Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0416 and SO0417 are separated by 73 nucleotides SO0417 and SO0418 are separated by 106 nucleotides SO0418 and SO_0419 are separated by 275 nucleotides SO_0419 and SO0420.2 are separated by 44 nucleotides SO0420.2 and SO0420 are separated by 254 nucleotides SO0420 and ampD overlap by 54 nucleotides
SO0416: SO0416 - type IV pilus biogenesis protein PilB (NCBI ptt file), at 431,608 to 433,317
SO0416
SO0417: SO0417 - pilin, putative (NCBI ptt file), at 433,391 to 433,807
SO0417
SO0418: SO0418 - hypothetical protein (NCBI ptt file), at 433,914 to 434,012
SO0418
SO_0419: SO_0419 - no description, at 434,288 to 434,911
_0419
SO0420.2: SO0420.2 - hypothetical nicotinate-nucleotide pyrophosphorylase (NCBI ptt file), at 434,956 to 435,168
SO0420.2
SO0420: SO0420 - hypothetical protein (NCBI ptt file), at 435,423 to 435,515
SO0420
SO0421: ampD - AmpD protein (NCBI ptt file), at 435,462 to 436,007
ampD
Position (kb) 434
435 Strain fitness (log2 ratio) -2
-1
0
1
2 at 433.291 kb on + strand at 433.291 kb on + strand at 433.296 kb on + strand at 433.305 kb on - strand at 433.343 kb on + strand at 433.361 kb on - strand at 433.361 kb on - strand at 433.437 kb on - strand, within SO0417 at 433.444 kb on - strand, within SO0417 at 433.450 kb on + strand, within SO0417 at 433.455 kb on + strand, within SO0417 at 433.525 kb on - strand, within SO0417 at 433.546 kb on + strand, within SO0417 at 433.546 kb on + strand, within SO0417 at 433.554 kb on - strand, within SO0417 at 433.604 kb on + strand, within SO0417 at 433.634 kb on - strand at 433.815 kb on + strand at 433.815 kb on + strand at 433.815 kb on + strand at 433.815 kb on + strand at 433.817 kb on + strand at 433.823 kb on - strand at 433.825 kb on - strand at 434.106 kb on + strand at 434.106 kb on + strand at 434.136 kb on + strand at 434.169 kb on + strand at 434.311 kb on + strand at 434.320 kb on - strand at 434.320 kb on - strand at 434.328 kb on + strand at 434.380 kb on + strand, within SO_0419 at 434.388 kb on - strand, within SO_0419 at 434.420 kb on - strand, within SO_0419 at 434.728 kb on + strand, within SO_0419 at 434.731 kb on - strand, within SO_0419 at 434.939 kb on - strand at 434.974 kb on + strand at 434.987 kb on - strand, within SO0420.2 at 435.235 kb on - strand at 435.256 kb on - strand at 435.266 kb on - strand at 435.278 kb on + strand at 435.278 kb on + strand at 435.278 kb on + strand at 435.286 kb on - strand at 435.393 kb on + strand at 435.431 kb on - strand at 435.431 kb on - strand at 435.514 kb on + strand at 435.544 kb on + strand, within ampD at 435.546 kb on - strand, within ampD at 435.591 kb on - strand, within ampD at 435.601 kb on - strand, within ampD at 435.601 kb on - strand, within ampD at 435.601 kb on - strand, within ampD at 435.646 kb on + strand, within ampD at 435.664 kb on + strand, within ampD at 435.724 kb on - strand, within ampD at 435.731 kb on + strand, within ampD at 435.744 kb on - strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.835 kb on - strand, within ampD at 435.835 kb on - strand, within ampD at 435.897 kb on - strand, within ampD at 435.897 kb on - strand, within ampD
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 433,291 + -0.5 433,291 + -0.2 433,296 + +1.2 433,305 - +0.9 433,343 + -0.1 433,361 - -0.4 433,361 - +0.7 433,437 - SO0417 0.11 +0.7 433,444 - SO0417 0.13 +0.1 433,450 + SO0417 0.14 +1.5 433,455 + SO0417 0.15 +1.8 433,525 - SO0417 0.32 +0.7 433,546 + SO0417 0.37 -0.3 433,546 + SO0417 0.37 +0.4 433,554 - SO0417 0.39 +1.1 433,604 + SO0417 0.51 +2.4 433,634 - +0.3 433,815 + +0.5 433,815 + +1.0 433,815 + +0.7 433,815 + +0.4 433,817 + +1.5 433,823 - -0.5 433,825 - +0.5 434,106 + +0.8 434,106 + +0.9 434,136 + +0.9 434,169 + +1.5 434,311 + -2.5 434,320 - -0.2 434,320 - -1.3 434,328 + -1.9 434,380 + SO_0419 0.15 -2.5 434,388 - SO_0419 0.16 -1.3 434,420 - SO_0419 0.21 -1.8 434,728 + SO_0419 0.71 -2.5 434,731 - SO_0419 0.71 -1.8 434,939 - +0.5 434,974 + -2.2 434,987 - SO0420.2 0.15 -2.2 435,235 - -0.3 435,256 - +0.4 435,266 - -0.7 435,278 + -0.1 435,278 + +0.1 435,278 + +0.0 435,286 - +1.2 435,393 + -0.5 435,431 - -1.5 435,431 - -0.5 435,514 + +0.4 435,544 + ampD SO0421 0.15 -0.7 435,546 - ampD SO0421 0.15 +0.6 435,591 - ampD SO0421 0.24 +1.2 435,601 - ampD SO0421 0.25 -0.5 435,601 - ampD SO0421 0.25 -0.5 435,601 - ampD SO0421 0.25 -0.6 435,646 + ampD SO0421 0.34 -0.7 435,664 + ampD SO0421 0.37 -0.0 435,724 - ampD SO0421 0.48 -0.7 435,731 + ampD SO0421 0.49 +0.3 435,744 - ampD SO0421 0.52 +0.5 435,827 + ampD SO0421 0.67 -0.9 435,827 + ampD SO0421 0.67 -0.6 435,827 + ampD SO0421 0.67 -0.0 435,827 + ampD SO0421 0.67 -1.0 435,827 + ampD SO0421 0.67 +1.2 435,827 + ampD SO0421 0.67 +2.4 435,827 + ampD SO0421 0.67 +0.2 435,835 - ampD SO0421 0.68 +0.2 435,835 - ampD SO0421 0.68 +0.4 435,897 - ampD SO0421 0.80 -2.2 435,897 - ampD SO0421 0.80 +0.3
Or see this region's nucleotide sequence