Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgsA and tRNA-Gly-5 are separated by 143 nucleotides tRNA-Gly-5 and tRNA-Cys-1 are separated by 21 nucleotides tRNA-Cys-1 and tRNA-Leu-2 are separated by 48 nucleotides tRNA-Leu-2 and tRNA-Leu-1 are separated by 132 nucleotides tRNA-Leu-1 and SO1863 are separated by 315 nucleotides SO1863 and SO1864 are separated by 190 nucleotides SO1864 and SO1865 are separated by 44 nucleotides
SO1862: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (NCBI ptt file), at 1,954,433 to 1,954,981
pgsA
SO_t033: tRNA-Gly-5 - tRNA-Gly (RefSeq), at 1,955,125 to 1,955,200
tRNA-Gly-5
SO_t034: tRNA-Cys-1 - tRNA-Cys (RefSeq), at 1,955,222 to 1,955,295
tRNA-Cys-1
SO_t035: tRNA-Leu-2 - tRNA-Leu (RefSeq), at 1,955,344 to 1,955,430
tRNA-Leu-2
SO_t036: tRNA-Leu-1 - tRNA-Leu (RefSeq), at 1,955,563 to 1,955,649
tRNA-Leu-1
SO1863: SO1863 - DNA-binding protein, HU family (NCBI ptt file), at 1,955,965 to 1,956,240
SO1863
SO1864: SO1864 - hypothetical protein (NCBI ptt file), at 1,956,431 to 1,957,150
SO1864
SO1865: SO1865 - ABC transporter, ATP-binding protein (NCBI ptt file), at 1,957,195 to 1,958,103
SO1865
Position (kb)
1955
1956
1957 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1955.397 kb on + strand, within tRNA-Leu-2 at 1955.448 kb on + strand at 1955.465 kb on + strand at 1955.465 kb on + strand at 1955.647 kb on + strand at 1955.778 kb on + strand at 1955.786 kb on - strand at 1955.786 kb on - strand at 1955.798 kb on + strand at 1955.822 kb on + strand at 1955.855 kb on - strand at 1955.879 kb on - strand at 1955.928 kb on + strand at 1955.983 kb on + strand at 1955.991 kb on - strand at 1955.993 kb on + strand, within SO1863 at 1956.008 kb on - strand, within SO1863 at 1956.150 kb on + strand, within SO1863 at 1956.174 kb on + strand, within SO1863 at 1956.179 kb on + strand, within SO1863 at 1956.181 kb on - strand, within SO1863 at 1956.182 kb on - strand, within SO1863 at 1956.185 kb on + strand, within SO1863 at 1956.185 kb on + strand, within SO1863 at 1956.185 kb on + strand, within SO1863 at 1956.193 kb on - strand, within SO1863 at 1956.193 kb on - strand, within SO1863 at 1956.193 kb on - strand, within SO1863 at 1956.195 kb on + strand, within SO1863 at 1956.195 kb on + strand, within SO1863 at 1956.225 kb on + strand at 1956.228 kb on - strand at 1956.233 kb on - strand at 1956.233 kb on - strand at 1956.233 kb on - strand at 1956.272 kb on + strand at 1956.277 kb on + strand at 1956.298 kb on - strand at 1956.316 kb on - strand at 1956.327 kb on + strand at 1956.374 kb on - strand at 1956.379 kb on + strand at 1956.384 kb on + strand at 1956.397 kb on - strand at 1956.400 kb on + strand at 1956.400 kb on + strand at 1956.400 kb on + strand at 1956.400 kb on + strand at 1956.400 kb on + strand at 1956.400 kb on + strand at 1956.407 kb on - strand at 1956.407 