Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2109 and SO2110 are separated by 122 nucleotides SO2110 and SO2111 overlap by 50 nucleotides SO2111 and rplY are separated by 152 nucleotides rplY and SO2113 are separated by 397 nucleotides
SO2109: SO2109 - conserved hypothetical protein (NCBI ptt file), at 2,208,142 to 2,209,650
SO2109
SO2110: SO2110 - conserved hypothetical protein (NCBI ptt file), at 2,209,773 to 2,210,321
SO2110
SO2111: SO2111 - hypothetical protein (NCBI ptt file), at 2,210,272 to 2,210,706
SO2111
SO2112: rplY - ribosomal protein L25 (NCBI ptt file), at 2,210,859 to 2,211,305
rplY
SO2113: SO2113 - conserved hypothetical protein (NCBI ptt file), at 2,211,703 to 2,215,860
SO2113
Position (kb)
2210
2211 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2209.282 kb on + strand, within SO2109 at 2209.507 kb on - strand at 2209.554 kb on - strand at 2209.601 kb on + strand at 2209.609 kb on - strand at 2209.703 kb on + strand at 2209.789 kb on - strand at 2209.822 kb on - strand at 2209.826 kb on + strand at 2209.835 kb on - strand, within SO2110 at 2209.948 kb on + strand, within SO2110 at 2209.956 kb on - strand, within SO2110 at 2210.024 kb on + strand, within SO2110 at 2210.242 kb on + strand, within SO2110 at 2210.361 kb on + strand, within SO2111 at 2210.471 kb on - strand, within SO2111 at 2210.490 kb on + strand, within SO2111 at 2210.905 kb on - strand, within rplY at 2211.610 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,209,282 + SO2109 0.76 +1.2 2,209,507 - -0.4 2,209,554 - +0.1 2,209,601 + -0.6 2,209,609 - +0.3 2,209,703 + +0.6 2,209,789 - +1.9 2,209,822 - +0.6 2,209,826 + -1.0 2,209,835 - SO2110 0.11 +1.1 2,209,948 + SO2110 0.32 +1.1 2,209,956 - SO2110 0.33 +0.8 2,210,024 + SO2110 0.46 +0.2 2,210,242 + SO2110 0.85 +1.6 2,210,361 + SO2111 0.20 -0.1 2,210,471 - SO2111 0.46 -0.3 2,210,490 + SO2111 0.50 -2.1 2,210,905 - rplY SO2112 0.10 +1.3 2,211,610 + -0.4
Or see this region's nucleotide sequence