Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ccmA and scyA are separated by 325 nucleotides scyA and SO0265 are separated by 80 nucleotides SO0265 and ccmF-1 are separated by 398 nucleotides
SO0263: ccmA - heme exporter protein CcmA (NCBI ptt file), at 262,669 to 263,319
ccmA
SO0264: scyA - cytochrome c (NCBI ptt file), at 263,645 to 263,944
scyA
SO0265: SO0265 - conserved hypothetical protein (NCBI ptt file), at 264,025 to 265,272
SO0265
SO0266: ccmF-1 - cytochrome c-type biogenesis protein CcmF (NCBI ptt file), at 265,671 to 267,650
ccmF-1
Position (kb) 264
265
266 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 263.041 kb on - strand, within ccmA at 263.120 kb on - strand, within ccmA at 263.120 kb on - strand, within ccmA at 263.267 kb on + strand at 263.290 kb on - strand at 263.382 kb on + strand at 263.397 kb on + strand at 263.406 kb on - strand at 263.406 kb on - strand at 263.406 kb on - strand at 263.430 kb on - strand at 263.431 kb on - strand at 263.499 kb on + strand at 263.509 kb on + strand at 263.582 kb on + strand at 263.587 kb on - strand at 263.593 kb on + strand at 263.613 kb on - strand at 263.636 kb on + strand at 263.659 kb on + strand at 263.734 kb on + strand, within scyA at 263.739 kb on + strand, within scyA at 263.752 kb on + strand, within scyA at 263.785 kb on + strand, within scyA at 263.937 kb on - strand at 264.094 kb on + strand at 264.147 kb on + strand at 264.147 kb on + strand at 264.197 kb on - strand, within SO0265 at 264.221 kb on + strand, within SO0265 at 264.246 kb on - strand, within SO0265 at 264.246 kb on - strand, within SO0265 at 264.327 kb on + strand, within SO0265 at 264.403 kb on - strand, within SO0265 at 264.405 kb on + strand, within SO0265 at 264.413 kb on - strand, within SO0265 at 264.413 kb on - strand, within SO0265 at 264.438 kb on - strand, within SO0265 at 264.454 kb on + strand, within SO0265 at 264.454 kb on + strand, within SO0265 at 264.469 kb on + strand, within SO0265 at 264.477 kb on - strand, within SO0265 at 264.477 kb on - strand, within SO0265 at 264.477 kb on - strand, within SO0265 at 264.477 kb on - strand, within SO0265 at 264.477 kb on - strand, within SO0265 at 264.477 kb on - strand, within SO0265 at 264.615 kb on - strand, within SO0265 at 264.666 kb on + strand, within SO0265 at 264.674 kb on - strand, within SO0265 at 264.676 kb on + strand, within SO0265 at 264.676 kb on + strand, within SO0265 at 264.684 kb on - strand, within SO0265 at 264.774 kb on - strand, within SO0265 at 264.864 kb on - strand, within SO0265 at 264.977 kb on - strand, within SO0265 at 264.994 kb on - strand, within SO0265 at 265.038 kb on - strand, within SO0265 at 265.095 kb on - strand, within SO0265 at 265.211 kb on + strand at 265.221 kb on + strand at 265.221 kb on + strand at 265.221 kb on + strand at 265.221 kb on + strand at 265.221 kb on + strand at 265.229 kb on - strand at 265.229 kb on - strand at 265.229 kb on - strand at 265.229 kb on - strand at 265.358 kb on - strand at 265.721 kb on - strand at 265.772 kb on - strand at 265.799 kb on - strand at 265.942 kb on + strand, within ccmF-1 at 265.956 kb on + strand, within ccmF-1 at 266.081 kb on - strand, within ccmF-1 at 266.098 kb on + strand, within ccmF-1 at 266.098 kb on + strand, within ccmF-1 at 266.098 kb on + strand, within ccmF-1 at 266.098 kb on + strand, within ccmF-1 at 266.106 kb on - strand, within ccmF-1 at 266.106 kb on - strand, within ccmF-1 at 266.195 kb on - strand, within ccmF-1 at 266.255 kb on - strand, within ccmF-1
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 263,041 - ccmA SO0263 0.57 -1.8 263,120 - ccmA SO0263 0.69 -0.5 263,120 - ccmA SO0263 0.69 -2.1 263,267 + -1.1 263,290 - -1.6 263,382 + -1.3 263,397 + +0.1 263,406 - -0.3 263,406 - -0.3 263,406 - -0.2 263,430 - -0.3 263,431 - +0.7 263,499 + -1.7 263,509 + -0.7 263,582 + -1.8 263,587 - +0.9 263,593 + -2.6 263,613 - -3.3 263,636 + -2.6 263,659 + -3.6 263,734 + scyA SO0264 0.30 -2.1 263,739 + scyA SO0264 0.31 -1.1 263,752 + scyA SO0264 0.36 -0.7 263,785 + scyA SO0264 0.47 +1.1 263,937 - -1.1 264,094 + +0.1 264,147 + +1.1 264,147 + -1.1 264,197 - SO0265 0.14 +0.6 264,221 + SO0265 0.16 -0.2 264,246 - SO0265 0.18 -0.2 264,246 - SO0265 0.18 -0.4 264,327 + SO0265 0.24 +0.6 264,403 - SO0265 0.30 +0.5 264,405 + SO0265 0.30 +0.6 264,413 - SO0265 0.31 +0.1 264,413 - SO0265 0.31 -1.1 264,438 - SO0265 0.33 -0.1 264,454 + SO0265 0.34 -1.2 264,454 + SO0265 0.34 -1.4 264,469 + SO0265 0.36 -0.3 264,477 - SO0265 0.36 -4.5 264,477 - SO0265 0.36 -3.0 264,477 - SO0265 0.36 -2.6 264,477 - SO0265 0.36 -4.0 264,477 - SO0265 0.36 -2.0 264,477 - SO0265 0.36 -2.3 264,615 - SO0265 0.47 -2.9 264,666 + SO0265 0.51 +1.8 264,674 - SO0265 0.52 -0.9 264,676 + SO0265 0.52 -2.2 264,676 + SO0265 0.52 -3.5 264,684 - SO0265 0.53 -4.1 264,774 - SO0265 0.60 -2.8 264,864 - SO0265 0.67 -2.8 264,977 - SO0265 0.76 -3.3 264,994 - SO0265 0.78 -0.2 265,038 - SO0265 0.81 -1.2 265,095 - SO0265 0.86 -1.2 265,211 + -1.6 265,221 + -0.3 265,221 + -0.2 265,221 + -0.7 265,221 + -3.1 265,221 + -1.2 265,229 - -2.5 265,229 - -1.9 265,229 - -3.3 265,229 - -2.6 265,358 - +0.8 265,721 - -0.5 265,772 - -1.5 265,799 - -2.2 265,942 + ccmF-1 SO0266 0.14 -1.9 265,956 + ccmF-1 SO0266 0.14 -1.6 266,081 - ccmF-1 SO0266 0.21 -2.8 266,098 + ccmF-1 SO0266 0.22 -2.0 266,098 + ccmF-1 SO0266 0.22 -2.2 266,098 + ccmF-1 SO0266 0.22 -1.3 266,098 + ccmF-1 SO0266 0.22 -2.3 266,106 - ccmF-1 SO0266 0.22 -0.7 266,106 - ccmF-1 SO0266 0.22 -2.0 266,195 - ccmF-1 SO0266 0.26 -2.4 266,255 - ccmF-1 SO0266 0.29 -1.5
Or see this region's nucleotide sequence