Experiment: Minimal media, pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_R0029 and Sama_0363 are separated by 129 nucleotides Sama_0363 and Sama_0364 are separated by 41 nucleotides Sama_0364 and Sama_0365 are separated by 61 nucleotides Sama_0365 and coaE overlap by 8 nucleotides coaE and Sama_0367 overlap by 1 nucleotides
Sama_R0029: Sama_R0029 - 5S ribosomal RNA (RefSeq), at 441,632 to 441,746
_R0029
Sama_0363: Sama_0363 - mutator MutT protein (RefSeq), at 441,876 to 442,271
_0363
Sama_0364: Sama_0364 - hypothetical protein (RefSeq), at 442,313 to 442,528
_0364
Sama_0365: Sama_0365 - hypothetical protein (RefSeq), at 442,590 to 443,324
_0365
Sama_0366: coaE - dephospho-CoA kinase (RefSeq), at 443,317 to 443,934
coaE
Sama_0367: Sama_0367 - prepilin peptidase (RefSeq), at 443,934 to 444,848
_0367
Position (kb) 442
443
444 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 441.591 kb on + strand at 441.591 kb on + strand at 441.608 kb on + strand at 441.608 kb on + strand at 441.679 kb on + strand, within Sama_R0029 at 441.679 kb on + strand, within Sama_R0029 at 441.704 kb on + strand, within Sama_R0029 at 441.704 kb on + strand, within Sama_R0029 at 441.704 kb on + strand, within Sama_R0029 at 441.730 kb on + strand, within Sama_R0029 at 441.730 kb on + strand, within Sama_R0029 at 441.788 kb on + strand at 441.819 kb on + strand at 441.831 kb on - strand at 441.846 kb on + strand at 441.846 kb on + strand at 441.881 kb on + strand at 441.881 kb on + strand at 441.882 kb on - strand at 441.882 kb on - strand at 441.882 kb on - strand at 441.899 kb on + strand at 441.899 kb on + strand at 441.935 kb on - strand, within Sama_0363 at 441.935 kb on - strand, within Sama_0363 at 442.022 kb on + strand, within Sama_0363 at 442.023 kb on - strand, within Sama_0363 at 442.023 kb on - strand, within Sama_0363 at 442.032 kb on + strand, within Sama_0363 at 442.032 kb on + strand, within Sama_0363 at 442.032 kb on + strand, within Sama_0363 at 442.033 kb on - strand, within Sama_0363 at 442.033 kb on - strand, within Sama_0363 at 442.033 kb on - strand, within Sama_0363 at 442.049 kb on - strand, within Sama_0363 at 442.067 kb on + strand, within Sama_0363 at 442.067 kb on + strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.111 kb on - strand, within Sama_0363 at 442.235 kb on + strand at 442.235 kb on + strand at 442.236 kb on - strand at 442.236 kb on - strand at 442.236 kb on - strand at 442.236 kb on - strand at 442.246 kb on - strand at 442.265 kb on + strand at 442.265 kb on + strand at 442.265 kb on + strand at 442.265 kb on + strand at 442.265 kb on + strand at 442.266 kb on - strand at 442.266 kb on - strand at 442.266 kb on - strand at 442.271 kb on + strand at 442.271 kb on + strand at 442.271 kb on + strand at 442.271 kb on + strand at 442.271 kb on + strand at 442.272 kb on - strand at 442.272 kb