Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hflK and hflC are separated by 3 nucleotides hflC and petA are separated by 360 nucleotides petA and petB overlap by 1 nucleotides
SO0605: hflK - hflK protein (NCBI ptt file), at 631,396 to 632,541
hflK
SO0606: hflC - hflC protein (NCBI ptt file), at 632,545 to 633,438
hflC
SO0608: petA - ubiquinol-cytochrome c reductase, iron-sulfur subunit (NCBI ptt file), at 633,799 to 634,389
petA
SO0609: petB - ubiquinol-cytochrome c reductase, cytochrome b (NCBI ptt file), at 634,389 to 635,603
petB
Position (kb) 632
633
634 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 631.550 kb on + strand, within hflK at 631.550 kb on + strand, within hflK at 632.028 kb on + strand, within hflK at 632.119 kb on + strand, within hflK at 632.247 kb on + strand, within hflK at 632.254 kb on + strand, within hflK at 632.259 kb on + strand, within hflK at 632.316 kb on + strand, within hflK at 632.406 kb on + strand, within hflK at 632.430 kb on + strand at 632.430 kb on + strand at 632.430 kb on + strand at 632.590 kb on + strand at 632.744 kb on - strand, within hflC at 632.765 kb on + strand, within hflC at 632.903 kb on + strand, within hflC at 633.052 kb on + strand, within hflC at 633.231 kb on + strand, within hflC at 633.332 kb on + strand, within hflC at 633.845 kb on - strand at 634.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 631,550 + hflK SO0605 0.13 +3.4 631,550 + hflK SO0605 0.13 +4.1 632,028 + hflK SO0605 0.55 +3.9 632,119 + hflK SO0605 0.63 +4.0 632,247 + hflK SO0605 0.74 +4.5 632,254 + hflK SO0605 0.75 +3.2 632,259 + hflK SO0605 0.75 +3.5 632,316 + hflK SO0605 0.80 +4.8 632,406 + hflK SO0605 0.88 +3.9 632,430 + +3.5 632,430 + +4.3 632,430 + +3.4 632,590 + +3.9 632,744 - hflC SO0606 0.22 +3.9 632,765 + hflC SO0606 0.25 +3.3 632,903 + hflC SO0606 0.40 +4.5 633,052 + hflC SO0606 0.57 +4.3 633,231 + hflC SO0606 0.77 +1.5 633,332 + hflC SO0606 0.88 +3.0 633,845 - +2.6 634,379 - +0.4
Or see this region's nucleotide sequence