kb on - strand at 1956.407 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.408 kb on - strand at 1956.445 kb on + strand at 1956.445 kb on + strand at 1956.498 kb on - strand at 1956.531 kb on + strand, within SO1864 at 1956.539 kb on + strand, within SO1864 at 1956.615 kb on + strand, within SO1864 at 1956.657 kb on - strand, within SO1864 at 1956.659 kb on + strand, within SO1864 at 1956.659 kb on + strand, within SO1864 at 1956.659 kb on + strand, within SO1864 at 1956.659 kb on + strand, within SO1864 at 1956.659 kb on + strand, within SO1864 at 1956.659 kb on + strand, within SO1864 at 1956.667 kb on - strand, within SO1864 at 1956.696 kb on + strand, within SO1864 at 1956.696 kb on + strand, within SO1864 at 1956.696 kb on + strand, within SO1864 at 1956.696 kb on + strand, within SO1864 at 1956.696 kb on + strand, within SO1864 at 1956.696 kb on + strand, within SO1864 at 1956.696 kb on + strand, within SO1864 at 1956.696 kb on + strand, within SO1864 at 1956.696 kb on + strand, within SO1864 at 1956.697 kb on + strand, within SO1864 at 1956.704 kb on - strand, within SO1864 at 1956.704 kb on - strand, within SO1864 at 1956.704 kb on - strand, within SO1864 at 1956.704 kb on - strand, within SO1864 at 1956.704 kb on - strand, within SO1864 at 1956.704 kb on - strand, within SO1864 at 1956.704 kb on - strand, within SO1864 at 1956.705 kb on - strand, within SO1864 at 1956.705 kb on - strand, within SO1864 at 1956.705 kb on - strand, within SO1864 at 1956.705 kb on - strand, within SO1864 at 1956.761 kb on - strand, within SO1864 at 1956.763 kb on + strand, within SO1864 at 1956.763 kb on + strand, within SO1864 at 1956.763 kb on + strand, within SO1864 at 1956.771 kb on - strand, within SO1864 at 1956.771 kb on - strand, within SO1864 at 1956.771 kb on - strand, within SO1864 at 1956.817 kb on - strand, within SO1864 at 1956.841 kb on - strand, within SO1864 at 1956.845 kb on + strand, within SO1864 at 1956.870 kb on - strand, within SO1864 at 1956.870 kb on - strand, within SO1864 at 1956.878 kb on - strand, within SO1864 at 1956.883 kb on - strand, within SO1864 at 1956.900 kb on - strand, within SO1864 at 1956.921 kb on + strand, within SO1864 at 1956.945 kb on - strand, within SO1864 at 1956.945 kb on - strand, within SO1864 at 1956.953 kb on + strand, within SO1864 at 1956.958 kb on + strand, within SO1864 at 1956.958 kb on + strand, within SO1864 at 1956.958 kb on + strand, within SO1864 at 1956.958 kb on + strand, within SO1864 at 1956.965 kb on + strand, within SO1864 at 1956.966 kb on - strand, within SO1864 at 1956.966 kb on - strand, within SO1864 at 1956.966 kb on - strand, within SO1864 at 1956.970 kb on + strand, within SO1864 at 1956.970 kb on + strand, within SO1864 at 1956.970 kb on + strand, within SO1864 at 1956.970 kb on + strand, within SO1864 at 1956.971 kb on - strand, within