on - strand at 442.287 kb on + strand at 442.288 kb on - strand at 442.288 kb on - strand at 442.290 kb on + strand at 442.290 kb on + strand at 442.290 kb on + strand at 442.290 kb on + strand at 442.291 kb on - strand at 442.327 kb on + strand at 442.327 kb on + strand at 442.327 kb on + strand at 442.327 kb on + strand at 442.388 kb on + strand, within Sama_0364 at 442.425 kb on - strand, within Sama_0364 at 442.425 kb on - strand, within Sama_0364 at 442.487 kb on - strand, within Sama_0364 at 442.514 kb on - strand at 442.514 kb on - strand at 442.529 kb on - strand at 442.529 kb on - strand at 442.551 kb on + strand at 442.551 kb on + strand at 442.551 kb on + strand at 442.552 kb on - strand at 442.552 kb on - strand at 442.552 kb on - strand at 442.552 kb on - strand at 442.577 kb on + strand at 442.578 kb on - strand at 442.578 kb on - strand at 442.582 kb on + strand at 442.583 kb on - strand at 442.583 kb on - strand at 442.583 kb on - strand at 442.611 kb on - strand at 442.611 kb on - strand at 442.625 kb on + strand at 442.688 kb on + strand, within Sama_0365 at 442.765 kb on + strand, within Sama_0365 at 442.765 kb on + strand, within Sama_0365 at 442.766 kb on - strand, within Sama_0365 at 442.795 kb on + strand, within Sama_0365 at 442.841 kb on + strand, within Sama_0365 at 442.842 kb on - strand, within Sama_0365 at 442.876 kb on + strand, within Sama_0365 at 442.876 kb on + strand, within Sama_0365 at 442.876 kb on + strand, within Sama_0365 at 442.877 kb on - strand, within Sama_0365 at 442.930 kb on - strand, within Sama_0365 at 442.940 kb on + strand, within Sama_0365 at 442.940 kb on + strand, within Sama_0365 at 442.975 kb on - strand, within Sama_0365 at 442.975 kb on - strand, within Sama_0365 at 442.984 kb on - strand, within Sama_0365 at 442.992 kb on - strand, within Sama_0365 at 443.048 kb on + strand, within Sama_0365 at 443.048 kb on + strand, within Sama_0365 at 443.049 kb on - strand, within Sama_0365 at 443.049 kb on - strand, within Sama_0365 at 443.052 kb on + strand, within Sama_0365 at 443.053 kb on - strand, within Sama_0365 at 443.097 kb on + strand, within Sama_0365 at 443.097 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.121 kb on - strand, within Sama_0365 at 443.121 kb on - strand, within Sama_0365 at 443.136 kb on + strand, within Sama_0365 at 443.162 kb on - strand, within Sama_0365 at 443.178 kb on + strand, within Sama_0365 at 443.178 kb on + strand, within Sama_0365 at 443.188 kb on - strand, within Sama_0365 at 443.196 kb on + strand, within Sama_0365 at 443.196 kb on + strand, within Sama_0365 at 443.196 kb on + strand, within Sama_0365 at 443.197 kb on - strand, within Sama_0365 at 443.237 kb on + strand, within Sama_0365 at 443.237 kb on + strand, within Sama_0365 at 443.237 kb on + strand, within Sama_0365 at 443.237 kb on + strand, within Sama_0365 at 