Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glyA and SO3472 are separated by 190 nucleotides SO3472 and SO3473 are separated by 126 nucleotides
SO3471: glyA - serine hydroxymethyltransferase (NCBI ptt file), at 3,616,031 to 3,617,284
glyA
SO3472: SO3472 - ABC transporter, ATP-binding protein (NCBI ptt file), at 3,617,475 to 3,619,142
SO3472
SO3473: SO3473 - transcriptional regulator, LysR family (NCBI ptt file), at 3,619,269 to 3,620,192
SO3473
Position (kb)
3617
3618
3619
3620 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 3616.595 kb on + strand, within glyA at 3616.638 kb on + strand, within glyA at 3616.729 kb on + strand, within glyA at 3616.868 kb on - strand, within glyA at 3616.868 kb on - strand, within glyA at 3616.870 kb on + strand, within glyA at 3616.891 kb on - strand, within glyA at 3617.003 kb on - strand, within glyA at 3617.038 kb on - strand, within glyA at 3617.057 kb on + strand, within glyA at 3617.065 kb on - strand, within glyA at 3617.065 kb on - strand, within glyA at 3617.099 kb on - strand, within glyA at 3617.114 kb on - strand, within glyA at 3617.222 kb on + strand at 3617.225 kb on - strand at 3617.240 kb on - strand at 3617.311 kb on + strand at 3617.311 kb on + strand at 3617.336 kb on - strand at 3617.382 kb on + strand at 3617.597 kb on - strand at 3617.613 kb on + strand at 3617.674 kb on - strand, within SO3472 at 3617.676 kb on + strand, within SO3472 at 3617.787 kb on + strand, within SO3472 at 3617.821 kb on - strand, within SO3472 at 3617.850 kb on + strand, within SO3472 at 3617.910 kb on - strand, within SO3472 at 3617.970 kb on + strand, within SO3472 at 3618.104 kb on - strand, within SO3472 at 3618.243 kb on - strand, within SO3472 at 3618.326 kb on + strand, within SO3472 at 3618.326 kb on + strand, within SO3472 at 3618.333 kb on + strand, within SO3472 at 3618.462 kb on + strand, within SO3472 at 3618.475 kb on - strand, within SO3472 at 3618.500 kb on + strand, within SO3472 at 3618.610 kb on + strand, within SO3472 at 3618.618 kb on - strand, within SO3472 at 3618.661 kb on - strand, within SO3472 at 3618.705 kb on + strand, within SO3472 at 3618.713 kb on - strand, within SO3472 at 3618.866 kb on - strand, within SO3472 at 3618.931 kb on - strand, within SO3472 at 3619.005 kb on + strand at 3619.055 kb on - strand at 3619.173 kb on - strand at 3619.176 kb on - strand at 3619.271 kb on + strand at 3619.273 kb on - strand at 3619.305 kb on + strand at 3619.313 kb on + strand at 3619.315 kb on - strand at 3619.318 kb on + strand at 3619.326 kb on + strand at 3619.336 kb on - strand at 3619.364 kb on - strand, within SO3473 at 3619.422 kb on + strand, within SO3473 at 3619.620 kb on + strand, within SO3473 at 3619.627 kb on + strand, within SO3473 at 3619.647 kb on - strand, within SO3473 at 3619.688 kb on - strand, within SO3473 at 3619.814 kb on + strand, within SO3473 at 3619.865 kb on - strand, within SO3473 at 3619.917 kb on + strand, within SO3473 at 3620.016 kb on + strand, within SO3473 at 3620.016 kb on + strand, within SO3473 at 3620.021 kb on + strand, within SO3473 at 3620.035 kb on + strand, within SO3473
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 3,616,595 + glyA SO3471 0.45 -4.3 3,616,638 + glyA SO3471 0.48 -2.3 3,616,729 + glyA SO3471 0.56 -2.6 3,616,868 - glyA SO3471 0.67 -1.9 3,616,868 - glyA SO3471 0.67 -3.6 3,616,870 + glyA SO3471 0.67 -4.6 3,616,891 - glyA SO3471 0.69 -5.4 3,617,003 - glyA SO3471 0.78 -4.0 3,617,038 - glyA SO3471 0.80 -4.7 3,617,057 + glyA SO3471 0.82 -4.5 3,617,065 - glyA SO3471 0.82 -3.2 3,617,065 - glyA SO3471 0.82 -4.0 3,617,099 - glyA SO3471 0.85 -2.7 3,617,114 - glyA SO3471 0.86 -4.1 3,617,222 + -3.2 3,617,225 - -4.0 3,617,240 - -6.6 3,617,311 + -4.7 3,617,311 + -5.4 3,617,336 - -2.5 3,617,382 + +0.8 3,617,597 - +0.3 3,617,613 + +0.5 3,617,674 - SO3472 0.12 +1.0 3,617,676 + SO3472 0.12 +1.3 3,617,787 + SO3472 0.19 +1.7 3,617,821 - SO3472 0.21 +0.8 3,617,850 + SO3472 0.22 +1.1 3,617,910 - SO3472 0.26 +2.0 3,617,970 + SO3472 0.30 +1.5 3,618,104 - SO3472 0.38 +3.0 3,618,243 - SO3472 0.46 +2.1 3,618,326 + SO3472 0.51 +1.6 3,618,326 + SO3472 0.51 +1.5 3,618,333 + SO3472 0.51 +1.6 3,618,462 + SO3472 0.59 +1.8 3,618,475 - SO3472 0.60 +1.6 3,618,500 + SO3472 0.61 +1.8 3,618,610 + SO3472 0.68 +1.4 3,618,618 - SO3472 0.69 +0.2 3,618,661 - SO3472 0.71 +1.4 3,618,705 + SO3472 0.74 -0.2 3,618,713 - SO3472 0.74 +0.4 3,618,866 - SO3472 0.83 +1.2 3,618,931 - SO3472 0.87 +1.6 3,619,005 + +1.4 3,619,055 - +3.0 3,619,173 - +0.9 3,619,176 - +0.7 3,619,271 + +0.5 3,619,273 - +0.8 3,619,305 + +0.5 3,619,313 + -0.2 3,619,315 - +0.8 3,619,318 + -0.0 3,619,326 + +0.4 3,619,336 - -0.3 3,619,364 - SO3473 0.10 -1.4 3,619,422 + SO3473 0.17 -1.5 3,619,620 + SO3473 0.38 -0.5 3,619,627 + SO3473 0.39 -0.2 3,619,647 - SO3473 0.41 -0.8 3,619,688 - SO3473 0.45 -0.3 3,619,814 + SO3473 0.59 -1.3 3,619,865 - SO3473 0.65 +0.2 3,619,917 + SO3473 0.70 +0.2 3,620,016 + SO3473 0.81 -1.2 3,620,016 + SO3473 0.81 +0.4 3,620,021 + SO3473 0.81 +3.6 3,620,035 + SO3473 0.83 +0.7
Or see this region's nucleotide sequence