Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt parE and SO3900 are separated by 56 nucleotides SO3900 and icc are separated by 26 nucleotides icc and SO3902 are separated by 15 nucleotides SO3902 and SO3903 are separated by 13 nucleotides
SO3899: parE - DNA topoisomerase IV, B subunit (NCBI ptt file), at 4,046,861 to 4,048,747
parE
SO3900: SO3900 - yqiA-like hydrolase, affects the cell envelope (from data) , at 4,048,804 to 4,049,379
SO3900
SO3901: icc - lacZ expression regulator (NCBI ptt file), at 4,049,406 to 4,050,242
icc
SO3902: SO3902 - conserved hypothetical protein (NCBI ptt file), at 4,050,258 to 4,050,698
SO3902
SO3903: SO3903 - MutT/nudix family protein (NCBI ptt file), at 4,050,712 to 4,051,314
SO3903
Position (kb)
4049
4050
4051 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4048.832 kb on - strand at 4048.904 kb on + strand, within SO3900 at 4049.020 kb on - strand, within SO3900 at 4049.130 kb on - strand, within SO3900 at 4049.140 kb on - strand, within SO3900 at 4049.140 kb on - strand, within SO3900 at 4049.220 kb on + strand, within SO3900 at 4049.257 kb on - strand, within SO3900 at 4049.366 kb on + strand at 4049.465 kb on - strand at 4049.470 kb on - strand at 4049.470 kb on - strand at 4049.470 kb on - strand at 4049.485 kb on + strand at 4049.493 kb on - strand, within icc at 4049.493 kb on - strand, within icc at 4049.565 kb on + strand, within icc at 4049.565 kb on + strand, within icc at 4049.573 kb on - strand, within icc at 4049.661 kb on - strand, within icc at 4049.661 kb on - strand, within icc at 4049.698 kb on + strand, within icc at 4049.712 kb on + strand, within icc at 4049.712 kb on + strand, within icc at 4049.720 kb on - strand, within icc at 4049.720 kb on - strand, within icc at 4049.720 kb on - strand, within icc at 4050.191 kb on - strand at 4050.263 kb on - strand at 4050.286 kb on - strand at 4050.348 kb on - strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.366 kb on + strand, within SO3902 at 4050.374 kb on - strand, within SO3902 at 4050.374 kb on - strand, within SO3902 at 4050.374 kb on - strand, within SO3902 at 4050.374 kb on - strand, within SO3902 at 4050.374 kb on - strand, within SO3902 at 4050.374 kb on - strand, within SO3902 at 4050.374 kb on - strand, within SO3902 at 4050.374 kb on - strand, within SO3902 at 4050.374 kb on - strand, within SO3902 at 4050.374 kb on - strand, within SO3902 at 4050.374 kb on - strand, within SO3902 at 4050.447 kb on + strand, within SO3902 at 4050.449 kb on + strand, within SO3902 at 4050.449 kb on + strand, within SO3902 at 4050.690 kb on - strand at 4050.784 kb on + strand, within SO3903 at 4050.806 kb on + strand, within SO3903 at 4050.814 kb on + strand, within SO3903 at 4050.864 kb on + strand, within SO3903 at 4050.867 kb