Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lolB and ispE are separated by 1 nucleotides ispE and prsA are separated by 112 nucleotides prsA and SO3838 are separated by 73 nucleotides
SO3835: lolB - outer membrane lipoprotein LolB (NCBI ptt file), at 3,985,011 to 3,985,655
lolB
SO3836: ispE - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase (NCBI ptt file), at 3,985,657 to 3,986,511
ispE
SO3837: prsA - ribose-phosphate pyrophosphokinase (NCBI ptt file), at 3,986,624 to 3,987,571
prsA
SO3838: SO3838 - methyl-accepting chemotaxis protein (NCBI ptt file), at 3,987,645 to 3,989,507
SO3838
Position (kb)
3986
3987
3988 Strain fitness (log2 ratio)
-1
0
1
2 at 3985.627 kb on + strand at 3987.797 kb on + strand at 3987.862 kb on - strand, within SO3838 at 3987.879 kb on + strand, within SO3838 at 3987.934 kb on + strand, within SO3838 at 3988.019 kb on + strand, within SO3838 at 3988.027 kb on - strand, within SO3838 at 3988.056 kb on + strand, within SO3838 at 3988.057 kb on + strand, within SO3838 at 3988.145 kb on - strand, within SO3838 at 3988.182 kb on - strand, within SO3838 at 3988.190 kb on + strand, within SO3838 at 3988.222 kb on - strand, within SO3838 at 3988.265 kb on - strand, within SO3838 at 3988.265 kb on - strand, within SO3838 at 3988.356 kb on + strand, within SO3838 at 3988.363 kb on + strand, within SO3838 at 3988.388 kb on + strand, within SO3838 at 3988.563 kb on + strand, within SO3838 at 3988.571 kb on - strand, within SO3838 at 3988.571 kb on - strand, within SO3838 at 3988.571 kb on - strand, within SO3838 at 3988.571 kb on - strand, within SO3838
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 3,985,627 + -0.0 3,987,797 + -0.3 3,987,862 - SO3838 0.12 -0.4 3,987,879 + SO3838 0.13 -0.2 3,987,934 + SO3838 0.16 -0.9 3,988,019 + SO3838 0.20 +0.5 3,988,027 - SO3838 0.21 -1.1 3,988,056 + SO3838 0.22 -0.7 3,988,057 + SO3838 0.22 -0.4 3,988,145 - SO3838 0.27 +2.1 3,988,182 - SO3838 0.29 -1.4 3,988,190 + SO3838 0.29 +0.1 3,988,222 - SO3838 0.31 -1.5 3,988,265 - SO3838 0.33 +0.2 3,988,265 - SO3838 0.33 -0.6 3,988,356 + SO3838 0.38 +0.4 3,988,363 + SO3838 0.39 -1.0 3,988,388 + SO3838 0.40 +0.5 3,988,563 + SO3838 0.49 -0.4 3,988,571 - SO3838 0.50 +0.2 3,988,571 - SO3838 0.50 -1.3 3,988,571 - SO3838 0.50 -1.5 3,988,571 - SO3838 0.50 -0.5
Or see this region's nucleotide sequence