Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3159 and rfbC are separated by 19 nucleotides rfbC and SO3161 overlap by 14 nucleotides SO3161 and SO3162 are separated by 192 nucleotides SO3162 and SO3163 are separated by 210 nucleotides SO3163 and SO3164 are separated by 293 nucleotides
SO3159: SO3159 - conserved hypothetical protein (NCBI ptt file), at 3,295,149 to 3,297,527
SO3159
SO3160: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase (NCBI ptt file), at 3,297,547 to 3,298,086
rfbC
SO3161: SO3161 - glucose-1-phosphate thymidylyltransferase-related protein (NCBI ptt file), at 3,298,073 to 3,298,267
SO3161
SO3162: SO3162 - sensor histidine kinase (NCBI ptt file), at 3,298,460 to 3,299,821
SO3162
SO3163: SO3163 - lipoprotein (NCBI ptt file), at 3,300,032 to 3,300,484
SO3163
SO3164: SO3164 - conserved hypothetical protein (NCBI ptt file), at 3,300,778 to 3,301,545
SO3164
Position (kb)
3298
3299
3300 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3298.109 kb on + strand, within SO3161 at 3298.146 kb on + strand, within SO3161 at 3298.178 kb on + strand, within SO3161 at 3298.444 kb on - strand at 3298.501 kb on - strand at 3298.505 kb on - strand at 3298.567 kb on + strand at 3298.568 kb on + strand at 3298.568 kb on + strand at 3298.568 kb on + strand at 3298.568 kb on + strand at 3298.568 kb on + strand at 3298.568 kb on + strand at 3298.568 kb on + strand at 3298.568 kb on + strand at 3298.576 kb on - strand at 3298.576 kb on - strand at 3298.576 kb on - strand at 3298.576 kb on - strand at 3298.576 kb on - strand at 3298.576 kb on - strand at 3298.576 kb on - strand at 3298.797 kb on + strand, within SO3162 at 3298.822 kb on + strand, within SO3162 at 3298.930 kb on - strand, within SO3162 at 3298.958 kb on + strand, within SO3162 at 3298.966 kb on - strand, within SO3162 at 3299.002 kb on + strand, within SO3162 at 3299.002 kb on + strand, within SO3162 at 3299.010 kb on - strand, within SO3162 at 3299.033 kb on + strand, within SO3162 at 3299.076 kb on + strand, within SO3162 at 3299.129 kb on + strand, within SO3162 at 3299.135 kb on - strand, within SO3162 at 3299.137 kb on - strand, within SO3162 at 3299.137 kb on - strand, within SO3162 at 3299.137 kb on - strand, within SO3162 at 3299.139 kb on + strand, within SO3162 at 3299.139 kb on + strand, within SO3162 at 3299.147 kb on - strand, within SO3162 at 3299.154 kb on - strand, within SO3162 at 3299.164 kb on + strand, within SO3162 at 3299.357 kb on + strand, within SO3162 at 3299.370 kb on - strand, within SO3162 at 3299.399 kb on + strand, within SO3162 at 3299.473 kb on - strand, within SO3162 at 3299.479 kb on - strand, within SO3162 at 3299.483 kb on + strand, within SO3162 at 3299.521 kb on - strand, within SO3162 at 3299.540 kb on - strand, within SO3162 at 3299.540 kb on - strand, within SO3162 at 3299.540 kb on - strand, within SO3162 at 3299.617 kb on - strand, within SO3162 at 3299.644 kb on + strand, within SO3162 at 3299.644 kb on + strand, within SO3162 at 3299.652 kb on - strand, within SO3162 at 3299.652 kb on - strand, within SO3162 at 3299.657 kb on - strand, within SO3162 at 3299.737 kb on + strand at 3299.818 kb on + strand at 3299.843 kb on + strand at 3299.851 kb on - strand at 3299.851 kb on - strand at 3299.855 kb on + strand at 3299.889 kb on + strand at 3299.922 kb on - strand at 3299.922 kb on - strand at 3299.931 kb on + strand at 3299.939 kb on - strand at 3300.025 kb on - strand at 3300.074 kb on + strand at 3300.207 kb on - strand, within SO3163 at 3300.207 kb on - strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.217 kb on - strand, within SO3163 at 3300.217 kb on - strand, within SO3163 at 3300.253 kb on + strand, within SO3163 at 3300.253 kb on + strand, within SO3163 at 3300.260 kb on - strand, within SO3163 at 3300.462 kb on - strand at 3300.512 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 3,298,109 + SO3161 0.18 +2.0 3,298,146 + SO3161 0.37 +1.5 3,298,178 + SO3161 0.54 +1.0 3,298,444 - +1.6 3,298,501 - -1.2 3,298,505 - +0.6 3,298,567 + -0.1 3,298,568 + -1.1 3,298,568 + +0.9 3,298,568 + -1.4 3,298,568 + +0.3 3,298,568 + -1.4 3,298,568 + +1.1 3,298,568 + -0.9 3,298,568 + -0.9 3,298,576 - -0.1 3,298,576 - -0.8 3,298,576 - -0.1 3,298,576 - +0.1 3,298,576 - -0.2 3,298,576 - +0.4 3,298,576 - -0.1 3,298,797 + SO3162 0.25 -1.7 3,298,822 + SO3162 0.27 +0.8 3,298,930 - SO3162 0.35 +0.2 3,298,958 + SO3162 0.37 +0.3 3,298,966 - SO3162 0.37 -0.1 3,299,002 + SO3162 0.40 -1.1 3,299,002 + SO3162 0.40 -0.5 3,299,010 - SO3162 0.40 +0.9 3,299,033 + SO3162 0.42 +0.6 3,299,076 + SO3162 0.45 +0.2 3,299,129 + SO3162 0.49 +0.6 3,299,135 - SO3162 0.50 +1.1 3,299,137 - SO3162 0.50 -1.3 3,299,137 - SO3162 0.50 +0.5 3,299,137 - SO3162 0.50 +0.5 3,299,139 + SO3162 0.50 +0.9 3,299,139 + SO3162 0.50 +1.8 3,299,147 - SO3162 0.50 +0.2 3,299,154 - SO3162 0.51 +0.2 3,299,164 + SO3162 0.52 -0.4 3,299,357 + SO3162 0.66 -0.1 3,299,370 - SO3162 0.67 +0.9 3,299,399 + SO3162 0.69 -0.3 3,299,473 - SO3162 0.74 +0.3 3,299,479 - SO3162 0.75 -0.9 3,299,483 + SO3162 0.75 -0.1 3,299,521 - SO3162 0.78 -0.6 3,299,540 - SO3162 0.79 +1.0 3,299,540 - SO3162 0.79 +0.7 3,299,540 - SO3162 0.79 +1.6 3,299,617 - SO3162 0.85 +4.1 3,299,644 + SO3162 0.87 +0.2 3,299,644 + SO3162 0.87 +0.5 3,299,652 - SO3162 0.88 +0.9 3,299,652 - SO3162 0.88 +0.7 3,299,657 - SO3162 0.88 -0.5 3,299,737 + +1.9 3,299,818 + +0.4 3,299,843 + +0.4 3,299,851 - -1.9 3,299,851 - +1.6 3,299,855 + +0.4 3,299,889 + +0.3 3,299,922 - -0.7 3,299,922 - +0.3 3,299,931 + -0.9 3,299,939 - -0.6 3,300,025 - -0.5 3,300,074 + -0.3 3,300,207 - SO3163 0.39 -0.4 3,300,207 - SO3163 0.39 +0.4 3,300,209 + SO3163 0.39 -0.7 3,300,209 + SO3163 0.39 -0.7 3,300,209 + SO3163 0.39 +2.1 3,300,209 + SO3163 0.39 +0.5 3,300,209 + SO3163 0.39 +1.3 3,300,209 + SO3163 0.39 +0.6 3,300,209 + SO3163 0.39 -0.5 3,300,217 - SO3163 0.41 +1.4 3,300,217 - SO3163 0.41 -0.0 3,300,253 + SO3163 0.49 +0.2 3,300,253 + SO3163 0.49 +1.2 3,300,260 - SO3163 0.50 -0.3 3,300,462 - +1.2 3,300,512 + +2.4
Or see this region's nucleotide sequence