Strain Fitness in Shewanella oneidensis MR-1 around SO1286

Experiment: methylglyoxal 0.004 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpoD and dnaG are separated by 135 nucleotidesdnaG and SO1287 are separated by 83 nucleotidesSO1287 and rpsU are separated by 6 nucleotidesrpsU and gcp are separated by 214 nucleotides SO1284: rpoD - RNA polymerase sigma-70 factor (NCBI ptt file), at 1,335,571 to 1,337,421 rpoD SO1286: dnaG - DNA primase (NCBI ptt file), at 1,337,557 to 1,339,281 dnaG SO1287: SO1287 - conserved hypothetical protein (NCBI ptt file), at 1,339,365 to 1,339,808 SO1287 SO1288: rpsU - ribosomal protein S21 (NCBI ptt file), at 1,339,815 to 1,340,030 rpsU SO1289: gcp - O-sialoglycoprotein endopeptidase (NCBI ptt file), at 1,340,245 to 1,341,261 gcp Position (kb) 1337 1338 1339 1340Strain fitness (log2 ratio) -1 0 1at 1339.424 kb on - strand, within SO1287at 1339.424 kb on - strand, within SO1287at 1340.127 kb on + strandat 1340.158 kb on - strandat 1340.159 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol%
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1,339,424 - SO1287 0.13 -0.8
1,339,424 - SO1287 0.13 +0.5
1,340,127 + +0.3
1,340,158 - -0.8
1,340,159 - +0.4

Or see this region's nucleotide sequence