Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmU and SO2634 are separated by 3 nucleotides SO2634 and purB are separated by 29 nucleotides purB and SO2636 are separated by 126 nucleotides
SO2633: trmU - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (NCBI ptt file), at 2,768,717 to 2,769,835
trmU
SO2634: SO2634 - conserved hypothetical protein (NCBI ptt file), at 2,769,839 to 2,770,456
SO2634
SO2635: purB - adenylosuccinate lyase (NCBI ptt file), at 2,770,486 to 2,771,856
purB
SO2636: SO2636 - conserved hypothetical protein (NCBI ptt file), at 2,771,983 to 2,773,125
SO2636
Position (kb)
2770
2771
2772 Strain fitness (log2 ratio)
-1
0
1 at 2770.021 kb on + strand, within SO2634 at 2770.364 kb on + strand, within SO2634 at 2770.371 kb on + strand, within SO2634 at 2770.421 kb on + strand at 2771.948 kb on - strand at 2772.370 kb on - strand, within SO2636 at 2772.412 kb on + strand, within SO2636 at 2772.430 kb on - strand, within SO2636 at 2772.727 kb on - strand, within SO2636 at 2772.777 kb on + strand, within SO2636
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,770,021 + SO2634 0.29 +0.3 2,770,364 + SO2634 0.85 +1.0 2,770,371 + SO2634 0.86 -0.2 2,770,421 + -1.3 2,771,948 - -0.4 2,772,370 - SO2636 0.34 -0.4 2,772,412 + SO2636 0.38 +0.6 2,772,430 - SO2636 0.39 +0.6 2,772,727 - SO2636 0.65 -0.0 2,772,777 + SO2636 0.69 +0.6
Or see this region's nucleotide sequence