Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0332 and dsbA are separated by 126 nucleotides dsbA and SO0334 are separated by 448 nucleotides SO0334 and SO0335 overlap by 4 nucleotides
SO0332: SO0332 - hypothetical kinase (NCBI ptt file), at 334,521 to 335,558
SO0332
SO0333: dsbA - thiol:disulfide interchange protein DsbA (NCBI ptt file), at 335,685 to 336,293
dsbA
SO0334: SO0334 - conserved hypothetical protein (NCBI ptt file), at 336,742 to 336,954
SO0334
SO0335: SO0335 - conserved hypothetical protein (NCBI ptt file), at 336,951 to 337,172
SO0335
Position (kb) 335
336
337 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 334.713 kb on + strand, within SO0332 at 334.713 kb on + strand, within SO0332 at 334.834 kb on - strand, within SO0332 at 334.836 kb on + strand, within SO0332 at 334.836 kb on + strand, within SO0332 at 334.836 kb on + strand, within SO0332 at 334.836 kb on + strand, within SO0332 at 334.836 kb on + strand, within SO0332 at 334.836 kb on + strand, within SO0332 at 334.842 kb on + strand, within SO0332 at 334.843 kb on + strand, within SO0332 at 334.844 kb on - strand, within SO0332 at 334.844 kb on - strand, within SO0332 at 334.851 kb on - strand, within SO0332 at 334.851 kb on - strand, within SO0332 at 334.929 kb on - strand, within SO0332 at 334.967 kb on + strand, within SO0332 at 335.004 kb on - strand, within SO0332 at 335.044 kb on + strand, within SO0332 at 335.044 kb on + strand, within SO0332 at 335.062 kb on - strand, within SO0332 at 335.062 kb on - strand, within SO0332 at 335.109 kb on + strand, within SO0332 at 335.120 kb on + strand, within SO0332 at 335.128 kb on - strand, within SO0332 at 335.128 kb on - strand, within SO0332 at 335.128 kb on - strand, within SO0332 at 335.128 kb on - strand, within SO0332 at 335.146 kb on + strand, within SO0332 at 335.369 kb on + strand, within SO0332 at 335.369 kb on + strand, within SO0332 at 335.369 kb on + strand, within SO0332 at 335.377 kb on - strand, within SO0332 at 335.377 kb on - strand, within SO0332 at 335.405 kb on - strand, within SO0332 at 335.425 kb on - strand, within SO0332 at 335.525 kb on + strand at 335.542 kb on + strand at 335.609 kb on - strand at 335.891 kb on + strand, within dsbA at 335.979 kb on + strand, within dsbA at 336.083 kb on + strand, within dsbA at 336.091 kb on + strand, within dsbA at 336.107 kb on - strand, within dsbA at 336.189 kb on + strand, within dsbA at 336.245 kb on + strand at 336.248 kb on - strand at 336.270 kb on + strand at 336.278 kb on - strand at 336.483 kb on + strand at 336.488 kb on + strand at 336.496 kb on - strand at 336.509 kb on - strand at 336.516 kb on - strand at 336.516 kb on - strand at 336.716 kb on - strand at 336.772 kb on - strand, within SO0334 at 336.772 kb on - strand, within SO0334 at 336.772 kb on - strand, within SO0334 at 336.813 kb on - strand, within SO0334 at 336.894 kb on + strand, within SO0334 at 336.907 kb on - strand, within SO0334 at 336.939 