Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0438 and SO0439 overlap by 4 nucleotides SO0439 and SO0440 are separated by 97 nucleotides SO0440 and purD are separated by 178 nucleotides
SO0438: SO0438 - oxidoreductase, short chain dehydrogenase/reductase family (NCBI ptt file), at 463,857 to 464,660
SO0438
SO0439: SO0439 - hypothetical protein (NCBI ptt file), at 464,657 to 464,932
SO0439
SO0440: SO0440 - conserved hypothetical protein (NCBI ptt file), at 465,030 to 468,251
SO0440
SO0441: purD - phosphoribosylamine--glycine ligase (NCBI ptt file), at 468,430 to 469,728
purD
Position (kb) 465
466
467
468
469 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 464.053 kb on + strand, within SO0438 at 464.149 kb on + strand, within SO0438 at 464.258 kb on + strand, within SO0438 at 464.260 kb on + strand, within SO0438 at 464.291 kb on + strand, within SO0438 at 464.299 kb on - strand, within SO0438 at 464.299 kb on - strand, within SO0438 at 464.299 kb on - strand, within SO0438 at 464.299 kb on - strand, within SO0438 at 464.456 kb on + strand, within SO0438 at 464.481 kb on + strand, within SO0438 at 464.511 kb on - strand, within SO0438 at 464.593 kb on + strand at 464.601 kb on - strand at 464.601 kb on - strand at 464.646 kb on + strand at 464.914 kb on + strand at 464.916 kb on + strand at 464.946 kb on + strand at 465.059 kb on - strand at 465.082 kb on - strand at 465.107 kb on + strand at 465.107 kb on + strand at 465.108 kb on + strand at 465.108 kb on + strand at 465.116 kb on - strand at 465.116 kb on - strand at 465.175 kb on + strand at 465.228 kb on - strand at 465.273 kb on - strand at 465.316 kb on - strand at 465.507 kb on + strand, within SO0440 at 465.549 kb on + strand, within SO0440 at 465.649 kb on - strand, within SO0440 at 465.758 kb on - strand, within SO0440 at 465.777 kb on + strand, within SO0440 at 465.813 kb on + strand, within SO0440 at 465.813 kb on + strand, within SO0440 at 465.813 kb on + strand, within SO0440 at 465.890 kb on - strand, within SO0440 at 465.948 kb on - strand, within SO0440 at 465.948 kb on - strand, within SO0440 at 466.052 kb on + strand, within SO0440 at 466.128 kb on + strand, within SO0440 at 466.128 kb on + strand, within SO0440 at 466.128 kb on + strand, within SO0440 at 466.128 kb on + strand, within SO0440 at 466.136 kb on - strand, within SO0440 at 466.136 kb on - strand, within SO0440 at 466.136 kb on - strand, within SO0440 at 466.136 kb on - strand, within SO0440 at 466.136 kb on - strand, within SO0440 at 466.136 kb on - strand, within SO0440 at 466.136 kb on - strand, within SO0440 at 466.138 kb on + strand, within SO0440 at 466.162 kb on + strand, within SO0440 at 466.162 kb on + strand, within SO0440 at 466.170 kb on - strand, within SO0440 at 466.264 kb on - strand, within SO0440 at 466.419 kb on + strand, within SO0440 at 466.420 kb on + strand, within SO0440 at 466.427 kb on - strand, within SO0440 at 466.427 kb on - strand, within SO0440 at 466.427 kb on - strand, within SO0440 at 466.475 kb on + strand, within SO0440 at 466.475 kb on + strand, within SO0440 at 466.525 kb on + strand, within SO0440 at 466.650 kb on - strand, within SO0440 at 466.650 kb on - strand, within SO0440 at 466.678 kb on - strand, within SO0440 at 466.689 kb on + strand, within SO0440 at 466.763 kb on + strand, within SO0440 at 466.763 kb on + strand, within SO0440 at 466.771 kb on - strand, within SO0440 at 466.771 kb on - strand, within SO0440 at 466.771 kb on - strand, within SO0440 at 466.888 kb on + strand, within SO0440 at 466.930 kb on - strand, within SO0440 at 466.945 kb on - strand, within SO0440 at 466.986 kb on - strand, within SO0440 at 466.994 kb on - strand, within SO0440 at 467.045 kb on - strand, within SO0440 at 467.152 kb on + strand, within SO0440 at 467.236 kb on + strand, within SO0440 at 467.263 kb on + strand, within SO0440 at 467.271 kb on - strand, within SO0440 at 467.271 kb on - strand, within SO0440 at 467.324 kb on - strand, within SO0440 at 467.508 kb on + strand, within SO0440 at 467.527 kb on + strand, within SO0440 at 467.533 kb on - strand, within SO0440 at 467.545 kb on - strand, within SO0440 at 467.575 kb on + strand, within SO0440 at 467.625 kb on + strand, within SO0440 at 467.651 kb on + strand, within SO0440 at 467.669 kb on + strand, within SO0440 at 467.669 kb on - strand, within SO0440 at 467.788 kb on + strand, within SO0440 at 467.796 kb on - strand, within SO0440 at 467.845 kb on - strand, within SO0440 at 467.879 kb on + strand, within SO0440 at 467.930 kb on + strand at 468.060 kb on - strand at 468.157 kb on + strand at 468.167 kb on - strand at 468.175 kb on - strand at 468.204 kb on - strand at 468.219 kb on + strand at 468.421 kb on + strand at 468.429 kb on - strand at 468.441 kb on + strand at 468.441 kb on - strand at 468.492 kb on - strand at 468.550 kb on + strand at 468.550 kb on + strand at 468.553 kb on - strand at 468.589 kb on + strand, within purD at 468.636 kb on - strand, within purD at 468.682 kb on + strand, within purD at 468.779 kb on + strand, within purD at 468.785 kb on - strand, within purD at 468.899 kb on + strand, within purD at 468.900 kb on - strand, within purD at 468.915 kb on - strand, within purD at 468.915 kb on - strand, within purD at 468.951 kb on + strand, within purD at 469.016 kb on - strand, within purD
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 464,053 + SO0438 0.24 +1.2 464,149 + SO0438 0.36 +1.5 464,258 + SO0438 0.50 +0.5 464,260 + SO0438 0.50 -1.1 464,291 + SO0438 0.54 -0.5 464,299 - SO0438 0.55 -1.8 464,299 - SO0438 0.55 -0.6 464,299 - SO0438 0.55 -0.1 464,299 - SO0438 0.55 +1.3 464,456 + SO0438 0.75 -0.2 464,481 + SO0438 0.78 -0.1 464,511 - SO0438 0.81 +0.9 464,593 + -0.7 464,601 - -0.0 464,601 - -0.5 464,646 + +0.2 464,914 + +0.0 464,916 + -0.2 464,946 + -0.5 465,059 - -0.9 465,082 - +0.3 465,107 + -0.5 465,107 + -0.3 465,108 + -0.6 465,108 + +0.1 465,116 - +1.0 465,116 - -0.4 465,175 + -0.6 465,228 - -0.9 465,273 - +0.3 465,316 - -1.0 465,507 + SO0440 0.15 +0.6 465,549 + SO0440 0.16 -0.4 465,649 - SO0440 0.19 -0.2 465,758 - SO0440 0.23 +0.0 465,777 + SO0440 0.23 -0.5 465,813 + SO0440 0.24 +0.9 465,813 + SO0440 0.24 -0.2 465,813 + SO0440 0.24 -0.1 465,890 - SO0440 0.27 -0.7 465,948 - SO0440 0.28 -0.1 465,948 - SO0440 0.28 -0.5 466,052 + SO0440 0.32 -0.2 466,128 + SO0440 0.34 -1.1 466,128 + SO0440 0.34 -0.4 466,128 + SO0440 0.34 -0.8 466,128 + SO0440 0.34 -0.6 466,136 - SO0440 0.34 +0.2 466,136 - SO0440 0.34 -0.5 466,136 - SO0440 0.34 -1.5 466,136 - SO0440 0.34 -0.6 466,136 - SO0440 0.34 -0.1 466,136 - SO0440 0.34 -0.1 466,136 - SO0440 0.34 -0.7 466,138 + SO0440 0.34 -1.4 466,162 + SO0440 0.35 +0.6 466,162 + SO0440 0.35 +0.2 466,170 - SO0440 0.35 +0.0 466,264 - SO0440 0.38 -0.3 466,419 + SO0440 0.43 -0.3 466,420 + SO0440 0.43 +1.4 466,427 - SO0440 0.43 -0.6 466,427 - SO0440 0.43 +1.0 466,427 - SO0440 0.43 -0.2 466,475 + SO0440 0.45 +0.3 466,475 + SO0440 0.45 -1.1 466,525 + SO0440 0.46 -0.6 466,650 - SO0440 0.50 -0.2 466,650 - SO0440 0.50 -0.6 466,678 - SO0440 0.51 -0.0 466,689 + SO0440 0.51 -0.3 466,763 + SO0440 0.54 +2.0 466,763 + SO0440 0.54 +0.1 466,771 - SO0440 0.54 -1.3 466,771 - SO0440 0.54 -0.3 466,771 - SO0440 0.54 -0.1 466,888 + SO0440 0.58 +0.8 466,930 - SO0440 0.59 +0.1 466,945 - SO0440 0.59 -0.5 466,986 - SO0440 0.61 -0.4 466,994 - SO0440 0.61 +0.6 467,045 - SO0440 0.63 -1.4 467,152 + SO0440 0.66 +0.4 467,236 + SO0440 0.68 -0.4 467,263 + SO0440 0.69 -0.4 467,271 - SO0440 0.70 -0.5 467,271 - SO0440 0.70 -0.3 467,324 - SO0440 0.71 -0.1 467,508 + SO0440 0.77 -1.7 467,527 + SO0440 0.77 +0.0 467,533 - SO0440 0.78 +1.3 467,545 - SO0440 0.78 +0.1 467,575 + SO0440 0.79 +0.2 467,625 + SO0440 0.81 +0.0 467,651 + SO0440 0.81 +0.8 467,669 + SO0440 0.82 -0.3 467,669 - SO0440 0.82 -0.1 467,788 + SO0440 0.86 +0.2 467,796 - SO0440 0.86 +0.1 467,845 - SO0440 0.87 -0.2 467,879 + SO0440 0.88 -1.2 467,930 + +0.6 468,060 - -0.9 468,157 + -0.2 468,167 - -0.5 468,175 - -0.8 468,204 - +0.7 468,219 + +0.2 468,421 + -0.5 468,429 - +0.6 468,441 + -1.8 468,441 - -2.4 468,492 - -1.9 468,550 + -0.7 468,550 + -1.9 468,553 - -4.4 468,589 + purD SO0441 0.12 -1.7 468,636 - purD SO0441 0.16 -3.9 468,682 + purD SO0441 0.19 -0.8 468,779 + purD SO0441 0.27 -3.2 468,785 - purD SO0441 0.27 -1.5 468,899 + purD SO0441 0.36 -2.3 468,900 - purD SO0441 0.36 -2.1 468,915 - purD SO0441 0.37 -4.5 468,915 - purD SO0441 0.37 -1.6 468,951 + purD SO0441 0.40 -1.2 469,016 - purD SO0441 0.45 -0.8
Or see this region's nucleotide sequence