Strain Fitness in Shewanella oneidensis MR-1 around SO0008

Experiment: methylglyoxal 0.004 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO0005 and rnpA overlap by 34 nucleotidesrnpA and rpmH are separated by 16 nucleotidesrpmH and dnaA are separated by 454 nucleotidesdnaA and dnaN are separated by 20 nucleotides SO0005: SO0005 - conserved hypothetical protein TIGR00278 (NCBI ptt file), at 5,687 to 5,941 SO0005 SO0006: rnpA - ribonuclease P protein component (NCBI ptt file), at 5,908 to 6,264 rnpA SO0007: rpmH - ribosomal protein L34 (NCBI ptt file), at 6,281 to 6,418 rpmH SO0008: dnaA - chromosomal replication initiator protein DnaA (NCBI ptt file), at 6,873 to 8,255 dnaA SO0009: dnaN - DNA polymerase III, beta subunit (NCBI ptt file), at 8,276 to 9,376 dnaN Position (kb) 6 7 8 9Strain fitness (log2 ratio) -1 0 1at 5.901 kb on - strand, within SO0005at 6.598 kb on + strandat 6.621 kb on + strandat 6.651 kb on + strandat 6.659 kb on - strandat 6.659 kb on - strandat 6.659 kb on - strandat 6.756 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol%
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5,901 - SO0005 0.84 +0.1
6,598 + +0.2
6,621 + +0.2
6,651 + -0.2
6,659 - -1.1
6,659 - +0.4
6,659 - +0.4
6,756 + +0.1

Or see this region's nucleotide sequence