kb on + strand at 336.939 kb on + strand at 336.980 kb on - strand, within SO0335 at 337.068 kb on - strand, within SO0335 at 337.170 kb on + strand at 337.170 kb on + strand at 337.170 kb on + strand at 337.170 kb on + strand at 337.170 kb on + strand at 337.170 kb on + strand at 337.170 kb on + strand at 337.170 kb on + strand at 337.178 kb on - strand at 337.178 kb on - strand at 337.178 kb on - strand at 337.178 kb on - strand at 337.178 kb on - strand at 337.180 kb on + strand at 337.180 kb on + strand at 337.180 kb on + strand at 337.180 kb on + strand at 337.180 kb on + strand at 337.180 kb on + strand at 337.188 kb on - strand at 337.188 kb on - strand at 337.188 kb on - strand at 337.188 kb on - strand at 337.188 kb on - strand at 337.188 kb on - strand at 337.188 kb on - strand at 337.188 kb on - strand at 337.240 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 334,713 + SO0332 0.18 -0.1 334,713 + SO0332 0.18 +0.2 334,834 - SO0332 0.30 +0.4 334,836 + SO0332 0.30 -0.6 334,836 + SO0332 0.30 -1.8 334,836 + SO0332 0.30 +1.0 334,836 + SO0332 0.30 -0.3 334,836 + SO0332 0.30 -0.6 334,836 + SO0332 0.30 -2.1 334,842 + SO0332 0.31 -0.8 334,843 + SO0332 0.31 +0.7 334,844 - SO0332 0.31 -1.0 334,844 - SO0332 0.31 -0.1 334,851 - SO0332 0.32 -0.6 334,851 - SO0332 0.32 -1.0 334,929 - SO0332 0.39 -0.4 334,967 + SO0332 0.43 -1.0 335,004 - SO0332 0.47 -0.6 335,044 + SO0332 0.50 -0.8 335,044 + SO0332 0.50 -1.4 335,062 - SO0332 0.52 -1.0 335,062 - SO0332 0.52 +0.6 335,109 + SO0332 0.57 -0.6 335,120 + SO0332 0.58 +1.0 335,128 - SO0332 0.58 -1.4 335,128 - SO0332 0.58 +0.0 335,128 - SO0332 0.58 +1.5 335,128 - SO0332 0.58 -0.2 335,146 + SO0332 0.60 -2.4 335,369 + SO0332 0.82 -0.3 335,369 + SO0332 0.82 +0.0 335,369 + SO0332 0.82 -1.6 335,377 - SO0332 0.82 -0.6 335,377 - SO0332 0.82 -0.5 335,405 - SO0332 0.85 +0.7 335,425 - SO0332 0.87 -0.4 335,525 + +1.6 335,542 + +0.4 335,609 - +2.6 335,891 + dsbA SO0333 0.34 +0.8 335,979 + dsbA SO0333 0.48 +2.9 336,083 + dsbA SO0333 0.65 +1.1 336,091 + dsbA SO0333 0.67 +1.1 336,107 - dsbA SO0333 0.69 -0.1 336,189 + dsbA SO0333 0.83 +0.4 336,245 + +1.2 336,248 - -0.5 336,270 + +0.5 336,278 - -0.1 336,483 + +1.9 336,488 + +0.9 336,496 - -0.1 336,509 - +1.5 336,516 - +1.7 336,516 - +1.0 336,716 - -1.3 336,772 - SO0334 0.14 -0.7 336,772 - SO0334 0.14 -1.4 336,772 - SO0334 0.14 -0.2 336,813 - SO0334 0.33 -0.6 336,894 + SO0334 0.71 -0.5 336,907 - SO0334 0.77 -0.1 336,939 + +0.4 336,939 + +0.0 336,980 - SO0335 0.13 +0.3 337,068 - SO0335 0.53 -0.0 337,170 + +0.6 337,170 + -0.3 337,170 + +1.1 337,170 + +1.0 337,170 + +0.5 337,170 + +1.2 337,170 + +1.5 337,170 + -1.2 337,178 - +0.7 337,178 - -0.3 337,178 - +1.2 337,178 - -0.7 337,178 - +0.7 337,180 + +0.5 337,180 + +0.3 337,180 + +1.2 337,180 + +3.8 337,180 + +0.0 337,180 + +0.6 337,188 - +1.4 337,188 - +0.2 337,188 - -1.7 337,188 - -0.5 337,188 - +0.0 337,188 - -0.8 337,188 - +0.7 337,188 - -0.2 337,240 - +1.5
Or see this region's nucleotide sequence