Strain Fitness in Shewanella oneidensis MR-1 around SO3213

Experiment: methylglyoxal 0.004 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflhF and flhA are separated by 11 nucleotidesflhA and flhB are separated by 377 nucleotides SO3212: flhF - flagellar biosynthetic protein FlhF (NCBI ptt file), at 3,351,747 to 3,353,123 flhF SO3213: flhA - flagellar biosynthesis protein FlhA (NCBI ptt file), at 3,353,135 to 3,355,234 flhA SO3215: flhB - flagellar biosynthetic protein FlhB (NCBI ptt file), at 3,355,612 to 3,356,742 flhB Position (kb) 3353 3354 3355 3356Strain fitness (log2 ratio) -2 -1 0 1at 3352.260 kb on + strand, within flhFat 3352.310 kb on - strand, within flhFat 3352.383 kb on + strand, within flhFat 3352.621 kb on - strand, within flhFat 3352.690 kb on + strand, within flhFat 3352.743 kb on - strand, within flhFat 3352.896 kb on + strand, within flhFat 3353.048 kb on + strandat 3353.056 kb on - strandat 3353.143 kb on - strandat 3353.234 kb on + strandat 3353.262 kb on - strandat 3353.506 kb on + strand, within flhAat 3353.689 kb on - strand, within flhAat 3353.788 kb on + strand, within flhAat 3353.795 kb on + strand, within flhAat 3353.906 kb on - strand, within flhAat 3353.986 kb on + strand, within flhAat 3354.057 kb on + strand, within flhAat 3354.057 kb on + strand, within flhAat 3354.065 kb on - strand, within flhAat 3354.301 kb on - strand, within flhAat 3354.317 kb on + strand, within flhAat 3354.406 kb on - strand, within flhAat 3354.476 kb on - strand, within flhAat 3354.503 kb on - strand, within flhAat 3354.544 kb on - strand, within flhAat 3354.545 kb on + strand, within flhAat 3354.620 kb on - strand, within flhAat 3354.684 kb on - strand, within flhAat 3354.695 kb on + strand, within flhAat 3354.884 kb on - strand, within flhAat 3354.907 kb on - strand, within flhAat 3354.949 kb on + strand, within flhAat 3354.969 kb on + strand, within flhAat 3355.012 kb on - strand, within flhAat 3355.036 kb on - strandat 3355.076 kb on - strandat 3355.076 kb on - strandat 3355.209 kb on - strandat 3355.211 kb on + strandat 3355.218 kb on - strandat 3355.333 kb on - strandat 3355.336 kb on - strandat 3355.610 kb on + strandat 3355.610 kb on + strandat 3355.641 kb on + strandat 3355.661 kb on + strandat 3355.740 kb on - strand, within flhBat 3355.761 kb on + strand, within flhBat 3355.785 kb on + strand, within flhBat 3355.897 kb on - strand, within flhBat 3356.030 kb on + strand, within flhBat 3356.030 kb on + strand, within flhBat 3356.038 kb on - strand, within flhBat 3356.088 kb on + strand, within flhBat 3356.142 kb on - strand, within flhBat 3356.142 kb on - strand, within flhB

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol%
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3,352,260 + flhF SO3212 0.37 +1.2
3,352,310 - flhF SO3212 0.41 +0.2
3,352,383 + flhF SO3212 0.46 -1.1
3,352,621 - flhF SO3212 0.63 +1.5
3,352,690 + flhF SO3212 0.68 -0.9
3,352,743 - flhF SO3212 0.72 -1.0
3,352,896 + flhF SO3212 0.83 -0.4
3,353,048 + -1.2
3,353,056 - -0.3
3,353,143 - -0.5
3,353,234 + -0.9
3,353,262 - -0.9
3,353,506 + flhA SO3213 0.18 -0.5
3,353,689 - flhA SO3213 0.26 -0.9
3,353,788 + flhA SO3213 0.31 -1.4
3,353,795 + flhA SO3213 0.31 -2.0
3,353,906 - flhA SO3213 0.37 -0.5
3,353,986 + flhA SO3213 0.41 -1.4
3,354,057 + flhA SO3213 0.44 -1.2
3,354,057 + flhA SO3213 0.44 -1.7
3,354,065 - flhA SO3213 0.44 -0.1
3,354,301 - flhA SO3213 0.56 -0.6
3,354,317 + flhA SO3213 0.56 -0.8
3,354,406 - flhA SO3213 0.61 -1.5
3,354,476 - flhA SO3213 0.64 -0.8
3,354,503 - flhA SO3213 0.65 -0.8
3,354,544 - flhA SO3213 0.67 -1.5
3,354,545 + flhA SO3213 0.67 -1.0
3,354,620 - flhA SO3213 0.71 -2.4
3,354,684 - flhA SO3213 0.74 +0.6
3,354,695 + flhA SO3213 0.74 -0.4
3,354,884 - flhA SO3213 0.83 -0.8
3,354,907 - flhA SO3213 0.84 -1.2
3,354,949 + flhA SO3213 0.86 -1.0
3,354,969 + flhA SO3213 0.87 -0.9
3,355,012 - flhA SO3213 0.89 -1.6
3,355,036 - -1.3
3,355,076 - -0.4
3,355,076 - -0.8
3,355,209 - +0.8
3,355,211 + -1.6
3,355,218 - -0.6
3,355,333 - +0.2
3,355,336 - -0.6
3,355,610 + +0.8
3,355,610 + -0.0
3,355,641 + -0.9
3,355,661 + -0.3
3,355,740 - flhB SO3215 0.11 -0.8
3,355,761 + flhB SO3215 0.13 -2.1
3,355,785 + flhB SO3215 0.15 -1.1
3,355,897 - flhB SO3215 0.25 -0.8
3,356,030 + flhB SO3215 0.37 -0.6
3,356,030 + flhB SO3215 0.37 -0.3
3,356,038 - flhB SO3215 0.38 -1.2
3,356,088 + flhB SO3215 0.42 -1.3
3,356,142 - flhB SO3215 0.47 -0.8
3,356,142 - flhB SO3215 0.47 -2.2

Or see this region's nucleotide sequence