Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ung and SO3655 are separated by 169 nucleotides SO3655 and SO3656 are separated by 70 nucleotides SO3656 and SO3657 are separated by 86 nucleotides
SO3654: ung - uracil-DNA glycosylase (NCBI ptt file), at 3,808,971 to 3,809,627
ung
SO3655: SO3655 - pilin, putative (NCBI ptt file), at 3,809,797 to 3,810,357
SO3655
SO3656: SO3656 - hypothetical protein (NCBI ptt file), at 3,810,428 to 3,810,745
SO3656
SO3657: SO3657 - transporter, LysE family (NCBI ptt file), at 3,810,832 to 3,811,521
SO3657
Position (kb)
3810
3811 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3809.429 kb on + strand, within ung at 3809.437 kb on - strand, within ung at 3809.437 kb on - strand, within ung at 3809.437 kb on - strand, within ung at 3809.437 kb on - strand, within ung at 3809.457 kb on + strand, within ung at 3809.465 kb on - strand, within ung at 3809.491 kb on + strand, within ung at 3809.505 kb on + strand, within ung at 3809.532 kb on - strand, within ung at 3809.581 kb on - strand at 3809.581 kb on - strand at 3809.615 kb on - strand at 3809.617 kb on + strand at 3809.713 kb on - strand at 3809.764 kb on - strand at 3809.847 kb on - strand at 3809.877 kb on + strand, within SO3655 at 3809.893 kb on + strand, within SO3655 at 3810.017 kb on + strand, within SO3655 at 3810.045 kb on + strand, within SO3655 at 3810.105 kb on - strand, within SO3655 at 3810.131 kb on - strand, within SO3655 at 3810.215 kb on - strand, within SO3655 at 3810.234 kb on + strand, within SO3655 at 3810.242 kb on - strand, within SO3655 at 3810.257 kb on - strand, within SO3655 at 3810.262 kb on - strand, within SO3655 at 3810.262 kb on - strand, within SO3655 at 3810.262 kb on - strand, within SO3655 at 3810.262 kb on - strand, within SO3655 at 3810.423 kb on + strand at 3810.500 kb on - strand, within SO3656 at 3810.517 kb on + strand, within SO3656 at 3810.517 kb on + strand, within SO3656 at 3810.517 kb on + strand, within SO3656 at 3810.682 kb on + strand, within SO3656 at 3810.705 kb on + strand, within SO3656 at 3810.711 kb on - strand, within SO3656 at 3810.831 kb on + strand at 3810.831 kb on + strand at 3810.839 kb on - strand at 3810.839 kb on - strand at 3810.839 kb on - strand at 3810.943 kb on + strand, within SO3657 at 3810.945 kb on - strand, within SO3657 at 3810.945 kb on - strand, within SO3657 at 3810.959 kb on - strand, within SO3657 at 3810.989 kb on - strand, within SO3657 at 3811.039 kb on - strand, within SO3657 at 3811.176 kb on + strand, within SO3657 at 3811.273 kb on - strand, within SO3657 at 3811.380 kb on - strand, within SO3657 at 3811.490 kb on - strand at 3811.501 kb on + strand at 3811.501 kb on + strand at 3811.509 kb on - strand at 3811.509 kb on - strand at 3811.663 kb on + strand at 3811.682 kb on + strand at 3811.714 kb on - strand at 3811.744 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 3,809,429 + ung SO3654 0.70 +0.9 3,809,437 - ung SO3654 0.71 -0.3 3,809,437 - ung SO3654 0.71 +0.0 3,809,437 - ung SO3654 0.71 +0.1 3,809,437 - ung SO3654 0.71 +0.0 3,809,457 + ung SO3654 0.74 -0.7 3,809,465 - ung SO3654 0.75 -0.4 3,809,491 + ung SO3654 0.79 +1.9 3,809,505 + ung SO3654 0.81 -0.8 3,809,532 - ung SO3654 0.85 +0.0 3,809,581 - -0.8 3,809,581 - +0.8 3,809,615 - -0.7 3,809,617 + +0.2 3,809,713 - -1.1 3,809,764 - +0.2 3,809,847 - -0.6 3,809,877 + SO3655 0.14 +1.0 3,809,893 + SO3655 0.17 +2.0 3,810,017 + SO3655 0.39 +0.5 3,810,045 + SO3655 0.44 +0.8 3,810,105 - SO3655 0.55 +1.6 3,810,131 - SO3655 0.60 +0.3 3,810,215 - SO3655 0.75 +0.1 3,810,234 + SO3655 0.78 +0.8 3,810,242 - SO3655 0.79 -0.1 3,810,257 - SO3655 0.82 +1.6 3,810,262 - SO3655 0.83 -0.0 3,810,262 - SO3655 0.83 -0.0 3,810,262 - SO3655 0.83 +0.2 3,810,262 - SO3655 0.83 +0.8 3,810,423 + +0.7 3,810,500 - SO3656 0.23 +0.1 3,810,517 + SO3656 0.28 +0.4 3,810,517 + SO3656 0.28 -0.3 3,810,517 + SO3656 0.28 -1.3 3,810,682 + SO3656 0.80 +0.0 3,810,705 + SO3656 0.87 -0.9 3,810,711 - SO3656 0.89 +4.4 3,810,831 + +1.7 3,810,831 + -1.8 3,810,839 - -0.1 3,810,839 - -0.1 3,810,839 - -2.0 3,810,943 + SO3657 0.16 -0.7 3,810,945 - SO3657 0.16 +1.6 3,810,945 - SO3657 0.16 -0.4 3,810,959 - SO3657 0.18 -0.8 3,810,989 - SO3657 0.23 -0.2 3,811,039 - SO3657 0.30 +0.5 3,811,176 + SO3657 0.50 +0.3 3,811,273 - SO3657 0.64 +0.9 3,811,380 - SO3657 0.79 +0.1 3,811,490 - +0.5 3,811,501 + +0.7 3,811,501 + +1.3 3,811,509 - -0.2 3,811,509 - -0.4 3,811,663 + +0.6 3,811,682 + +0.7 3,811,714 - +0.5 3,811,744 + -0.2
Or see this region's nucleotide sequence