Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioC and bioF are separated by 120 nucleotides bioF and SO2739.1 are separated by 29 nucleotides SO2739.1 and bioB overlap by 6 nucleotides
SO2738: bioC - biotin synthesis protein BioC (NCBI ptt file), at 2,853,336 to 2,854,163
bioC
SO2739: bioF - 8-amino-7-oxononanoate synthase (NCBI ptt file), at 2,854,284 to 2,855,489
bioF
SO2739.1: SO2739.1 - hypothetical protein (NCBI ptt file), at 2,855,519 to 2,855,716
SO2739.1
SO2740: bioB - biotin synthase (NCBI ptt file), at 2,855,711 to 2,856,763
bioB
Position (kb)
2854
2855
2856 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 2853.392 kb on - strand at 2853.393 kb on - strand at 2853.845 kb on - strand, within bioC at 2853.885 kb on - strand, within bioC at 2853.980 kb on - strand, within bioC at 2854.231 kb on - strand at 2854.277 kb on - strand at 2854.637 kb on - strand, within bioF at 2854.637 kb on - strand, within bioF at 2854.732 kb on - strand, within bioF at 2854.766 kb on - strand, within bioF at 2854.999 kb on - strand, within bioF at 2855.110 kb on - strand, within bioF at 2855.112 kb on - strand, within bioF at 2855.311 kb on - strand, within bioF at 2855.426 kb on - strand at 2855.436 kb on - strand at 2855.539 kb on - strand, within SO2739.1 at 2855.580 kb on + strand, within SO2739.1 at 2855.711 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 2,853,392 - +3.0 2,853,393 - +2.2 2,853,845 - bioC SO2738 0.61 -2.8 2,853,885 - bioC SO2738 0.66 -0.0 2,853,980 - bioC SO2738 0.78 +2.7 2,854,231 - +1.2 2,854,277 - -2.2 2,854,637 - bioF SO2739 0.29 +3.4 2,854,637 - bioF SO2739 0.29 +3.0 2,854,732 - bioF SO2739 0.37 +3.4 2,854,766 - bioF SO2739 0.40 +5.7 2,854,999 - bioF SO2739 0.59 +5.8 2,855,110 - bioF SO2739 0.68 +5.2 2,855,112 - bioF SO2739 0.69 +5.3 2,855,311 - bioF SO2739 0.85 +6.3 2,855,426 - +6.5 2,855,436 - +2.3 2,855,539 - SO2739.1 0.10 -0.0 2,855,580 + SO2739.1 0.31 +0.3 2,855,711 + -2.0
Or see this region's nucleotide sequence