Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1890 and SO1891 are separated by 706 nucleotides SO1891 and atoD are separated by 106 nucleotides atoD and mvaB are separated by 81 nucleotides
SO1890: SO1890 - conserved hypothetical protein (NCBI ptt file), at 1,986,246 to 1,987,223
SO1890
SO1891: SO1891 - 3-oxoadipate CoA-succinyl transferase, beta subunit (NCBI ptt file), at 1,987,930 to 1,988,586
SO1891
SO1892: atoD - acetate CoA-transferase, subunit A (NCBI ptt file), at 1,988,693 to 1,989,397
atoD
SO1893: mvaB - hydroxymethylglutaryl-CoA lyase (NCBI ptt file), at 1,989,479 to 1,990,426
mvaB
Position (kb)
1987
1988
1989 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1987.267 kb on - strand at 1987.350 kb on - strand at 1987.821 kb on - strand at 1988.059 kb on + strand, within SO1891 at 1988.067 kb on - strand, within SO1891 at 1988.127 kb on + strand, within SO1891 at 1988.135 kb on - strand, within SO1891 at 1988.140 kb on - strand, within SO1891 at 1988.221 kb on - strand, within SO1891 at 1988.221 kb on - strand, within SO1891 at 1988.221 kb on - strand, within SO1891 at 1989.017 kb on + strand, within atoD at 1989.017 kb on + strand, within atoD at 1989.025 kb on - strand, within atoD at 1989.125 kb on - strand, within atoD at 1989.133 kb on + strand, within atoD at 1989.206 kb on - strand, within atoD
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 1,987,267 - -1.5 1,987,350 - +0.1 1,987,821 - -0.4 1,988,059 + SO1891 0.20 -2.8 1,988,067 - SO1891 0.21 -2.9 1,988,127 + SO1891 0.30 -1.5 1,988,135 - SO1891 0.31 -2.1 1,988,140 - SO1891 0.32 -2.9 1,988,221 - SO1891 0.44 -3.0 1,988,221 - SO1891 0.44 -2.0 1,988,221 - SO1891 0.44 -1.7 1,989,017 + atoD SO1892 0.46 -3.8 1,989,017 + atoD SO1892 0.46 -1.5 1,989,025 - atoD SO1892 0.47 -3.1 1,989,125 - atoD SO1892 0.61 -1.1 1,989,133 + atoD SO1892 0.62 -1.8 1,989,206 - atoD SO1892 0.73 -1.2
Or see this region's nucleotide sequence