Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recQ and SO4242 are separated by 251 nucleotides SO4242 and rarD are separated by 216 nucleotides rarD and SO4244 are separated by 43 nucleotides SO4244 and argA are separated by 104 nucleotides
SO4241: recQ - ATP-dependent DNA helicase RecQ (NCBI ptt file), at 4,418,789 to 4,420,612
recQ
SO4242: SO4242 - conserved hypothetical protein (NCBI ptt file), at 4,420,864 to 4,421,328
SO4242
SO4243: rarD - rarD protein (NCBI ptt file), at 4,421,545 to 4,422,429
rarD
SO4244: SO4244 - hypothetical protein (NCBI ptt file), at 4,422,473 to 4,422,991
SO4244
SO4245: argA - amino-acid acetyltransferase (NCBI ptt file), at 4,423,096 to 4,424,433
argA
Position (kb)
4421
4422
4423 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4420.561 kb on - strand at 4420.667 kb on + strand at 4420.675 kb on - strand at 4420.675 kb on - strand at 4420.734 kb on + strand at 4420.765 kb on + strand at 4420.780 kb on - strand at 4420.799 kb on - strand at 4420.806 kb on + strand at 4420.814 kb on - strand at 4420.814 kb on - strand at 4420.879 kb on - strand at 4420.896 kb on - strand at 4420.920 kb on + strand, within SO4242 at 4420.928 kb on - strand, within SO4242 at 4420.976 kb on + strand, within SO4242 at 4420.984 kb on - strand, within SO4242 at 4420.984 kb on - strand, within SO4242 at 4421.061 kb on + strand, within SO4242 at 4421.064 kb on - strand, within SO4242 at 4421.067 kb on - strand, within SO4242 at 4421.069 kb on - strand, within SO4242 at 4421.153 kb on + strand, within SO4242 at 4421.161 kb on - strand, within SO4242 at 4421.187 kb on - strand, within SO4242 at 4421.308 kb on + strand at 4421.312 kb on + strand at 4421.320 kb on - strand at 4421.320 kb on - strand at 4421.467 kb on + strand at 4421.496 kb on - strand at 4421.498 kb on + strand at 4421.513 kb on - strand at 4421.555 kb on - strand at 4421.560 kb on - strand at 4421.583 kb on + strand at 4421.598 kb on - strand at 4421.740 kb on + strand, within rarD at 4421.824 kb on + strand, within rarD at 4421.851 kb on + strand, within rarD at 4421.859 kb on - strand, within rarD at 4421.933 kb on + strand, within rarD at 4421.944 kb on + strand, within rarD at 4421.944 kb on + strand, within rarD at 4421.952 kb on - strand, within rarD at 4421.954 kb on + strand, within rarD at 4422.023 kb on + strand, within rarD at 4422.031 kb on - strand, within rarD at 4422.031 kb on - strand, within rarD at 4422.031 kb on - strand, within rarD at 4422.054 kb on + strand, within rarD at 4422.100 kb on - strand, within rarD at 4422.113 kb on + strand, within rarD at 4422.133 kb on + strand, within rarD at 4422.141 kb on - strand, within rarD at 4422.141 kb on - strand, within rarD at 4422.141 kb on - strand, within rarD at 4422.233 kb on - strand, within rarD at 4422.314 kb on + strand, within rarD at 4422.390 kb on - strand at 4422.390 kb on - strand at 4422.395 kb on + strand at 4422.562 kb on - strand, within SO4244 at 4422.607 kb on + strand, within SO4244 