Strain Fitness in Shewanella oneidensis MR-1 around SO_t056

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2744 and SO2745 are separated by 60 nucleotidesSO2745 and tRNA-Lys-7 are separated by 530 nucleotidestRNA-Lys-7 and tRNA-Lys-6 are separated by 33 nucleotidestRNA-Lys-6 and tRNA-Lys-5 are separated by 161 nucleotidestRNA-Lys-5 and tRNA-Lys-4 are separated by 33 nucleotidestRNA-Lys-4 and tRNA-Lys-3 are separated by 33 nucleotidestRNA-Lys-3 and tRNA-Lys-8 are separated by 33 nucleotidestRNA-Lys-8 and tRNA-Lys-2 are separated by 33 nucleotidestRNA-Lys-2 and tRNA-Lys-1 are separated by 54 nucleotidestRNA-Lys-1 and SO2746 are separated by 142 nucleotides SO2744: SO2744 - DNA damage response helicase (yejH or radD) (from data), at 2,864,065 to 2,865,837 SO2744 SO2745: SO2745 - glutaredoxin (NCBI ptt file), at 2,865,898 to 2,866,158 SO2745 SO_t055: tRNA-Lys-7 - tRNA-Lys (RefSeq), at 2,866,689 to 2,866,764 tRNA-Lys-7 SO_t056: tRNA-Lys-6 - tRNA-Lys (RefSeq), at 2,866,798 to 2,866,873 tRNA-Lys-6 SO_t057: tRNA-Lys-5 - tRNA-Lys (RefSeq), at 2,867,035 to 2,867,110 tRNA-Lys-5 SO_t058: tRNA-Lys-4 - tRNA-Lys (RefSeq), at 2,867,144 to 2,867,219 tRNA-Lys-4 SO_t059: tRNA-Lys-3 - tRNA-Lys (RefSeq), at 2,867,253 to 2,867,328 tRNA-Lys-3 SO_t060: tRNA-Lys-8 - tRNA-Lys (RefSeq), at 2,867,362 to 2,867,452 tRNA-Lys-8 SO_t061: tRNA-Lys-2 - tRNA-Lys (RefSeq), at 2,867,486 to 2,867,561 tRNA-Lys-2 SO_t062: tRNA-Lys-1 - tRNA-Lys (RefSeq), at 2,867,616 to 2,867,691 tRNA-Lys-1 SO2746: SO2746 - conserved hypothetical protein (NCBI ptt file), at 2,867,834 to 2,868,586 SO2746 Position (kb) 2866 2867Strain fitness (log2 ratio) -1 0 1at 2865.831 kb on + strandat 2865.839 kb on - strandat 2865.863 kb on + strandat 2865.892 kb on + strandat 2865.930 kb on + strand, within SO2745at 2865.938 kb on - strand, within SO2745at 2865.947 kb on + strand, within SO2745at 2865.947 kb on + strand, within SO2745at 2865.949 kb on + strand, within SO2745at 2865.949 kb on + strand, within SO2745at 2865.955 kb on - strand, within SO2745at 2866.011 kb on - strand, within SO2745at 2866.120 kb on - strand, within SO2745at 2866.189 kb on - strandat 2866.480 kb on + strandat 2867.863 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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2,865,831 + +0.1
2,865,839 - -0.5
2,865,863 + +0.2
2,865,892 + +0.3
2,865,930 + SO2745 0.12 -0.1
2,865,938 - SO2745 0.15 -0.0
2,865,947 + SO2745 0.19 -0.2
2,865,947 + SO2745 0.19 +1.5
2,865,949 + SO2745 0.20 +1.5
2,865,949 + SO2745 0.20 -0.6
2,865,955 - SO2745 0.22 +0.0
2,866,011 - SO2745 0.43 -0.1
2,866,120 - SO2745 0.85 +0.6
2,866,189 - -0.7
2,866,480 + -0.0
2,867,863 + +0.7

Or see this region's nucleotide sequence