Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pomA and pomB are separated by 3 nucleotides pomB and thiI are separated by 199 nucleotides
SO1529: pomA - chemotaxis motA protein (NCBI ptt file), at 1,604,511 to 1,605,278
pomA
SO1530: pomB - sodium-driven polar flagellar protein PomB (NCBI ptt file), at 1,605,282 to 1,606,208
pomB
SO1531: thiI - thiamine biosynthesis protein ThiI (NCBI ptt file), at 1,606,408 to 1,607,862
thiI
Position (kb)
1605
1606
1607 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1604.347 kb on - strand at 1604.417 kb on + strand at 1604.419 kb on - strand at 1604.431 kb on + strand at 1604.431 kb on + strand at 1604.439 kb on - strand at 1604.482 kb on - strand at 1604.553 kb on - strand at 1604.573 kb on + strand at 1604.735 kb on - strand, within pomA at 1604.901 kb on - strand, within pomA at 1605.281 kb on - strand at 1605.320 kb on - strand at 1605.406 kb on + strand, within pomB at 1605.414 kb on - strand, within pomB at 1605.569 kb on + strand, within pomB at 1605.579 kb on + strand, within pomB at 1605.614 kb on + strand, within pomB at 1605.622 kb on - strand, within pomB at 1605.933 kb on - strand, within pomB at 1606.045 kb on - strand, within pomB at 1606.233 kb on - strand at 1606.293 kb on + strand at 1606.293 kb on + strand at 1606.301 kb on - strand at 1606.321 kb on - strand at 1606.457 kb on - strand at 1606.457 kb on - strand at 1606.496 kb on + strand at 1606.575 kb on + strand, within thiI at 1606.596 kb on + strand, within thiI at 1606.642 kb on + strand, within thiI at 1606.642 kb on + strand, within thiI at 1606.651 kb on + strand, within thiI at 1606.798 kb on - strand, within thiI at 1606.813 kb on - strand, within thiI at 1606.837 kb on + strand, within thiI at 1606.898 kb on - strand, within thiI at 1606.915 kb on + strand, within thiI at 1606.980 kb on - strand, within thiI at 1606.984 kb on + strand, within thiI at 1607.193 kb on - strand, within thiI
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,604,347 - -0.6 1,604,417 + +0.8 1,604,419 - +1.2 1,604,431 + -0.3 1,604,431 + -1.5 1,604,439 - -0.7 1,604,482 - -1.6 1,604,553 - +2.8 1,604,573 + +2.0 1,604,735 - pomA SO1529 0.29 +1.2 1,604,901 - pomA SO1529 0.51 +0.7 1,605,281 - +1.8 1,605,320 - +0.5 1,605,406 + pomB SO1530 0.13 +3.3 1,605,414 - pomB SO1530 0.14 +2.1 1,605,569 + pomB SO1530 0.31 +1.3 1,605,579 + pomB SO1530 0.32 +2.6 1,605,614 + pomB SO1530 0.36 +1.9 1,605,622 - pomB SO1530 0.37 +2.1 1,605,933 - pomB SO1530 0.70 +2.4 1,606,045 - pomB SO1530 0.82 +0.4 1,606,233 - +0.9 1,606,293 + +0.1 1,606,293 + +0.3 1,606,301 - +0.4 1,606,321 - -1.4 1,606,457 - -1.8 1,606,457 - -4.3 1,606,496 + -1.2 1,606,575 + thiI SO1531 0.11 -1.2 1,606,596 + thiI SO1531 0.13 -3.5 1,606,642 + thiI SO1531 0.16 -4.5 1,606,642 + thiI SO1531 0.16 -3.9 1,606,651 + thiI SO1531 0.17 -2.9 1,606,798 - thiI SO1531 0.27 -2.8 1,606,813 - thiI SO1531 0.28 -2.8 1,606,837 + thiI SO1531 0.29 -5.0 1,606,898 - thiI SO1531 0.34 -3.3 1,606,915 + thiI SO1531 0.35 -2.0 1,606,980 - thiI SO1531 0.39 -4.2 1,606,984 + thiI SO1531 0.40 -3.5 1,607,193 - thiI SO1531 0.54 -0.9
Or see this region's nucleotide sequence