Strain Fitness in Shewanella oneidensis MR-1 around SO3220

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliP and fliO overlap by 1 nucleotidesfliO and fliN are separated by 34 nucleotidesfliN and fliM are separated by 12 nucleotides SO3218: fliP - flagellar biosynthetic protein FliP (NCBI ptt file), at 3,357,856 to 3,358,599 fliP SO3219: fliO - flagellar protein FliO (NCBI ptt file), at 3,358,599 to 3,359,006 fliO SO3220: fliN - flagellar motor switch protein FliN (NCBI ptt file), at 3,359,041 to 3,359,415 fliN SO3221: fliM - flagellar motor switch protein FliM (NCBI ptt file), at 3,359,428 to 3,360,459 fliM Position (kb) 3359 3360Strain fitness (log2 ratio) -2 -1 0 1at 3358.088 kb on - strand, within fliPat 3358.152 kb on + strand, within fliPat 3358.196 kb on + strand, within fliPat 3358.204 kb on - strand, within fliPat 3358.249 kb on - strand, within fliPat 3358.319 kb on - strand, within fliPat 3358.321 kb on + strand, within fliPat 3358.436 kb on - strand, within fliPat 3358.485 kb on - strand, within fliPat 3358.565 kb on + strandat 3358.594 kb on - strandat 3358.619 kb on + strandat 3358.757 kb on + strand, within fliOat 3358.757 kb on + strand, within fliOat 3358.861 kb on + strand, within fliOat 3358.962 kb on + strand, within fliOat 3359.028 kb on - strandat 3359.074 kb on + strandat 3359.082 kb on - strand, within fliNat 3359.157 kb on + strand, within fliNat 3359.178 kb on + strand, within fliNat 3359.180 kb on + strand, within fliNat 3359.299 kb on - strand, within fliNat 3359.300 kb on + strand, within fliNat 3359.327 kb on + strand, within fliNat 3359.336 kb on + strand, within fliNat 3359.401 kb on - strandat 3359.408 kb on + strandat 3359.518 kb on + strandat 3359.684 kb on - strand, within fliMat 3359.732 kb on - strand, within fliMat 3359.742 kb on - strand, within fliMat 3359.829 kb on - strand, within fliMat 3359.894 kb on + strand, within fliMat 3359.909 kb on + strand, within fliMat 3359.947 kb on + strand, within fliMat 3359.955 kb on - strand, within fliMat 3359.966 kb on + strand, within fliMat 3359.974 kb on - strand, within fliMat 3360.006 kb on - strand, within fliMat 3360.011 kb on - strand, within fliMat 3360.157 kb on + strand, within fliMat 3360.237 kb on - strand, within fliMat 3360.327 kb on + strand, within fliMat 3360.415 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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3,358,088 - fliP SO3218 0.31 -0.2
3,358,152 + fliP SO3218 0.40 -0.7
3,358,196 + fliP SO3218 0.46 -0.6
3,358,204 - fliP SO3218 0.47 +1.2
3,358,249 - fliP SO3218 0.53 +0.6
3,358,319 - fliP SO3218 0.62 -0.1
3,358,321 + fliP SO3218 0.62 -1.2
3,358,436 - fliP SO3218 0.78 +0.0
3,358,485 - fliP SO3218 0.85 -1.1
3,358,565 + +0.1
3,358,594 - -1.4
3,358,619 + -0.7
3,358,757 + fliO SO3219 0.39 -0.4
3,358,757 + fliO SO3219 0.39 -1.1
3,358,861 + fliO SO3219 0.64 -0.6
3,358,962 + fliO SO3219 0.89 -0.3
3,359,028 - -0.2
3,359,074 + -0.6
3,359,082 - fliN SO3220 0.11 -0.2
3,359,157 + fliN SO3220 0.31 +0.9
3,359,178 + fliN SO3220 0.37 -2.0
3,359,180 + fliN SO3220 0.37 +0.3
3,359,299 - fliN SO3220 0.69 +0.1
3,359,300 + fliN SO3220 0.69 +0.3
3,359,327 + fliN SO3220 0.76 +0.2
3,359,336 + fliN SO3220 0.79 -1.2
3,359,401 - -0.1
3,359,408 + +0.3
3,359,518 + +0.8
3,359,684 - fliM SO3221 0.25 -1.4
3,359,732 - fliM SO3221 0.29 -0.9
3,359,742 - fliM SO3221 0.30 -0.4
3,359,829 - fliM SO3221 0.39 +0.5
3,359,894 + fliM SO3221 0.45 -0.6
3,359,909 + fliM SO3221 0.47 +0.5
3,359,947 + fliM SO3221 0.50 -0.5
3,359,955 - fliM SO3221 0.51 -0.1
3,359,966 + fliM SO3221 0.52 -0.7
3,359,974 - fliM SO3221 0.53 -0.5
3,360,006 - fliM SO3221 0.56 -0.7
3,360,011 - fliM SO3221 0.56 +0.3
3,360,157 + fliM SO3221 0.71 -1.4
3,360,237 - fliM SO3221 0.78 -0.6
3,360,327 + fliM SO3221 0.87 +0.4
3,360,415 + +0.7

Or see this region's nucleotide sequence