Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prkB and SO0879 are separated by 84 nucleotides SO0879 and SO0880 are separated by 9 nucleotides SO0880 and SO0881 are separated by 111 nucleotides SO0881 and SO0882 are separated by 46 nucleotides
SO0878: prkB - phosphoribulokinase (NCBI ptt file), at 907,976 to 908,863
prkB
SO0879: SO0879 - conserved hypothetical protein (NCBI ptt file), at 908,948 to 909,193
SO0879
SO0880: SO0880 - conserved hypothetical protein (NCBI ptt file), at 909,203 to 910,186
SO0880
SO0881: SO0881 - conserved hypothetical protein (NCBI ptt file), at 910,298 to 910,804
SO0881
SO0882: SO0882 - oxidoreductase, GMC family (NCBI ptt file), at 910,851 to 912,452
SO0882
Position (kb) 909
910
911 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 908.216 kb on - strand, within prkB at 908.218 kb on + strand, within prkB at 908.299 kb on + strand, within prkB at 908.350 kb on + strand, within prkB at 908.372 kb on - strand, within prkB at 908.431 kb on + strand, within prkB at 908.455 kb on - strand, within prkB at 908.465 kb on + strand, within prkB at 908.473 kb on - strand, within prkB at 908.473 kb on - strand, within prkB at 908.473 kb on - strand, within prkB at 908.557 kb on + strand, within prkB at 908.623 kb on + strand, within prkB at 908.631 kb on - strand, within prkB at 908.641 kb on - strand, within prkB at 908.760 kb on - strand, within prkB at 908.762 kb on + strand, within prkB at 908.822 kb on + strand at 908.830 kb on - strand at 908.872 kb on + strand at 909.142 kb on - strand, within SO0879 at 909.308 kb on + strand, within SO0880 at 909.412 kb on + strand, within SO0880 at 909.592 kb on + strand, within SO0880 at 909.703 kb on + strand, within SO0880 at 909.799 kb on - strand, within SO0880 at 909.826 kb on + strand, within SO0880 at 909.826 kb on + strand, within SO0880 at 909.834 kb on - strand, within SO0880 at 909.892 kb on + strand, within SO0880 at 909.900 kb on - strand, within SO0880 at 909.910 kb on - strand, within SO0880 at 909.947 kb on + strand, within SO0880 at 909.955 kb on + strand, within SO0880 at 909.969 kb on + strand, within SO0880 at 910.083 kb on - strand, within SO0880 at 910.083 kb on - strand, within SO0880 at 910.083 kb on - strand, within SO0880 at 910.109 kb on - strand at 910.166 kb on - strand at 910.305 kb on + strand at 910.377 kb on + strand, within SO0881 at 910.377 kb on + strand, within SO0881 at 910.402 kb on + strand, within SO0881 at 910.545 kb on - strand, within SO0881 at 910.654 kb on + strand, within SO0881 at 910.752 kb on + strand, within SO0881 at 910.760 kb on + strand at 910.760 kb on - strand at 910.760 kb on - strand at 910.774 kb on + strand at 910.774 kb on + strand at 910.782 kb on - strand at 910.895 kb on + strand at 910.896 kb on + strand at 910.898 kb on + strand at 911.083 kb on + strand, within SO0882
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 908,216 - prkB SO0878 0.27 -1.8 908,218 + prkB SO0878 0.27 -1.1 908,299 + prkB SO0878 0.36 -2.6 908,350 + prkB SO0878 0.42 -1.1 908,372 - prkB SO0878 0.45 -0.3 908,431 + prkB SO0878 0.51 -1.5 908,455 - prkB SO0878 0.54 -0.8 908,465 + prkB SO0878 0.55 -2.6 908,473 - prkB SO0878 0.56 -1.0 908,473 - prkB SO0878 0.56 +0.2 908,473 - prkB SO0878 0.56 -1.2 908,557 + prkB SO0878 0.65 -0.3 908,623 + prkB SO0878 0.73 -2.5 908,631 - prkB SO0878 0.74 -0.5 908,641 - prkB SO0878 0.75 -0.7 908,760 - prkB SO0878 0.88 -0.5 908,762 + prkB SO0878 0.89 -1.5 908,822 + -2.2 908,830 - -1.9 908,872 + +0.1 909,142 - SO0879 0.79 -1.7 909,308 + SO0880 0.11 +1.0 909,412 + SO0880 0.21 +0.8 909,592 + SO0880 0.40 -0.7 909,703 + SO0880 0.51 +0.8 909,799 - SO0880 0.61 -0.4 909,826 + SO0880 0.63 -2.1 909,826 + SO0880 0.63 +0.0 909,834 - SO0880 0.64 +0.0 909,892 + SO0880 0.70 +2.6 909,900 - SO0880 0.71 +0.2 909,910 - SO0880 0.72 -0.9 909,947 + SO0880 0.76 -3.0 909,955 + SO0880 0.76 +0.4 909,969 + SO0880 0.78 -0.3 910,083 - SO0880 0.89 -0.4 910,083 - SO0880 0.89 +0.6 910,083 - SO0880 0.89 +0.0 910,109 - -0.6 910,166 - +1.0 910,305 + +0.3 910,377 + SO0881 0.16 +0.3 910,377 + SO0881 0.16 +0.7 910,402 + SO0881 0.21 +0.5 910,545 - SO0881 0.49 -0.1 910,654 + SO0881 0.70 +1.1 910,752 + SO0881 0.90 -0.3 910,760 + +0.5 910,760 - +1.1 910,760 - -1.0 910,774 + +0.6 910,774 + +0.1 910,782 - +1.4 910,895 + +0.1 910,896 + -1.4 910,898 + -1.5 911,083 + SO0882 0.14 +0.5
Or see this region's nucleotide sequence