SO1864 at 1956.973 kb on - strand, within SO1864 at 1956.973 kb on - strand, within SO1864 at 1956.973 kb on - strand, within SO1864 at 1956.975 kb on + strand, within SO1864 at 1956.978 kb on - strand, within SO1864 at 1956.978 kb on - strand, within SO1864 at 1956.978 kb on - strand, within SO1864 at 1956.983 kb on - strand, within SO1864 at 1956.988 kb on + strand, within SO1864 at 1956.995 kb on - strand, within SO1864 at 1957.006 kb on + strand, within SO1864 at 1957.006 kb on + strand, within SO1864 at 1957.006 kb on + strand, within SO1864 at 1957.006 kb on + strand, within SO1864 at 1957.006 kb on + strand, within SO1864 at 1957.007 kb on + strand, within SO1864 at 1957.014 kb on + strand, within SO1864 at 1957.022 kb on - strand, within SO1864 at 1957.023 kb on - strand, within SO1864 at 1957.055 kb on - strand, within SO1864 at 1957.107 kb on + strand at 1957.107 kb on - strand at 1957.115 kb on + strand at 1957.127 kb on + strand at 1957.127 kb on + strand at 1957.127 kb on + strand at 1957.127 kb on + strand at 1957.127 kb on + strand at 1957.133 kb on - strand at 1957.135 kb on - strand at 1957.135 kb on - strand at 1957.135 kb on - strand at 1957.166 kb on + strand at 1957.186 kb on - strand at 1957.188 kb on - strand at 1957.188 kb on - strand at 1957.212 kb on + strand at 1957.234 kb on - strand at 1957.234 kb on - strand at 1957.234 kb on - strand at 1957.234 kb on - strand at 1957.234 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 1,955,397 + tRNA-Leu-2 SO_t035 0.61 -0.7 1,955,448 + -0.3 1,955,465 + -1.2 1,955,465 + -1.1 1,955,647 + +0.4 1,955,778 + -0.6 1,955,786 - +0.5 1,955,786 - -1.1 1,955,798 + -0.2 1,955,822 + +2.0 1,955,855 - -1.5 1,955,879 - -1.2 1,955,928 + +0.8 1,955,983 + +1.8 1,955,991 - -0.8 1,955,993 + SO1863 0.10 -3.0 1,956,008 - SO1863 0.16 -1.3 1,956,150 + SO1863 0.67 +0.3 1,956,174 + SO1863 0.76 +0.4 1,956,179 + SO1863 0.78 +0.8 1,956,181 - SO1863 0.78 -1.4 1,956,182 - SO1863 0.79 -1.3 1,956,185 + SO1863 0.80 -0.0 1,956,185 + SO1863 0.80 -0.5 1,956,185 + SO1863 0.80 -1.6 1,956,193 - SO1863 0.83 -0.5 1,956,193 - SO1863 0.83 -1.8 1,956,193 - SO1863 0.83 +0.3 1,956,195 + SO1863 0.83 -0.1 1,956,195 + SO1863 0.83 +0.0 1,956,225 + +0.5 1,956,228 - -1.1 1,956,233 - +1.0 1,956,233 - +2.0 1,956,233 - +2.0 1,956,272 + -1.1 1,956,277 + -0.9 1,956,298 - +1.0 1,956,316 - -0.3 1,956,327 + +1.4 1,956,374 - +0.2 1,956,379 + -0.6 1,956,384 + -0.0 1,956,397 - +1.3 1,956,400 + +1.5 1,956,400 + +1.6 1,956,400 + +1.1 1,956,400 + +0.8 1,956,400 + +0.7 1,956,400 + +0.8 1,956,407 - -0.2 1,956,407 - +0.1 1,956,407 - -0.3 1,956,408 - -2.3 1,956,408 - -0.0 1,956,408 - -0.8 1,956,408 - +2.0 1,956,408 - +0.7 1,956,408 - +0.1 1,956,408 - +0.3 1,956,408 - +0.9 1,956,408 - -0.3 1,956,408 - -0.1 1,956,408 - -0.3 1,956,408 - +0.3 1,956,408 - +1.8 1,956,445 + -1.4 1,956,445 + -2.0 1,956,498 - -2.3 1,956,531 + SO1864 0.14 -0.6 1,956,539 + SO1864 0.15 +0.8 1,956,615 + SO1864 0.26 +0.3 1,956,657 - SO1864 0.31 -1.2 1,956,659 + SO1864 0.32 -1.1 1,956,659 + SO1864 0.32 +1.7 1,956,659 + SO1864 0.32 +0.8 1,956,659 + SO1864 0.32 -1.6 1,956,659 + SO1864 0.32 +0.9 1,956,659 + SO1864 0.32 +0.6 1,956,667 - SO1864 0.33 +1.3 1,956,696 + SO1864 0.37 +1.2 1,956,696 + SO1864 0.37 -0.9 1,956,696 + SO1864 0.37 -2.1 1,956,696 + SO1864 0.37 -1.7 1,956,696 + SO1864 0.37 -0.4 1,956,696 + SO1864 0.37 -1.0 1,956,696 + SO1864 0.37 +0.2 1,956,696 + SO1864 0.37 +0.0 1,956,696 + SO1864 0.37 +1.5 1,956,697 + SO1864 0.37 -0.7 1,956,704 - SO1864 0.38 -1.2 1,956,704 - SO1864 0.38 -0.1 1,956,704 - SO1864 0.38 -2.1 1,956,704 - SO1864 0.38 +1.1 1,956,704 - SO1864 0.38 -0.8 1,956,704 - SO1864 0.38 +0.2 1,956,704 - SO1864 0.38 +0.0 1,956,705 - SO1864 0.38 +0.9 1,956,705 - SO1864 0.38 +0.7 1,956,705 - SO1864 0.38 +0.8 1,956,705 - SO1864 0.38 -2.3 1,956,761 - SO1864 0.46 -0.0 1,956,763 + SO1864 0.46 +2.4 1,956,763 + SO1864 0.46 -0.1 1,956,763 + SO1864 0.46 +0.7 1,956,771 - SO1864 0.47 +0.2 1,956,771 - SO1864 0.47 +0.9 1,956,771 - SO1864 0.47 -3.3 1,956,817 - SO1864 0.54 +0.1 1,956,841 - SO1864 0.57 +0.9 1,956,845 + SO1864 0.57 -1.4 1,956,870 - SO1864 0.61 -0.3 1,956,870 - SO1864 0.61 -1.3 1,956,878 - SO1864 0.62 -2.9 1,956,883 - SO1864 0.63 -0.6 1,956,900 - SO1864 0.65 -0.1 1,956,921 + SO1864 0.68 +0.7 1,956,945 - SO1864 0.71 +0.1 1,956,945 - SO1864 0.71 +0.4 1,956,953 + SO1864 0.72 +0.5 1,956,958 + SO1864 0.73 +0.3 1,956,958 + SO1864 0.73 -2.0 1,956,958 + SO1864 0.73 -1.8 1,956,958 + SO1864 0.73 +0.4 1,956,965 + SO1864 0.74 +0.2 1,956,966 - SO1864 0.74 -0.4 1,956,966 - SO1864 0.74 -0.5 1,956,966 - SO1864 0.74 -0.1 1,956,970 + SO1864 0.75 +1.2 1,956,970 + SO1864 0.75 -2.3 1,956,970 + SO1864 0.75 -0.9 1,956,970 + SO1864 0.75 -0.2 1,956,971 - SO1864 0.75 +1.5 1,956,973 - SO1864 0.75 -0.6 1,956,973 - SO1864 0.75 -0.6 1,956,973 - SO1864 0.75 -0.4 1,956,975 + SO1864 0.76 -0.3 1,956,978 - SO1864 0.76 -0.8 1,956,978 - SO1864 0.76 +1.2 1,956,978 - SO1864 0.76 -2.8 1,956,983 - SO1864 0.77 -0.8 1,956,988 + SO1864 0.77 +0.2 1,956,995 - SO1864 0.78 -0.5 1,957,006 + SO1864 0.80 -0.3 1,957,006 + SO1864 0.80 +0.5 1,957,006 + SO1864 0.80 -2.4 1,957,006 + SO1864 0.80 -0.0 1,957,006 + SO1864 0.80 -2.2 1,957,007 + SO1864 0.80 -0.7 1,957,014 + SO1864 0.81 -0.0 1,957,022 - SO1864 0.82 +0.8 1,957,023 - SO1864 0.82 +1.4 1,957,055 - SO1864 0.87 -0.6 1,957,107 + -2.1 1,957,107 - +1.5 1,957,115 + +0.6 1,957,127 + -2.7 1,957,127 + -0.2 1,957,127 + -1.0 1,957,127 + +0.7 1,957,127 + +1.1 1,957,133 - -0.3 1,957,135 - +0.3 1,957,135 - -1.0 1,957,135 - -1.3 1,957,166 + -0.0 1,957,186 - +0.8 1,957,188 - +1.0 1,957,188 - -1.7 1,957,212 + -0.2 1,957,234 - +1.4 1,957,234 - -0.9 1,957,234 - +2.6 1,957,234 - -1.4 1,957,234 - +1.2
Or see this region's nucleotide sequence