Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliG and fliF overlap by 8 nucleotides fliF and fliE are separated by 72 nucleotides fliE and flrC are separated by 191 nucleotides
SO3227: fliG - flagellar motor switch protein FliG (NCBI ptt file), at 3,365,531 to 3,366,577
fliG
SO3228: fliF - flagellar M-ring protein FliF (NCBI ptt file), at 3,366,570 to 3,368,231
fliF
SO3229: fliE - flagellar hook-basal body complex protein FliE (NCBI ptt file), at 3,368,304 to 3,368,642
fliE
SO3230: flrC - flagellar regulatory protein C (NCBI ptt file), at 3,368,834 to 3,370,174
flrC
Position (kb)
3366
3367
3368
3369 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3365.583 kb on + strand at 3365.644 kb on - strand, within fliG at 3365.744 kb on - strand, within fliG at 3365.777 kb on - strand, within fliG at 3365.783 kb on + strand, within fliG at 3365.791 kb on - strand, within fliG at 3365.926 kb on - strand, within fliG at 3365.972 kb on + strand, within fliG at 3365.980 kb on - strand, within fliG at 3366.027 kb on + strand, within fliG at 3366.035 kb on - strand, within fliG at 3366.266 kb on + strand, within fliG at 3366.274 kb on - strand, within fliG at 3366.292 kb on - strand, within fliG at 3366.393 kb on - strand, within fliG at 3366.430 kb on - strand, within fliG at 3366.432 kb on + strand, within fliG at 3366.439 kb on + strand, within fliG at 3366.475 kb on + strand at 3366.646 kb on + strand at 3366.654 kb on - strand at 3366.694 kb on - strand at 3366.708 kb on + strand at 3366.716 kb on - strand at 3366.716 kb on - strand at 3366.737 kb on + strand, within fliF at 3366.737 kb on + strand, within fliF at 3366.744 kb on + strand, within fliF at 3366.745 kb on - strand, within fliF at 3366.745 kb on - strand, within fliF at 3366.745 kb on - strand, within fliF at 3366.764 kb on - strand, within fliF at 3366.849 kb on - strand, within fliF at 3366.988 kb on + strand, within fliF at 3367.003 kb on - strand, within fliF at 3367.030 kb on - strand, within fliF at 3367.081 kb on - strand, within fliF at 3367.123 kb on - strand, within fliF at 3367.123 kb on - strand, within fliF at 3367.186 kb on - strand, within fliF at 3367.622 kb on + strand, within fliF at 3367.629 kb on + strand, within fliF at 3367.637 kb on - strand, within fliF at 3367.682 kb on - strand, within fliF at 3367.712 kb on + strand, within fliF at 3367.846 kb on - strand, within fliF at 3367.856 kb on - strand, within fliF at 3367.879 kb on - strand, within fliF at 3367.923 kb on + strand, within fliF at 3367.931 kb on - strand, within fliF at 3367.944 kb on - strand, within fliF at 3368.039 kb on - strand, within fliF at 3368.081 kb on + strand at 3368.207 kb on - strand at 3368.479 kb on + strand, within fliE at 3368.487 kb on - strand, within fliE at 3368.651 kb on + strand at 3368.952 kb on - strand at 3368.979 kb on + strand, within flrC at 3368.979 kb on + strand, within flrC at 3369.028 kb on + strand, within flrC at 3369.083 kb on - strand, within flrC at 3369.093 kb on - strand, within flrC at 3369.123 kb on - strand, within flrC at 3369.128 kb on - strand, within flrC at 3369.155 kb on - strand, within flrC
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 3,365,583 + +0.4 3,365,644 - fliG SO3227 0.11 +1.9 3,365,744 - fliG SO3227 0.20 +1.9 3,365,777 - fliG SO3227 0.23 +2.9 3,365,783 + fliG SO3227 0.24 +2.7 3,365,791 - fliG SO3227 0.25 +0.7 3,365,926 - fliG SO3227 0.38 +2.4 3,365,972 + fliG SO3227 0.42 +1.4 3,365,980 - fliG SO3227 0.43 +1.9 3,366,027 + fliG SO3227 0.47 +1.8 3,366,035 - fliG SO3227 0.48 +2.1 3,366,266 + fliG SO3227 0.70 +2.6 3,366,274 - fliG SO3227 0.71 +1.5 3,366,292 - fliG SO3227 0.73 +1.6 3,366,393 - fliG SO3227 0.82 +1.8 3,366,430 - fliG SO3227 0.86 +2.3 3,366,432 + fliG SO3227 0.86 +1.3 3,366,439 + fliG SO3227 0.87 +1.5 3,366,475 + +1.9 3,366,646 + +2.6 3,366,654 - +1.2 3,366,694 - +1.7 3,366,708 + +1.9 3,366,716 - +0.7 3,366,716 - +2.3 3,366,737 + fliF SO3228 0.10 +1.2 3,366,737 + fliF SO3228 0.10 +1.6 3,366,744 + fliF SO3228 0.10 +2.5 3,366,745 - fliF SO3228 0.11 +1.9 3,366,745 - fliF SO3228 0.11 +1.7 3,366,745 - fliF SO3228 0.11 +1.9 3,366,764 - fliF SO3228 0.12 +1.8 3,366,849 - fliF SO3228 0.17 +1.8 3,366,988 + fliF SO3228 0.25 +2.0 3,367,003 - fliF SO3228 0.26 -1.0 3,367,030 - fliF SO3228 0.28 +1.9 3,367,081 - fliF SO3228 0.31 +1.4 3,367,123 - fliF SO3228 0.33 +1.6 3,367,123 - fliF SO3228 0.33 +1.9 3,367,186 - fliF SO3228 0.37 +1.7 3,367,622 + fliF SO3228 0.63 +1.5 3,367,629 + fliF SO3228 0.64 +3.2 3,367,637 - fliF SO3228 0.64 +1.5 3,367,682 - fliF SO3228 0.67 +2.1 3,367,712 + fliF SO3228 0.69 +2.1 3,367,846 - fliF SO3228 0.77 +1.0 3,367,856 - fliF SO3228 0.77 +1.5 3,367,879 - fliF SO3228 0.79 +1.6 3,367,923 + fliF SO3228 0.81 +1.4 3,367,931 - fliF SO3228 0.82 +1.3 3,367,944 - fliF SO3228 0.83 +1.0 3,368,039 - fliF SO3228 0.88 +1.3 3,368,081 + +0.2 3,368,207 - +2.1 3,368,479 + fliE SO3229 0.52 +2.8 3,368,487 - fliE SO3229 0.54 +1.5 3,368,651 + +2.3 3,368,952 - +0.4 3,368,979 + flrC SO3230 0.11 +0.8 3,368,979 + flrC SO3230 0.11 -0.7 3,369,028 + flrC SO3230 0.14 +0.1 3,369,083 - flrC SO3230 0.19 -0.1 3,369,093 - flrC SO3230 0.19 +0.3 3,369,123 - flrC SO3230 0.22 +0.9 3,369,128 - flrC SO3230 0.22 +0.6 3,369,155 - flrC SO3230 0.24 +0.7
Or see this region's nucleotide sequence