Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metB and metJ are separated by 203 nucleotides metJ and SO4058 are separated by 96 nucleotides SO4058 and psrC are separated by 151 nucleotides
SO4056: metB - cystathionine gamma-synthase (NCBI ptt file), at 4,211,241 to 4,212,422
metB
SO4057: metJ - met repressor (NCBI ptt file), at 4,212,626 to 4,212,940
metJ
SO4058: SO4058 - conserved hypothetical protein (NCBI ptt file), at 4,213,037 to 4,213,780
SO4058
SO4060: psrC - polysulfide reductase, subunit C (NCBI ptt file), at 4,213,932 to 4,214,876
psrC
Position (kb) 4213
4214 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4212.186 kb on - strand, within metB at 4212.274 kb on - strand, within metB at 4212.575 kb on + strand at 4212.622 kb on + strand at 4212.663 kb on - strand, within metJ at 4212.704 kb on + strand, within metJ at 4212.768 kb on - strand, within metJ at 4212.840 kb on - strand, within metJ at 4212.840 kb on - strand, within metJ at 4213.074 kb on + strand at 4213.127 kb on - strand, within SO4058 at 4213.316 kb on - strand, within SO4058 at 4213.334 kb on + strand, within SO4058 at 4213.423 kb on + strand, within SO4058 at 4213.609 kb on + strand, within SO4058 at 4213.617 kb on - strand, within SO4058 at 4213.710 kb on + strand at 4213.710 kb on + strand at 4213.710 kb on + strand at 4213.718 kb on - strand at 4213.735 kb on - strand at 4213.944 kb on + strand at 4213.952 kb on - strand at 4213.959 kb on + strand at 4213.968 kb on + strand at 4214.007 kb on - strand at 4214.023 kb on + strand at 4214.103 kb on + strand, within psrC at 4214.120 kb on + strand, within psrC at 4214.120 kb on + strand, within psrC at 4214.120 kb on + strand, within psrC at 4214.120 kb on + strand, within psrC at 4214.128 kb on - strand, within psrC at 4214.128 kb on - strand, within psrC at 4214.184 kb on - strand, within psrC at 4214.184 kb on - strand, within psrC at 4214.225 kb on + strand, within psrC at 4214.225 kb on + strand, within psrC at 4214.233 kb on - strand, within psrC at 4214.277 kb on - strand, within psrC at 4214.304 kb on - strand, within psrC at 4214.324 kb on - strand, within psrC at 4214.339 kb on + strand, within psrC at 4214.347 kb on - strand, within psrC at 4214.448 kb on - strand, within psrC at 4214.450 kb on + strand, within psrC at 4214.469 kb on - strand, within psrC at 4214.469 kb on - strand, within psrC at 4214.565 kb on - strand, within psrC at 4214.669 kb on + strand, within psrC at 4214.672 kb on - strand, within psrC at 4214.684 kb on - strand, within psrC at 4214.729 kb on - strand, within psrC
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 4,212,186 - metB SO4056 0.80 -4.7 4,212,274 - metB SO4056 0.87 -1.6 4,212,575 + +3.7 4,212,622 + +0.3 4,212,663 - metJ SO4057 0.12 -1.3 4,212,704 + metJ SO4057 0.25 -2.5 4,212,768 - metJ SO4057 0.45 -1.3 4,212,840 - metJ SO4057 0.68 -3.2 4,212,840 - metJ SO4057 0.68 -1.7 4,213,074 + -0.4 4,213,127 - SO4058 0.12 -0.5 4,213,316 - SO4058 0.38 -1.6 4,213,334 + SO4058 0.40 -2.5 4,213,423 + SO4058 0.52 -0.6 4,213,609 + SO4058 0.77 -1.1 4,213,617 - SO4058 0.78 -0.8 4,213,710 + -1.1 4,213,710 + -1.1 4,213,710 + -1.0 4,213,718 - +0.6 4,213,735 - -0.6 4,213,944 + -1.8 4,213,952 - -0.5 4,213,959 + -0.4 4,213,968 + -2.8 4,214,007 - +0.7 4,214,023 + -0.6 4,214,103 + psrC SO4060 0.18 +0.2 4,214,120 + psrC SO4060 0.20 +0.9 4,214,120 + psrC SO4060 0.20 +3.3 4,214,120 + psrC SO4060 0.20 +0.8 4,214,120 + psrC SO4060 0.20 +1.0 4,214,128 - psrC SO4060 0.21 +0.2 4,214,128 - psrC SO4060 0.21 -0.0 4,214,184 - psrC SO4060 0.27 +0.1 4,214,184 - psrC SO4060 0.27 -0.7 4,214,225 + psrC SO4060 0.31 +0.3 4,214,225 + psrC SO4060 0.31 +0.5 4,214,233 - psrC SO4060 0.32 +0.9 4,214,277 - psrC SO4060 0.37 +0.6 4,214,304 - psrC SO4060 0.39 -0.1 4,214,324 - psrC SO4060 0.41 -0.2 4,214,339 + psrC SO4060 0.43 -0.7 4,214,347 - psrC SO4060 0.44 +0.5 4,214,448 - psrC SO4060 0.55 +0.4 4,214,450 + psrC SO4060 0.55 +1.0 4,214,469 - psrC SO4060 0.57 -0.1 4,214,469 - psrC SO4060 0.57 -0.2 4,214,565 - psrC SO4060 0.67 -1.1 4,214,669 + psrC SO4060 0.78 -1.5 4,214,672 - psrC SO4060 0.78 -1.3 4,214,684 - psrC SO4060 0.80 +0.4 4,214,729 - psrC SO4060 0.84 +0.9
Or see this region's nucleotide sequence