Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2187 and SO_2188 are separated by 39 nucleotides SO_2188 and SO2189 are separated by 319 nucleotides SO2189 and creA are separated by 17 nucleotides creA and metC are separated by 107 nucleotides
SO2187: SO2187 - ISSod11, transposase (NCBI ptt file), at 2,298,229 to 2,299,185
SO2187
SO_2188: SO_2188 - no description, at 2,299,225 to 2,299,716
_2188
SO2189: SO2189 - hypothetical heat shock protein (NCBI ptt file), at 2,300,036 to 2,301,490
SO2189
SO2190: creA - creA protein (NCBI ptt file), at 2,301,508 to 2,302,005
creA
SO2191: metC - cystathionine beta-lyase (NCBI ptt file), at 2,302,113 to 2,303,312
metC
Position (kb)
2300
2301
2302 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2299.716 kb on + strand at 2299.726 kb on + strand at 2299.758 kb on - strand at 2299.855 kb on + strand at 2299.984 kb on + strand at 2299.984 kb on + strand at 2299.992 kb on - strand at 2300.012 kb on + strand at 2300.082 kb on - strand at 2300.145 kb on - strand at 2300.159 kb on - strand at 2300.339 kb on - strand, within SO2189 at 2300.393 kb on + strand, within SO2189 at 2300.401 kb on - strand, within SO2189 at 2300.419 kb on + strand, within SO2189 at 2300.459 kb on - strand, within SO2189 at 2300.548 kb on - strand, within SO2189 at 2300.594 kb on + strand, within SO2189 at 2300.653 kb on + strand, within SO2189 at 2300.796 kb on + strand, within SO2189 at 2300.796 kb on + strand, within SO2189 at 2300.804 kb on - strand, within SO2189 at 2300.804 kb on - strand, within SO2189 at 2300.804 kb on - strand, within SO2189 at 2300.831 kb on + strand, within SO2189 at 2300.909 kb on + strand, within SO2189 at 2301.064 kb on + strand, within SO2189 at 2301.224 kb on + strand, within SO2189 at 2301.592 kb on + strand, within creA at 2302.069 kb on + strand at 2302.081 kb on - strand at 2302.103 kb on + strand at 2302.229 kb on + strand at 2302.386 kb on - strand, within metC at 2302.463 kb on + strand, within metC
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,299,716 + -1.1 2,299,726 + +0.7 2,299,758 - +0.4 2,299,855 + -1.4 2,299,984 + -0.2 2,299,984 + -1.7 2,299,992 - +0.5 2,300,012 + +1.7 2,300,082 - +0.8 2,300,145 - -0.0 2,300,159 - -0.6 2,300,339 - SO2189 0.21 -0.1 2,300,393 + SO2189 0.25 +0.5 2,300,401 - SO2189 0.25 +0.4 2,300,419 + SO2189 0.26 +2.1 2,300,459 - SO2189 0.29 +1.3 2,300,548 - SO2189 0.35 +0.4 2,300,594 + SO2189 0.38 -0.0 2,300,653 + SO2189 0.42 -0.2 2,300,796 + SO2189 0.52 +3.3 2,300,796 + SO2189 0.52 +0.4 2,300,804 - SO2189 0.53 +0.4 2,300,804 - SO2189 0.53 -0.5 2,300,804 - SO2189 0.53 +0.2 2,300,831 + SO2189 0.55 +0.3 2,300,909 + SO2189 0.60 +0.1 2,301,064 + SO2189 0.71 -0.0 2,301,224 + SO2189 0.82 +0.5 2,301,592 + creA SO2190 0.17 -0.5 2,302,069 + -0.1 2,302,081 - +0.1 2,302,103 + +0.2 2,302,229 + -2.9 2,302,386 - metC SO2191 0.23 -2.2 2,302,463 + metC SO2191 0.29 -2.4
Or see this region's nucleotide sequence