Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2308 and crcB overlap by 8 nucleotides crcB and serS are separated by 18 nucleotides serS and SO2311 are separated by 18 nucleotides SO2311 and SO2312 are separated by 78 nucleotides
SO2308: SO2308 - ATPase, AAA family (NCBI ptt file), at 2,420,521 to 2,421,858
SO2308
SO2309: crcB - crcB protein (NCBI ptt file), at 2,421,851 to 2,422,225
crcB
SO2310: serS - seryl-tRNA synthetase (NCBI ptt file), at 2,422,244 to 2,423,530
serS
SO2311: SO2311 - hypothetical protein (NCBI ptt file), at 2,423,549 to 2,423,671
SO2311
SO2312: SO2312 - serine protease inhibitor, ecotin (NCBI ptt file), at 2,423,750 to 2,424,301
SO2312
Position (kb)
2422
2423
2424 Strain fitness (log2 ratio)
-2
-1
0
1 at 2421.549 kb on + strand, within SO2308 at 2421.612 kb on - strand, within SO2308 at 2421.689 kb on + strand, within SO2308 at 2421.699 kb on + strand, within SO2308 at 2421.826 kb on + strand at 2421.867 kb on - strand at 2421.874 kb on - strand at 2421.877 kb on + strand at 2421.995 kb on + strand, within crcB at 2423.542 kb on - strand at 2423.542 kb on - strand at 2423.547 kb on - strand at 2423.559 kb on - strand at 2423.579 kb on + strand, within SO2311 at 2423.632 kb on + strand, within SO2311 at 2423.635 kb on - strand, within SO2311 at 2423.640 kb on - strand, within SO2311 at 2423.640 kb on - strand, within SO2311 at 2423.649 kb on - strand, within SO2311 at 2423.786 kb on - strand at 2423.803 kb on - strand at 2423.808 kb on - strand, within SO2312 at 2423.854 kb on + strand, within SO2312 at 2423.854 kb on + strand, within SO2312 at 2423.907 kb on - strand, within SO2312 at 2423.907 kb on - strand, within SO2312 at 2423.969 kb on + strand, within SO2312 at 2423.972 kb on - strand, within SO2312 at 2423.977 kb on - strand, within SO2312 at 2424.032 kb on + strand, within SO2312 at 2424.039 kb on - strand, within SO2312 at 2424.040 kb on - strand, within SO2312 at 2424.062 kb on - strand, within SO2312 at 2424.086 kb on - strand, within SO2312 at 2424.103 kb on + strand, within SO2312 at 2424.219 kb on - strand, within SO2312 at 2424.250 kb on + strand at 2424.253 kb on - strand at 2424.257 kb on + strand at 2424.265 kb on - strand at 2424.387 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,421,549 + SO2308 0.77 -0.5 2,421,612 - SO2308 0.82 +0.0 2,421,689 + SO2308 0.87 -0.6 2,421,699 + SO2308 0.88 -0.4 2,421,826 + +1.1 2,421,867 - -1.2 2,421,874 - +0.1 2,421,877 + -2.4 2,421,995 + crcB SO2309 0.38 +0.5 2,423,542 - -2.5 2,423,542 - +1.3 2,423,547 - -0.8 2,423,559 - +0.6 2,423,579 + SO2311 0.24 +0.2 2,423,632 + SO2311 0.67 +0.4 2,423,635 - SO2311 0.70 +1.2 2,423,640 - SO2311 0.74 -0.2 2,423,640 - SO2311 0.74 +0.0 2,423,649 - SO2311 0.81 +0.1 2,423,786 - -0.2 2,423,803 - +1.5 2,423,808 - SO2312 0.11 -1.5 2,423,854 + SO2312 0.19 +1.1 2,423,854 + SO2312 0.19 +0.3 2,423,907 - SO2312 0.28 -0.9 2,423,907 - SO2312 0.28 +0.5 2,423,969 + SO2312 0.40 +0.1 2,423,972 - SO2312 0.40 -1.2 2,423,977 - SO2312 0.41 +0.1 2,424,032 + SO2312 0.51 -1.0 2,424,039 - SO2312 0.52 -0.1 2,424,040 - SO2312 0.53 -1.7 2,424,062 - SO2312 0.57 +0.5 2,424,086 - SO2312 0.61 -0.2 2,424,103 + SO2312 0.64 +1.5 2,424,219 - SO2312 0.85 +0.1 2,424,250 + +0.6 2,424,253 - +0.8 2,424,257 + -0.1 2,424,265 - -0.2 2,424,387 - +0.6
Or see this region's nucleotide sequence