443.238 kb on - strand, within Sama_0365 at 443.238 kb on - strand, within Sama_0365 at 443.308 kb on + strand at 443.308 kb on + strand at 443.308 kb on + strand at 443.308 kb on + strand at 443.308 kb on + strand at 443.308 kb on + strand at 443.308 kb on + strand at 443.309 kb on - strand at 443.309 kb on - strand at 443.310 kb on + strand at 443.310 kb on + strand at 443.310 kb on + strand at 443.310 kb on + strand at 443.310 kb on + strand at 443.311 kb on - strand at 443.350 kb on + strand at 443.923 kb on - strand at 443.964 kb on - strand at 443.979 kb on - strand at 443.979 kb on - strand at 444.008 kb on - strand at 444.039 kb on - strand, within Sama_0367 at 444.064 kb on - strand, within Sama_0367 at 444.102 kb on - strand, within Sama_0367
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media, pH7 remove 441,591 + +0.6 441,591 + -0.2 441,608 + +0.9 441,608 + +0.4 441,679 + Sama_R0029 0.41 +0.3 441,679 + Sama_R0029 0.41 +1.0 441,704 + Sama_R0029 0.63 -1.3 441,704 + Sama_R0029 0.63 -0.2 441,704 + Sama_R0029 0.63 -0.7 441,730 + Sama_R0029 0.85 +0.8 441,730 + Sama_R0029 0.85 +0.0 441,788 + +0.1 441,819 + +0.2 441,831 - -0.2 441,846 + -0.0 441,846 + +1.0 441,881 + -1.3 441,881 + -2.8 441,882 - -1.8 441,882 - -0.1 441,882 - +0.1 441,899 + +0.1 441,899 + -0.1 441,935 - Sama_0363 0.15 -1.8 441,935 - Sama_0363 0.15 -0.5 442,022 + Sama_0363 0.37 -0.9 442,023 - Sama_0363 0.37 +0.6 442,023 - Sama_0363 0.37 +1.8 442,032 + Sama_0363 0.39 -0.5 442,032 + Sama_0363 0.39 +1.1 442,032 + Sama_0363 0.39 +0.1 442,033 - Sama_0363 0.40 -0.3 442,033 - Sama_0363 0.40 -0.3 442,033 - Sama_0363 0.40 +0.4 442,049 - Sama_0363 0.44 -0.1 442,067 + Sama_0363 0.48 -2.7 442,067 + Sama_0363 0.48 -0.4 442,068 - Sama_0363 0.48 -1.9 442,068 - Sama_0363 0.48 -2.2 442,068 - Sama_0363 0.48 +0.1 442,068 - Sama_0363 0.48 -1.6 442,068 - Sama_0363 0.48 -0.9 442,069 + Sama_0363 0.49 +0.6 442,069 + Sama_0363 0.49 +0.0 442,069 + Sama_0363 0.49 +0.4 442,069 + Sama_0363 0.49 -2.2 442,069 + Sama_0363 0.49 +1.3 442,069 + Sama_0363 0.49 +0.0 442,069 + Sama_0363 0.49 +1.3 442,070 - Sama_0363 0.49 -0.3 442,070 - Sama_0363 0.49 -0.7 442,070 - Sama_0363 0.49 -0.1 442,070 - Sama_0363 0.49 +0.6 442,070 - Sama_0363 0.49 -0.2 442,070 - Sama_0363 0.49 +0.4 442,070 - Sama_0363 0.49 -1.0 442,070 - Sama_0363 0.49 -1.9 442,070 - Sama_0363 0.49 -0.1 442,070 - Sama_0363 0.49 -0.3 442,070 - Sama_0363 0.49 -0.2 442,070 - Sama_0363 0.49 -0.9 442,070 - Sama_0363 0.49 +0.3 442,111 - Sama_0363 0.59 +1.4 442,235 + +0.4 442,235 + +0.9 442,236 - +0.2 442,236 - -0.2 442,236 - +0.3 442,236 - +0.4 442,246 - +0.1 442,265 + +1.0 442,265 + -1.9 442,265 + -0.1 442,265 + -1.8 442,265 + -1.3 442,266 - +1.4 442,266 - -0.9 442,266 - -0.2 442,271 + -0.4 442,271 + -0.1 442,271 + +1.3 442,271 + -0.6 442,271 + -1.3 442,272 - -0.1 442,272 - -0.4 442,287 + +0.2 442,288 - +0.4 442,288 - -0.9 442,290 + -1.2 442,290 + -0.6 442,290 + -1.6 442,290 + +1.4 442,291 - +1.8 442,327 + -0.7 442,327 + +0.2 442,327 + -0.7 442,327 + -0.2 442,388 + Sama_0364 0.35 -2.5 442,425 - Sama_0364 0.52 -1.3 442,425 - Sama_0364 0.52 -2.6 442,487 - Sama_0364 0.81 -3.1 442,514 - -1.8 442,514 - +0.0 442,529 - -1.6 442,529 - -0.3 442,551 + +0.3 442,551 + -2.1 442,551 + +1.4 442,552 - +0.8 442,552 - -1.9 442,552 - -1.9 442,552 - -1.2 442,577 + +0.0 442,578 - +0.4 442,578 - -0.8 442,582 + -1.4 442,583 - -0.7 442,583 - -1.9 442,583 - +0.3 442,611 - -0.2 442,611 - -0.8 442,625 + -1.4 442,688 + Sama_0365 0.13 +1.3 442,765 + Sama_0365 0.24 +0.6 442,765 + Sama_0365 0.24 +0.7 442,766 - Sama_0365 0.24 -1.9 442,795 + Sama_0365 0.28 -2.4 442,841 + Sama_0365 0.34 +0.4 442,842 - Sama_0365 0.34 -0.3 442,876 + Sama_0365 0.39 -2.1 442,876 + Sama_0365 0.39 -0.5 442,876 + Sama_0365 0.39 +1.1 442,877 - Sama_0365 0.39 -1.4 442,930 - Sama_0365 0.46 -1.9 442,940 + Sama_0365 0.48 -0.6 442,940 + Sama_0365 0.48 -0.2 442,975 - Sama_0365 0.52 -0.1 442,975 - Sama_0365 0.52 -2.1 442,984 - Sama_0365 0.54 -0.9 442,992 - Sama_0365 0.55 -0.8 443,048 + Sama_0365 0.62 -4.2 443,048 + Sama_0365 0.62 -1.8 443,049 - Sama_0365 0.62 -1.8 443,049 - Sama_0365 0.62 -1.1 443,052 + Sama_0365 0.63 +0.8 443,053 - Sama_0365 0.63 -1.2 443,097 + Sama_0365 0.69 +0.9 443,097 + Sama_0365 0.69 -0.0 443,100 + Sama_0365 0.69 -0.4 443,100 + Sama_0365 0.69 +0.4 443,100 + Sama_0365 0.69 +1.0 443,100 + Sama_0365 0.69 +0.2 443,100 + Sama_0365 0.69 +1.0 443,100 + Sama_0365 0.69 +2.0 443,100 + Sama_0365 0.69 +1.1 443,100 + Sama_0365 0.69 -0.8 443,100 + Sama_0365 0.69 -1.4 443,101 - Sama_0365 0.70 +0.0 443,101 - Sama_0365 0.70 -1.3 443,101 - Sama_0365 0.70 +0.3 443,101 - Sama_0365 0.70 -0.3 443,101 - Sama_0365 0.70 -0.2 443,101 - Sama_0365 0.70 +0.7 443,101 - Sama_0365 0.70 -0.2 443,101 - Sama_0365 0.70 -0.0 443,101 - Sama_0365 0.70 +0.3 443,101 - Sama_0365 0.70 -0.9 443,121 - Sama_0365 0.72 +1.1 443,121 - Sama_0365 0.72 -1.4 443,136 + Sama_0365 0.74 +2.5 443,162 - Sama_0365 0.78 +0.8 443,178 + Sama_0365 0.80 -0.3 443,178 + Sama_0365 0.80 -1.3 443,188 - Sama_0365 0.81 +1.0 443,196 + Sama_0365 0.82 -1.6 443,196 + Sama_0365 0.82 -0.4 443,196 + Sama_0365 0.82 +0.8 443,197 - Sama_0365 0.83 -1.9 443,237 + Sama_0365 0.88 +0.6 443,237 + Sama_0365 0.88 -0.1 443,237 + Sama_0365 0.88 +1.1 443,237 + Sama_0365 0.88 +1.1 443,238 - Sama_0365 0.88 -1.2 443,238 - Sama_0365 0.88 +0.1 443,308 + -0.3 443,308 + -0.6 443,308 + -1.9 443,308 + +0.9 443,308 + +0.4 443,308 + -1.0 443,308 + -1.3 443,309 - -0.8 443,309 - -1.4 443,310 + +0.4 443,310 + -1.6 443,310 + -0.9 443,310 + -0.4 443,310 + +1.8 443,311 - -0.8 443,350 + -1.7 443,923 - -2.6 443,964 - -1.4 443,979 - -1.2 443,979 - -1.8 444,008 - -1.4 444,039 - Sama_0367 0.11 -1.6 444,064 - Sama_0367 0.14 -1.6 444,102 - Sama_0367 0.18 -1.2
Or see this region's nucleotide sequence