Strain Fitness in Shewanella oneidensis MR-1 around SO1353

Experiment: L-Leucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpdxJ and acpS overlap by 1 nucleotidesacpS and SO1353 are separated by 170 nucleotidesSO1353 and SO1354 are separated by 67 nucleotides SO1351: pdxJ - pyridoxal phosphate biosynthetic protein PdxJ (NCBI ptt file), at 1,407,463 to 1,408,200 pdxJ SO1352: acpS - holo-(acyl-carrier protein) synthase (NCBI ptt file), at 1,408,200 to 1,408,583 acpS SO1353: SO1353 - conserved hypothetical protein (NCBI ptt file), at 1,408,754 to 1,409,281 SO1353 SO1354: SO1354 - hemolysin protein, putative (NCBI ptt file), at 1,409,349 to 1,410,620 SO1354 Position (kb) 1408 1409 1410Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1408.297 kb on - strand, within acpSat 1408.490 kb on - strand, within acpSat 1408.599 kb on + strandat 1408.736 kb on + strandat 1408.922 kb on + strand, within SO1353at 1409.008 kb on + strand, within SO1353at 1409.010 kb on + strand, within SO1353at 1409.021 kb on - strand, within SO1353at 1409.021 kb on - strand, within SO1353at 1409.121 kb on - strand, within SO1353at 1409.217 kb on - strand, within SO1353at 1409.362 kb on + strandat 1409.479 kb on - strand, within SO1354at 1409.511 kb on - strand, within SO1354at 1409.583 kb on + strand, within SO1354at 1409.729 kb on - strand, within SO1354at 1409.773 kb on - strand, within SO1354at 1409.847 kb on + strand, within SO1354at 1409.899 kb on + strand, within SO1354at 1410.112 kb on - strand, within SO1354at 1410.112 kb on - strand, within SO1354at 1410.112 kb on - strand, within SO1354

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (N)
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1,408,297 - acpS SO1352 0.25 -1.2
1,408,490 - acpS SO1352 0.76 +0.4
1,408,599 + +0.0
1,408,736 + -0.3
1,408,922 + SO1353 0.32 -0.6
1,409,008 + SO1353 0.48 -2.0
1,409,010 + SO1353 0.48 +1.2
1,409,021 - SO1353 0.51 +1.6
1,409,021 - SO1353 0.51 +0.9
1,409,121 - SO1353 0.70 +0.7
1,409,217 - SO1353 0.88 -0.7
1,409,362 + +1.1
1,409,479 - SO1354 0.10 -0.5
1,409,511 - SO1354 0.13 -0.2
1,409,583 + SO1354 0.18 +0.1
1,409,729 - SO1354 0.30 +1.4
1,409,773 - SO1354 0.33 -2.1
1,409,847 + SO1354 0.39 +0.2
1,409,899 + SO1354 0.43 -0.7
1,410,112 - SO1354 0.60 -0.0
1,410,112 - SO1354 0.60 +4.1
1,410,112 - SO1354 0.60 +0.5

Or see this region's nucleotide sequence