on - strand, within SO3903 at 4050.920 kb on - strand, within SO3903 at 4050.930 kb on - strand, within SO3903 at 4050.930 kb on - strand, within SO3903 at 4050.938 kb on + strand, within SO3903 at 4050.966 kb on - strand, within SO3903 at 4050.992 kb on + strand, within SO3903 at 4051.053 kb on + strand, within SO3903 at 4051.096 kb on - strand, within SO3903 at 4051.132 kb on - strand, within SO3903 at 4051.132 kb on - strand, within SO3903 at 4051.146 kb on - strand, within SO3903 at 4051.195 kb on - strand, within SO3903 at 4051.218 kb on + strand, within SO3903
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 4,048,832 - +1.5 4,048,904 + SO3900 0.17 +2.8 4,049,020 - SO3900 0.38 +0.3 4,049,130 - SO3900 0.57 +1.1 4,049,140 - SO3900 0.58 +3.1 4,049,140 - SO3900 0.58 +2.1 4,049,220 + SO3900 0.72 +0.5 4,049,257 - SO3900 0.79 +0.7 4,049,366 + +0.7 4,049,465 - -2.5 4,049,470 - -3.0 4,049,470 - -4.4 4,049,470 - -3.4 4,049,485 + -2.2 4,049,493 - icc SO3901 0.10 -2.0 4,049,493 - icc SO3901 0.10 -0.3 4,049,565 + icc SO3901 0.19 -1.5 4,049,565 + icc SO3901 0.19 -1.8 4,049,573 - icc SO3901 0.20 -1.5 4,049,661 - icc SO3901 0.30 -1.3 4,049,661 - icc SO3901 0.30 -1.6 4,049,698 + icc SO3901 0.35 -2.6 4,049,712 + icc SO3901 0.37 -2.7 4,049,712 + icc SO3901 0.37 -3.1 4,049,720 - icc SO3901 0.38 -2.2 4,049,720 - icc SO3901 0.38 -1.9 4,049,720 - icc SO3901 0.38 +0.7 4,050,191 - -2.5 4,050,263 - -2.0 4,050,286 - -0.3 4,050,348 - SO3902 0.20 +1.5 4,050,366 + SO3902 0.24 -3.1 4,050,366 + SO3902 0.24 -4.1 4,050,366 + SO3902 0.24 -3.5 4,050,366 + SO3902 0.24 -1.9 4,050,366 + SO3902 0.24 -4.3 4,050,366 + SO3902 0.24 -3.1 4,050,366 + SO3902 0.24 -1.6 4,050,366 + SO3902 0.24 -1.6 4,050,366 + SO3902 0.24 -1.5 4,050,366 + SO3902 0.24 -0.3 4,050,366 + SO3902 0.24 -3.7 4,050,366 + SO3902 0.24 -3.3 4,050,374 - SO3902 0.26 -2.1 4,050,374 - SO3902 0.26 -1.8 4,050,374 - SO3902 0.26 -1.5 4,050,374 - SO3902 0.26 -2.6 4,050,374 - SO3902 0.26 -0.9 4,050,374 - SO3902 0.26 -2.3 4,050,374 - SO3902 0.26 -1.3 4,050,374 - SO3902 0.26 -2.0 4,050,374 - SO3902 0.26 -1.0 4,050,374 - SO3902 0.26 +0.4 4,050,374 - SO3902 0.26 -0.5 4,050,447 + SO3902 0.43 -1.1 4,050,449 + SO3902 0.43 -0.2 4,050,449 + SO3902 0.43 +0.3 4,050,690 - +0.4 4,050,784 + SO3903 0.12 +1.0 4,050,806 + SO3903 0.16 -0.3 4,050,814 + SO3903 0.17 -1.2 4,050,864 + SO3903 0.25 +0.9 4,050,867 - SO3903 0.26 +0.2 4,050,920 - SO3903 0.34 -3.5 4,050,930 - SO3903 0.36 +2.0 4,050,930 - SO3903 0.36 +0.9 4,050,938 + SO3903 0.37 -0.5 4,050,966 - SO3903 0.42 +0.0 4,050,992 + SO3903 0.46 +0.0 4,051,053 + SO3903 0.57 -1.7 4,051,096 - SO3903 0.64 -0.2 4,051,132 - SO3903 0.70 -0.7 4,051,132 - SO3903 0.70 +0.2 4,051,146 - SO3903 0.72 -0.4 4,051,195 - SO3903 0.80 -1.9 4,051,218 + SO3903 0.84 -1.2
Or see this region's nucleotide sequence