Strain Fitness in Shewanella oneidensis MR-1 around SO0011

Experiment: L-Methionine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecF and gyrB are separated by 16 nucleotidesgyrB and SO0012 are separated by 73 nucleotides SO0010: recF - DNA replication and repair protein RecF (NCBI ptt file), at 9,539 to 10,621 recF SO0011: gyrB - DNA gyrase, B subunit (NCBI ptt file), at 10,638 to 13,055 gyrB SO0012: SO0012 - glutathione S-transferase family protein (NCBI ptt file), at 13,129 to 13,791 SO0012 Position (kb) 10 11 12 13 14Strain fitness (log2 ratio) -3 -2 -1 0 1at 9.746 kb on - strand, within recFat 9.756 kb on + strand, within recFat 9.764 kb on - strand, within recFat 9.842 kb on + strand, within recFat 9.863 kb on + strand, within recFat 9.966 kb on - strand, within recFat 10.144 kb on + strand, within recFat 10.144 kb on + strand, within recFat 10.144 kb on + strand, within recFat 10.177 kb on + strand, within recFat 10.259 kb on + strand, within recFat 10.291 kb on + strand, within recFat 13.147 kb on - strandat 13.232 kb on - strand, within SO0012at 13.289 kb on + strand, within SO0012at 13.377 kb on + strand, within SO0012at 13.427 kb on - strand, within SO0012at 13.542 kb on + strand, within SO0012at 13.550 kb on - strand, within SO0012at 13.635 kb on - strand, within SO0012at 13.778 kb on + strandat 13.864 kb on - strandat 13.955 kb on + strandat 13.977 kb on - strandat 14.030 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Methionine (N)
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9,746 - recF SO0010 0.19 +1.1
9,756 + recF SO0010 0.20 -2.2
9,764 - recF SO0010 0.21 +0.1
9,842 + recF SO0010 0.28 +0.4
9,863 + recF SO0010 0.30 +0.5
9,966 - recF SO0010 0.39 -1.1
10,144 + recF SO0010 0.56 -0.8
10,144 + recF SO0010 0.56 -2.5
10,144 + recF SO0010 0.56 +1.1
10,177 + recF SO0010 0.59 -3.2
10,259 + recF SO0010 0.66 -2.1
10,291 + recF SO0010 0.69 -2.0
13,147 - +0.3
13,232 - SO0012 0.16 -0.4
13,289 + SO0012 0.24 -3.6
13,377 + SO0012 0.37 -0.3
13,427 - SO0012 0.45 +0.1
13,542 + SO0012 0.62 -0.6
13,550 - SO0012 0.63 -2.1
13,635 - SO0012 0.76 +0.9
13,778 + -0.6
13,864 - +0.6
13,955 + -0.8
13,977 - +0.5
14,030 - +1.2

Or see this region's nucleotide sequence