at 4422.615 kb on - strand, within SO4244 at 4422.615 kb on - strand, within SO4244 at 4422.615 kb on - strand, within SO4244 at 4422.769 kb on + strand, within SO4244 at 4422.771 kb on + strand, within SO4244 at 4422.776 kb on + strand, within SO4244 at 4422.782 kb on + strand, within SO4244 at 4422.800 kb on - strand, within SO4244 at 4422.827 kb on + strand, within SO4244 at 4422.883 kb on + strand, within SO4244 at 4422.883 kb on + strand, within SO4244 at 4422.883 kb on + strand, within SO4244 at 4422.883 kb on + strand, within SO4244 at 4422.891 kb on - strand, within SO4244 at 4422.891 kb on - strand, within SO4244 at 4422.958 kb on - strand at 4423.000 kb on + strand at 4423.023 kb on - strand at 4423.023 kb on - strand at 4423.101 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 4,420,561 - +0.1 4,420,667 + -1.1 4,420,675 - -0.3 4,420,675 - -0.1 4,420,734 + +0.8 4,420,765 + -1.9 4,420,780 - +1.1 4,420,799 - +0.3 4,420,806 + +1.2 4,420,814 - +0.1 4,420,814 - -0.6 4,420,879 - +0.6 4,420,896 - +0.3 4,420,920 + SO4242 0.12 +0.5 4,420,928 - SO4242 0.14 -1.2 4,420,976 + SO4242 0.24 +0.8 4,420,984 - SO4242 0.26 -0.6 4,420,984 - SO4242 0.26 -0.5 4,421,061 + SO4242 0.42 -0.9 4,421,064 - SO4242 0.43 +1.1 4,421,067 - SO4242 0.44 -0.0 4,421,069 - SO4242 0.44 +0.5 4,421,153 + SO4242 0.62 +0.4 4,421,161 - SO4242 0.64 -1.0 4,421,187 - SO4242 0.69 +0.6 4,421,308 + +0.0 4,421,312 + -0.4 4,421,320 - -0.4 4,421,320 - +0.2 4,421,467 + -0.2 4,421,496 - +0.3 4,421,498 + -1.0 4,421,513 - +0.8 4,421,555 - +2.4 4,421,560 - +0.6 4,421,583 + +0.6 4,421,598 - +0.7 4,421,740 + rarD SO4243 0.22 -0.5 4,421,824 + rarD SO4243 0.32 -0.1 4,421,851 + rarD SO4243 0.35 -0.3 4,421,859 - rarD SO4243 0.35 -0.2 4,421,933 + rarD SO4243 0.44 -0.7 4,421,944 + rarD SO4243 0.45 +0.3 4,421,944 + rarD SO4243 0.45 +0.1 4,421,952 - rarD SO4243 0.46 +1.3 4,421,954 + rarD SO4243 0.46 +1.2 4,422,023 + rarD SO4243 0.54 -0.2 4,422,031 - rarD SO4243 0.55 +1.0 4,422,031 - rarD SO4243 0.55 +0.4 4,422,031 - rarD SO4243 0.55 +0.7 4,422,054 + rarD SO4243 0.58 +2.3 4,422,100 - rarD SO4243 0.63 +0.4 4,422,113 + rarD SO4243 0.64 +0.6 4,422,133 + rarD SO4243 0.66 +0.6 4,422,141 - rarD SO4243 0.67 -0.1 4,422,141 - rarD SO4243 0.67 +0.3 4,422,141 - rarD SO4243 0.67 +0.9 4,422,233 - rarD SO4243 0.78 +0.7 4,422,314 + rarD SO4243 0.87 +1.0 4,422,390 - +0.9 4,422,390 - +0.9 4,422,395 + +0.1 4,422,562 - SO4244 0.17 -0.5 4,422,607 + SO4244 0.26 +0.9 4,422,615 - SO4244 0.27 -1.0 4,422,615 - SO4244 0.27 -1.0 4,422,615 - SO4244 0.27 -0.7 4,422,769 + SO4244 0.57 +0.1 4,422,771 + SO4244 0.57 -0.2 4,422,776 + SO4244 0.58 +0.2 4,422,782 + SO4244 0.60 +0.0 4,422,800 - SO4244 0.63 -0.5 4,422,827 + SO4244 0.68 +1.0 4,422,883 + SO4244 0.79 +0.6 4,422,883 + SO4244 0.79 +0.0 4,422,883 + SO4244 0.79 +1.2 4,422,883 + SO4244 0.79 +0.1 4,422,891 - SO4244 0.81 -1.3 4,422,891 - SO4244 0.81 +1.5 4,422,958 - +1.0 4,423,000 + +0.6 4,423,023 - -0.2 4,423,023 - -0.1 4,423,101 + -3.0
Or see this region's nucleotide sequence