Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnS and SO1787 are separated by 143 nucleotides SO1787 and miaE are separated by 278 nucleotides
SO1786: glnS - glutaminyl-tRNA synthetase (NCBI ptt file), at 1,872,353 to 1,874,023
glnS
SO1787: SO1787 - conserved hypothetical protein (NCBI ptt file), at 1,874,167 to 1,874,763
SO1787
SO1788: miaE - tRNA-(MS[2]IO[6]A)-hydroxylase (NCBI ptt file), at 1,875,042 to 1,875,827
miaE
Position (kb)
1874
1875 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1874.021 kb on - strand at 1874.216 kb on - strand at 1874.217 kb on + strand at 1874.217 kb on + strand at 1874.236 kb on - strand, within SO1787 at 1874.244 kb on - strand, within SO1787 at 1874.244 kb on - strand, within SO1787 at 1874.318 kb on - strand, within SO1787 at 1874.323 kb on - strand, within SO1787 at 1874.333 kb on - strand, within SO1787 at 1874.333 kb on - strand, within SO1787 at 1874.333 kb on - strand, within SO1787 at 1874.389 kb on - strand, within SO1787 at 1874.391 kb on + strand, within SO1787 at 1874.399 kb on - strand, within SO1787 at 1874.513 kb on - strand, within SO1787 at 1874.616 kb on - strand, within SO1787 at 1874.700 kb on + strand, within SO1787 at 1874.700 kb on + strand, within SO1787 at 1874.708 kb on - strand at 1874.740 kb on - strand at 1874.740 kb on - strand at 1874.891 kb on - strand at 1874.976 kb on + strand at 1874.983 kb on - strand at 1875.071 kb on - strand at 1875.426 kb on - strand, within miaE at 1875.567 kb on - strand, within miaE at 1875.734 kb on - strand, within miaE
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 1,874,021 - +0.1 1,874,216 - -0.8 1,874,217 + +0.8 1,874,217 + +0.2 1,874,236 - SO1787 0.12 -0.1 1,874,244 - SO1787 0.13 +0.4 1,874,244 - SO1787 0.13 +0.2 1,874,318 - SO1787 0.25 +0.4 1,874,323 - SO1787 0.26 +0.1 1,874,333 - SO1787 0.28 +0.5 1,874,333 - SO1787 0.28 +0.1 1,874,333 - SO1787 0.28 +0.9 1,874,389 - SO1787 0.37 +1.3 1,874,391 + SO1787 0.38 +1.3 1,874,399 - SO1787 0.39 -2.4 1,874,513 - SO1787 0.58 +4.6 1,874,616 - SO1787 0.75 +0.5 1,874,700 + SO1787 0.89 +1.3 1,874,700 + SO1787 0.89 -0.8 1,874,708 - +0.7 1,874,740 - -0.1 1,874,740 - -0.4 1,874,891 - -0.1 1,874,976 + +0.2 1,874,983 - -0.9 1,875,071 - +0.6 1,875,426 - miaE SO1788 0.49 +0.1 1,875,567 - miaE SO1788 0.67 -0.2 1,875,734 - miaE SO1788 0.88 +0.1
Or see this region's nucleotide sequence