Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2512 and SO2513 overlap by 8 nucleotides SO2513 and nth are separated by 73 nucleotides nth and SO2515 are separated by 29 nucleotides SO2515 and SO2516 overlap by 2 nucleotides SO2516 and SO2517 are separated by 131 nucleotides
SO2512: SO2512 - conserved hypothetical protein (NCBI ptt file), at 2,640,329 to 2,640,967
SO2512
SO2513: SO2513 - NqrDE/RnfAE family protein (NCBI ptt file), at 2,640,960 to 2,641,658
SO2513
SO2514: nth - endonuclease III (NCBI ptt file), at 2,641,732 to 2,642,427
nth
SO2515: SO2515 - hypothetical protein (NCBI ptt file), at 2,642,457 to 2,642,729
SO2515
SO2516: SO2516 - hypothetical protein (NCBI ptt file), at 2,642,728 to 2,642,916
SO2516
SO2517: SO2517 - hypothetical protein (NCBI ptt file), at 2,643,048 to 2,643,974
SO2517
Position (kb)
2641
2642
2643 Strain fitness (log2 ratio)
-1
0
1 at 2641.907 kb on + strand, within nth at 2642.007 kb on - strand, within nth at 2642.146 kb on + strand, within nth at 2642.259 kb on - strand, within nth at 2642.272 kb on - strand, within nth at 2642.366 kb on + strand at 2642.366 kb on + strand at 2642.439 kb on - strand at 2642.791 kb on - strand, within SO2516 at 2642.976 kb on - strand at 2643.020 kb on - strand at 2643.053 kb on - strand at 2643.063 kb on - strand at 2643.070 kb on - strand at 2643.155 kb on + strand, within SO2517 at 2643.338 kb on - strand at 2643.359 kb on + strand, within SO2517 at 2643.366 kb on + strand, within SO2517 at 2643.367 kb on - strand, within SO2517
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 2,641,907 + nth SO2514 0.25 +1.1 2,642,007 - nth SO2514 0.40 -0.4 2,642,146 + nth SO2514 0.59 -0.2 2,642,259 - nth SO2514 0.76 +0.5 2,642,272 - nth SO2514 0.78 -0.8 2,642,366 + +0.5 2,642,366 + -0.4 2,642,439 - -0.2 2,642,791 - SO2516 0.33 +0.8 2,642,976 - +0.3 2,643,020 - -0.7 2,643,053 - +0.5 2,643,063 - -0.6 2,643,070 - -0.4 2,643,155 + SO2517 0.12 -0.1 2,643,338 - -0.5 2,643,359 + SO2517 0.34 +0.0 2,643,366 + SO2517 0.34 +0.4 2,643,367 - SO2517 0.34 -0.0
Or see this region's nucleotide sequence