Strain Fitness in Shewanella oneidensis MR-1 around SO1820

Experiment: methylglyoxal 0.004 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdinG and polB are separated by 185 nucleotidespolB and SO1821 are separated by 220 nucleotides SO1819: dinG - ATP-dependent helicase DinG (NCBI ptt file), at 1,907,796 to 1,909,868 dinG SO1820: polB - DNA polymerase II (NCBI ptt file), at 1,910,054 to 1,912,480 polB SO1821: SO1821 - outer membrane porin, putative (NCBI ptt file), at 1,912,701 to 1,913,897 SO1821 Position (kb) 1910 1911 1912 1913Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1909.116 kb on + strand, within dinGat 1909.116 kb on + strand, within dinGat 1909.147 kb on + strand, within dinGat 1909.223 kb on - strand, within dinGat 1909.286 kb on + strand, within dinGat 1909.286 kb on + strand, within dinGat 1909.376 kb on - strand, within dinGat 1909.479 kb on - strand, within dinGat 1909.527 kb on - strand, within dinGat 1909.816 kb on + strandat 1909.904 kb on - strandat 1909.980 kb on - strandat 1909.991 kb on + strandat 1910.010 kb on - strandat 1910.033 kb on + strandat 1910.034 kb on - strandat 1910.042 kb on + strandat 1910.042 kb on + strandat 1910.050 kb on - strandat 1910.137 kb on - strandat 1910.156 kb on - strandat 1910.182 kb on + strandat 1910.267 kb on + strandat 1910.292 kb on - strandat 1910.312 kb on - strand, within polBat 1910.494 kb on - strand, within polBat 1910.621 kb on - strand, within polBat 1910.621 kb on - strand, within polBat 1910.652 kb on - strand, within polBat 1910.704 kb on - strand, within polBat 1910.718 kb on + strand, within polBat 1910.798 kb on - strand, within polBat 1911.152 kb on - strand, within polBat 1911.175 kb on - strand, within polBat 1911.196 kb on - strand, within polBat 1911.198 kb on + strand, within polBat 1911.206 kb on - strand, within polBat 1911.257 kb on + strand, within polBat 1911.385 kb on + strand, within polBat 1911.441 kb on - strand, within polBat 1911.548 kb on + strand, within polBat 1911.549 kb on + strand, within polBat 1911.557 kb on - strand, within polBat 1911.642 kb on + strand, within polBat 1911.684 kb on + strand, within polBat 1911.684 kb on + strand, within polBat 1911.825 kb on + strand, within polBat 1911.948 kb on + strand, within polBat 1911.948 kb on + strand, within polBat 1911.948 kb on + strand, within polBat 1911.956 kb on - strand, within polBat 1911.957 kb on + strand, within polBat 1911.957 kb on + strand, within polBat 1911.965 kb on - strand, within polBat 1911.965 kb on - strand, within polBat 1912.234 kb on - strand, within polBat 1912.330 kb on + strandat 1912.330 kb on + strandat 1912.335 kb on + strandat 1912.335 kb on + strandat 1912.894 kb on + strand, within SO1821at 1912.896 kb on - strand, within SO1821at 1912.973 kb on - strand, within SO1821at 1913.202 kb on + strand, within SO1821at 1913.294 kb on - strand, within SO1821at 1913.332 kb on + strand, within SO1821

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol%
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1,909,116 + dinG SO1819 0.64 -3.4
1,909,116 + dinG SO1819 0.64 -1.2
1,909,147 + dinG SO1819 0.65 -0.7
1,909,223 - dinG SO1819 0.69 -1.8
1,909,286 + dinG SO1819 0.72 -2.6
1,909,286 + dinG SO1819 0.72 -1.9
1,909,376 - dinG SO1819 0.76 -2.9
1,909,479 - dinG SO1819 0.81 -3.9
1,909,527 - dinG SO1819 0.84 -2.8
1,909,816 + -2.5
1,909,904 - -0.9
1,909,980 - +0.9
1,909,991 + +1.3
1,910,010 - +0.2
1,910,033 + +0.2
1,910,034 - -0.4
1,910,042 + +0.8
1,910,042 + +2.0
1,910,050 - -1.1
1,910,137 - +0.1
1,910,156 - -0.9
1,910,182 + -0.9
1,910,267 + +0.2
1,910,292 - +1.3
1,910,312 - polB SO1820 0.11 +0.5
1,910,494 - polB SO1820 0.18 -0.2
1,910,621 - polB SO1820 0.23 +0.2
1,910,621 - polB SO1820 0.23 -0.3
1,910,652 - polB SO1820 0.25 -0.1
1,910,704 - polB SO1820 0.27 +0.1
1,910,718 + polB SO1820 0.27 -0.2
1,910,798 - polB SO1820 0.31 -0.1
1,911,152 - polB SO1820 0.45 +0.7
1,911,175 - polB SO1820 0.46 -0.6
1,911,196 - polB SO1820 0.47 -0.1
1,911,198 + polB SO1820 0.47 +0.1
1,911,206 - polB SO1820 0.47 -1.7
1,911,257 + polB SO1820 0.50 -0.3
1,911,385 + polB SO1820 0.55 -0.5
1,911,441 - polB SO1820 0.57 +0.0
1,911,548 + polB SO1820 0.62 -0.9
1,911,549 + polB SO1820 0.62 +0.1
1,911,557 - polB SO1820 0.62 +0.0
1,911,642 + polB SO1820 0.65 +0.2
1,911,684 + polB SO1820 0.67 +0.5
1,911,684 + polB SO1820 0.67 +0.3
1,911,825 + polB SO1820 0.73 -0.0
1,911,948 + polB SO1820 0.78 -0.3
1,911,948 + polB SO1820 0.78 -1.0
1,911,948 + polB SO1820 0.78 -1.7
1,911,956 - polB SO1820 0.78 -1.3
1,911,957 + polB SO1820 0.78 -0.5
1,911,957 + polB SO1820 0.78 +0.4
1,911,965 - polB SO1820 0.79 +1.7
1,911,965 - polB SO1820 0.79 -1.0
1,912,234 - polB SO1820 0.90 -0.2
1,912,330 + -0.0
1,912,330 + +0.6
1,912,335 + -0.3
1,912,335 + +1.1
1,912,894 + SO1821 0.16 -0.7
1,912,896 - SO1821 0.16 -1.1
1,912,973 - SO1821 0.23 -0.7
1,913,202 + SO1821 0.42 -0.7
1,913,294 - SO1821 0.50 -1.1
1,913,332 + SO1821 0.53 -0.2

Or see this region's nucleotide sequence