Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glyS and glyQ are separated by 9 nucleotides glyQ and tag are separated by 117 nucleotides tag and SO0017 are separated by 86 nucleotides
SO0014: glyS - glycyl-tRNA synthetase, beta subunit (NCBI ptt file), at 15,180 to 17,246
glyS
SO0015: glyQ - glycyl-tRNA synthetase, alpha subunit (NCBI ptt file), at 17,256 to 18,161
glyQ
SO0016: tag - DNA-3-methyladenine glycosidase I (NCBI ptt file), at 18,279 to 18,854
tag
SO0017: SO0017 - hypothetical amidohydrolase (NCBI ptt file), at 18,941 to 22,090
SO0017
Position (kb) 17
18
19 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 18.212 kb on - strand at 18.270 kb on - strand at 18.386 kb on + strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.397 kb on - strand, within tag at 18.399 kb on - strand, within tag at 18.411 kb on + strand, within tag at 18.415 kb on - strand, within tag at 18.419 kb on - strand, within tag at 18.488 kb on - strand, within tag at 18.508 kb on + strand, within tag at 18.511 kb on - strand, within tag at 18.518 kb on + strand, within tag at 18.560 kb on + strand, within tag at 18.587 kb on + strand, within tag at 18.602 kb on + strand, within tag at 18.686 kb on + strand, within tag at 18.718 kb on + strand, within tag at 18.718 kb on + strand, within tag at 18.718 kb on + strand, within tag at 18.775 kb on + strand, within tag at 18.775 kb on + strand, within tag at 18.893 kb on + strand at 18.901 kb on - strand at 18.901 kb on - strand at 18.939 kb on + strand at 18.956 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.971 kb on - strand at 19.016 kb on - strand at 19.016 kb on - strand at 19.034 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 18,212 - +0.3 18,270 - -0.1 18,386 + tag SO0016 0.19 -0.0 18,394 - tag SO0016 0.20 -0.3 18,394 - tag SO0016 0.20 +2.1 18,394 - tag SO0016 0.20 -0.6 18,394 - tag SO0016 0.20 +0.4 18,394 - tag SO0016 0.20 +0.5 18,397 - tag SO0016 0.20 -0.2 18,399 - tag SO0016 0.21 +0.0 18,411 + tag SO0016 0.23 -1.2 18,415 - tag SO0016 0.24 -0.7 18,419 - tag SO0016 0.24 -0.3 18,488 - tag SO0016 0.36 +0.4 18,508 + tag SO0016 0.40 -0.4 18,511 - tag SO0016 0.40 -0.2 18,518 + tag SO0016 0.41 +0.3 18,560 + tag SO0016 0.49 +1.1 18,587 + tag SO0016 0.53 -0.5 18,602 + tag SO0016 0.56 +0.0 18,686 + tag SO0016 0.71 -0.5 18,718 + tag SO0016 0.76 +0.2 18,718 + tag SO0016 0.76 +2.1 18,718 + tag SO0016 0.76 +0.1 18,775 + tag SO0016 0.86 -0.0 18,775 + tag SO0016 0.86 -0.5 18,893 + -0.3 18,901 - -2.8 18,901 - +0.7 18,939 + +0.1 18,956 + -0.0 18,963 + +0.6 18,963 + -0.3 18,963 + +1.4 18,963 + -0.8 18,963 + -0.7 18,971 - -0.4 19,016 - +0.5 19,016 - -0.3 19,034 + +0.1
Or see this region's nucleotide sequence