Experiment: Minimal media, pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and Sama_1609 are separated by 48 nucleotides Sama_1609 and Sama_R0061 are separated by 228 nucleotides Sama_R0061 and Sama_R0062 are separated by 30 nucleotides Sama_R0062 and Sama_R0063 are separated by 38 nucleotides Sama_R0063 and Sama_1610 are separated by 245 nucleotides
Sama_1608: uvrC - excinuclease ABC subunit C (RefSeq), at 1,965,437 to 1,967,257
uvrC
Sama_1609: Sama_1609 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (RefSeq), at 1,967,306 to 1,967,854
_1609
Sama_R0061: Sama_R0061 - tRNA-Gly (RefSeq), at 1,968,083 to 1,968,158
_R0061
Sama_R0062: Sama_R0062 - tRNA-Cys (RefSeq), at 1,968,189 to 1,968,262
_R0062
Sama_R0063: Sama_R0063 - tRNA-Leu (RefSeq), at 1,968,301 to 1,968,387
_R0063
Sama_1610: Sama_1610 - hypothetical protein (RefSeq), at 1,968,633 to 1,969,796
_1610
Position (kb) 1967
1968 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1966.311 kb on + strand, within uvrC at 1966.311 kb on + strand, within uvrC at 1966.311 kb on + strand, within uvrC at 1966.334 kb on + strand, within uvrC at 1966.378 kb on - strand, within uvrC at 1966.488 kb on + strand, within uvrC at 1966.501 kb on + strand, within uvrC at 1966.540 kb on + strand, within uvrC at 1966.562 kb on - strand, within uvrC at 1966.603 kb on - strand, within uvrC at 1966.603 kb on - strand, within uvrC at 1966.625 kb on - strand, within uvrC at 1966.700 kb on + strand, within uvrC at 1966.714 kb on + strand, within uvrC at 1966.714 kb on + strand, within uvrC at 1966.714 kb on + strand, within uvrC at 1966.714 kb on + strand, within uvrC at 1966.715 kb on - strand, within uvrC at 1966.715 kb on - strand, within uvrC at 1966.717 kb on - strand, within uvrC at 1966.726 kb on + strand, within uvrC at 1966.727 kb on - strand, within uvrC at 1966.727 kb on - strand, within uvrC at 1966.742 kb on + strand, within uvrC at 1966.781 kb on - strand, within uvrC at 1966.861 kb on - strand, within uvrC at 1967.020 kb on + strand, within uvrC at 1967.086 kb on + strand at 1967.087 kb on - strand at 1967.147 kb on + strand at 1967.880 kb on + strand at 1967.883 kb on + strand at 1967.883 kb on + strand at 1967.883 kb on + strand at 1967.883 kb on + strand at 1967.894 kb on + strand at 1968.021 kb on + strand at 1968.021 kb on + strand at 1968.021 kb on + strand at 1968.034 kb on + strand at 1968.068 kb on + strand at 1968.125 kb on + strand, within Sama_R0061 at 1968.394 kb on + strand at 1968.412 kb on - strand at 1968.500 kb on - strand at 1968.548 kb on + strand at 1968.548 kb on + strand at 1968.548 kb on + strand at 1968.548 kb on + strand at 1968.548 kb on + strand at 1968.548 kb on + strand at 1968.548 kb on + strand at 1968.548 kb on + strand at 1968.548 kb on + strand at 1968.549 kb on - strand at 1968.549 kb on - strand at 1968.551 kb on - strand at 1968.594 kb on + strand at 1968.644 kb on + strand at 1968.645 kb on - strand at 1968.739 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media, pH7 remove 1,966,311 + uvrC Sama_1608 0.48 -1.2 1,966,311 + uvrC Sama_1608 0.48 -0.5 1,966,311 + uvrC Sama_1608 0.48 -1.9 1,966,334 + uvrC Sama_1608 0.49 +0.5 1,966,378 - uvrC Sama_1608 0.52 -2.3 1,966,488 + uvrC Sama_1608 0.58 +0.9 1,966,501 + uvrC Sama_1608 0.58 -2.3 1,966,540 + uvrC Sama_1608 0.61 -0.1 1,966,562 - uvrC Sama_1608 0.62 -2.1 1,966,603 - uvrC Sama_1608 0.64 -0.1 1,966,603 - uvrC Sama_1608 0.64 -1.5 1,966,625 - uvrC Sama_1608 0.65 -0.9 1,966,700 + uvrC Sama_1608 0.69 -1.1 1,966,714 + uvrC Sama_1608 0.70 +0.1 1,966,714 + uvrC Sama_1608 0.70 +2.0 1,966,714 + uvrC Sama_1608 0.70 +0.3 1,966,714 + uvrC Sama_1608 0.70 +0.5 1,966,715 - uvrC Sama_1608 0.70 -1.5 1,966,715 - uvrC Sama_1608 0.70 -0.6 1,966,717 - uvrC Sama_1608 0.70 -1.7 1,966,726 + uvrC Sama_1608 0.71 -0.7 1,966,727 - uvrC Sama_1608 0.71 +0.5 1,966,727 - uvrC Sama_1608 0.71 +0.9 1,966,742 + uvrC Sama_1608 0.72 +1.7 1,966,781 - uvrC Sama_1608 0.74 -0.9 1,966,861 - uvrC Sama_1608 0.78 -0.8 1,967,020 + uvrC Sama_1608 0.87 +0.0 1,967,086 + +0.5 1,967,087 - -0.9 1,967,147 + +1.0 1,967,880 + -0.1 1,967,883 + +0.2 1,967,883 + +0.7 1,967,883 + -1.1 1,967,883 + +1.3 1,967,894 + -0.2 1,968,021 + +1.5 1,968,021 + +1.4 1,968,021 + -0.0 1,968,034 + -1.4 1,968,068 + +0.7 1,968,125 + Sama_R0061 0.55 -3.5 1,968,394 + -0.0 1,968,412 - -1.1 1,968,500 - -1.3 1,968,548 + -0.1 1,968,548 + +1.0 1,968,548 + -0.9 1,968,548 + -1.6 1,968,548 + +1.2 1,968,548 + -0.9 1,968,548 + -0.3 1,968,548 + -0.6 1,968,548 + -2.7 1,968,549 - +1.1 1,968,549 - -0.6 1,968,551 - -0.4 1,968,594 + -0.6 1,968,644 + +0.2 1,968,645 - +0.7 1,968,739 - +1.2
Or see this region's nucleotide sequence