Strain Fitness in Shewanella oneidensis MR-1 around SO2013

Experiment: methylglyoxal 0.004 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntapt and dnaX are separated by 131 nucleotidesdnaX and SO2014 are separated by 229 nucleotidesSO2014 and recR are separated by 12 nucleotides SO2012: apt - adenine phosphoribosyltransferase (NCBI ptt file), at 2,109,294 to 2,109,839 apt SO2013: dnaX - DNA polymerase III, gamma and tau subunits (NCBI ptt file), at 2,109,971 to 2,112,979 dnaX SO2014: SO2014 - conserved hypothetical protein TIGR00103 (NCBI ptt file), at 2,113,209 to 2,113,538 SO2014 SO2015: recR - recombination protein RecR (NCBI ptt file), at 2,113,551 to 2,114,150 recR Position (kb) 2109 2110 2111 2112 2113Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2108.982 kb on - strandat 2109.148 kb on + strandat 2109.160 kb on + strandat 2109.185 kb on + strandat 2109.205 kb on + strandat 2109.314 kb on - strandat 2109.529 kb on + strand, within aptat 2109.608 kb on - strand, within aptat 2109.857 kb on + strandat 2112.883 kb on + strandat 2112.903 kb on + strandat 2112.918 kb on + strandat 2112.972 kb on - strandat 2113.040 kb on + strandat 2113.040 kb on + strandat 2113.040 kb on + strandat 2113.040 kb on + strandat 2113.048 kb on - strandat 2113.048 kb on - strandat 2113.048 kb on - strandat 2113.048 kb on - strandat 2113.048 kb on - strandat 2113.175 kb on - strandat 2113.246 kb on - strand, within SO2014at 2113.362 kb on - strand, within SO2014at 2113.576 kb on + strandat 2113.691 kb on + strand, within recRat 2113.691 kb on + strand, within recRat 2113.864 kb on + strand, within recRat 2113.920 kb on + strand, within recRat 2113.922 kb on - strand, within recR

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol%
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2,108,982 - -0.5
2,109,148 + +0.6
2,109,160 + +0.1
2,109,185 + +0.6
2,109,205 + -1.3
2,109,314 - +0.8
2,109,529 + apt SO2012 0.43 +0.8
2,109,608 - apt SO2012 0.58 +0.3
2,109,857 + -0.2
2,112,883 + +0.7
2,112,903 + -0.1
2,112,918 + +1.4
2,112,972 - -0.3
2,113,040 + -0.0
2,113,040 + +2.2
2,113,040 + -0.6
2,113,040 + -0.1
2,113,048 - +0.7
2,113,048 - -0.2
2,113,048 - +0.5
2,113,048 - -0.5
2,113,048 - +1.6
2,113,175 - -1.6
2,113,246 - SO2014 0.11 -1.7
2,113,362 - SO2014 0.46 -1.0
2,113,576 + -2.4
2,113,691 + recR SO2015 0.23 -3.2
2,113,691 + recR SO2015 0.23 -2.4
2,113,864 + recR SO2015 0.52 -4.0
2,113,920 + recR SO2015 0.61 -1.7
2,113,922 - recR SO2015 0.62 -2.9

Or see this region's nucleotide sequence