Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2219 and SO2220 are separated by 17 nucleotides SO2220 and pabB are separated by 27 nucleotides pabB and SO2222 are separated by 122 nucleotides
SO2219: SO2219 - hypothetical protein (NCBI ptt file), at 2,334,538 to 2,334,879
SO2219
SO2220: SO2220 - MutT/nudix family protein (NCBI ptt file), at 2,334,897 to 2,335,484
SO2220
SO2221: pabB - para-aminobenzoate synthase, component I (NCBI ptt file), at 2,335,512 to 2,336,915
pabB
SO2222: SO2222 - fumarate hydratase, class I, anaerobic, putative (NCBI ptt file), at 2,337,038 to 2,338,588
SO2222
Position (kb)
2335
2336
2337 Strain fitness (log2 ratio)
-1
0
1
2 at 2334.638 kb on - strand, within SO2219 at 2334.644 kb on - strand, within SO2219 at 2334.685 kb on - strand, within SO2219 at 2334.789 kb on - strand, within SO2219 at 2334.913 kb on + strand at 2334.918 kb on + strand at 2335.000 kb on + strand, within SO2220 at 2335.013 kb on - strand, within SO2220 at 2335.257 kb on - strand, within SO2220 at 2335.378 kb on + strand, within SO2220 at 2335.438 kb on + strand at 2335.438 kb on + strand at 2335.446 kb on - strand at 2335.618 kb on + strand at 2335.689 kb on + strand, within pabB at 2335.899 kb on - strand, within pabB at 2336.217 kb on - strand, within pabB at 2336.321 kb on + strand, within pabB at 2336.550 kb on + strand, within pabB at 2336.558 kb on - strand, within pabB at 2336.580 kb on - strand, within pabB at 2336.587 kb on + strand, within pabB at 2336.597 kb on + strand, within pabB at 2336.610 kb on + strand, within pabB at 2336.708 kb on - strand, within pabB at 2336.821 kb on - strand at 2336.849 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 2,334,638 - SO2219 0.29 -0.2 2,334,644 - SO2219 0.31 -0.9 2,334,685 - SO2219 0.43 +0.8 2,334,789 - SO2219 0.73 -1.2 2,334,913 + +1.5 2,334,918 + -1.5 2,335,000 + SO2220 0.18 +1.2 2,335,013 - SO2220 0.20 +0.9 2,335,257 - SO2220 0.61 +1.1 2,335,378 + SO2220 0.82 -0.9 2,335,438 + -0.1 2,335,438 + +0.3 2,335,446 - +1.1 2,335,618 + +1.3 2,335,689 + pabB SO2221 0.13 +2.6 2,335,899 - pabB SO2221 0.28 +0.1 2,336,217 - pabB SO2221 0.50 +0.6 2,336,321 + pabB SO2221 0.58 +0.8 2,336,550 + pabB SO2221 0.74 +0.9 2,336,558 - pabB SO2221 0.75 +0.7 2,336,580 - pabB SO2221 0.76 +1.2 2,336,587 + pabB SO2221 0.77 +0.4 2,336,597 + pabB SO2221 0.77 +0.3 2,336,610 + pabB SO2221 0.78 -1.7 2,336,708 - pabB SO2221 0.85 -0.5 2,336,821 - -1.3 2,336,849 + +0.1
Or see this region's nucleotide sequence