,391,264 - SO3253 0.51 +0.8 3,391,265 + SO3253 0.51 -2.2 3,391,265 + SO3253 0.51 -1.3 3,391,273 - SO3253 0.52 -3.6 3,391,273 - SO3253 0.52 -2.8 3,391,289 + SO3253 0.54 -0.2 3,391,289 + SO3253 0.54 -0.4 3,391,308 - SO3253 0.57 -2.2 3,391,337 + SO3253 0.61 -0.8 3,391,362 + SO3253 0.65 +0.6 3,391,362 + SO3253 0.65 -0.6 3,391,370 - SO3253 0.66 -1.6 3,391,370 - SO3253 0.66 -0.6 3,391,370 - SO3253 0.66 -1.7 3,391,370 - SO3253 0.66 -1.4 3,391,383 + SO3253 0.68 -1.0 3,391,391 - SO3253 0.69 -1.0 3,391,422 + SO3253 0.73 -0.5 3,391,496 + SO3253 0.84 -2.6 3,391,510 - SO3253 0.86 -1.4 3,391,616 + +0.6 3,391,667 + -0.7 3,391,731 + flgM SO3254 0.14 -2.5 3,391,745 + flgM SO3254 0.18 -0.4 3,391,745 + flgM SO3254 0.18 -0.1 3,391,745 + flgM SO3254 0.18 -0.3 3,391,745 + flgM SO3254 0.18 +0.6 3,391,745 + flgM SO3254 0.18 -1.6 3,391,745 + flgM SO3254 0.18 +0.1 3,391,745 + flgM SO3254 0.18 -0.5 3,391,745 + flgM SO3254 0.18 -1.2 3,391,745 + flgM SO3254 0.18 -0.4 3,391,745 + flgM SO3254 0.18 +0.0 3,391,845 + flgM SO3254 0.49 +0.2 3,391,845 + flgM SO3254 0.49 +0.6 3,391,987 + -0.6 3,392,042 + -1.5 3,392,253 + flgN SO3255 0.55 -0.8 3,392,266 - flgN SO3255 0.58 -1.9 3,392,266 - flgN SO3255 0.58 -0.8 3,392,336 + flgN SO3255 0.75 -0.2 3,392,341 + flgN SO3255 0.76 -0.5 3,392,349 - flgN SO3255 0.78 -0.8 3,392,394 + flgN SO3255 0.88 +0.7 3,392,529 - +0.9 3,392,530 + +0.8 3,392,540 + +0.5 3,392,548 - +0.4 3,392,548 - -0.0 3,392,548 - +0.5
Or see this region's nucleotide sequence