Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00980 and PGA1_c00990 are separated by 139 nucleotides PGA1_c00990 and PGA1_c01000 are separated by 92 nucleotides PGA1_c01000 and PGA1_c01010 are separated by 40 nucleotides
PGA1_c00980: PGA1_c00980 - Uncharacterized conserved protein, at 92,997 to 93,566
_c00980
PGA1_c00990: PGA1_c00990 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase FolK, at 93,706 to 94,275
_c00990
PGA1_c01000: PGA1_c01000 - DNA-directed RNA polymerase subunit omega, at 94,368 to 94,721
_c01000
PGA1_c01010: PGA1_c01010 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase SpoT, at 94,762 to 96,894
_c01010
Position (kb) 94
95 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 93.413 kb on + strand, within PGA1_c00980 at 93.418 kb on + strand, within PGA1_c00980 at 93.421 kb on - strand, within PGA1_c00980 at 93.421 kb on - strand, within PGA1_c00980 at 93.422 kb on + strand, within PGA1_c00980 at 93.426 kb on - strand, within PGA1_c00980 at 93.430 kb on - strand, within PGA1_c00980 at 93.439 kb on + strand, within PGA1_c00980 at 93.466 kb on - strand, within PGA1_c00980 at 93.494 kb on - strand, within PGA1_c00980 at 93.511 kb on + strand at 93.511 kb on + strand at 93.511 kb on + strand at 93.511 kb on + strand at 93.511 kb on + strand at 93.518 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.539 kb on + strand at 93.547 kb on + strand at 93.547 kb on - strand at 93.547 kb on - strand at 93.590 kb on + strand at 93.597 kb on + strand at 93.598 kb on - strand at 93.608 kb on - strand at 93.643 kb on + strand at 93.652 kb on - strand at 94.269 kb on + strand at 94.269 kb on + strand at 94.269 kb on + strand at 94.277 kb on - strand at 94.277 kb on - strand at 94.300 kb on - strand at 94.632 kb on - strand, within PGA1_c01000 at 94.646 kb on + strand, within PGA1_c01000 at 94.718 kb on - strand at 94.723 kb on - strand at 94.786 kb on + strand at 94.794 kb on - strand at 94.794 kb on - strand at 94.815 kb on - strand at 94.815 kb on - strand at 94.889 kb on + strand at 94.893 kb on - strand at 94.903 kb on - strand at 94.940 kb on + strand at 94.964 kb on + strand at 95.023 kb on + strand, within PGA1_c01010 at 95.033 kb on - strand, within PGA1_c01010 at 95.033 kb on - strand, within PGA1_c01010 at 95.056 kb on + strand, within PGA1_c01010 at 95.127 kb on + strand, within PGA1_c01010 at 95.127 kb on + strand, within PGA1_c01010 at 95.135 kb on - strand, within PGA1_c01010 at 95.135 kb on - strand, within PGA1_c01010 at 95.147 kb on - strand, within PGA1_c01010 at 95.171 kb on - strand, within PGA1_c01010 at 95.178 kb on - strand, within PGA1_c01010 at 95.280 kb on - strand, within PGA1_c01010 at 95.328 kb on + strand, within PGA1_c01010 at 95.333 kb on + strand, within PGA1_c01010 at 95.333 kb on + strand, within PGA1_c01010 at 95.336 kb on - strand, within PGA1_c01010 at 95.341 kb on - strand, within PGA1_c01010 at 95.439 kb on - strand, within PGA1_c01010 at 95.520 kb on - strand, within PGA1_c01010 at 95.520 kb on - strand, within PGA1_c01010 at 95.579 kb on - strand, within PGA1_c01010 at 95.583 kb on - strand, within PGA1_c01010 at 95.612 kb on + strand, within PGA1_c01010 at 95.620 kb on - strand, within PGA1_c01010 at 95.634 kb on - strand, within PGA1_c01010 at 95.642 kb on + strand, within PGA1_c01010 at 95.650 kb on - strand, within PGA1_c01010 at 95.719 kb on - strand, within PGA1_c01010
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 93,413 + PGA1_c00980 0.73 -1.4 93,418 + PGA1_c00980 0.74 -2.4 93,421 - PGA1_c00980 0.74 +0.1 93,421 - PGA1_c00980 0.74 -1.5 93,422 + PGA1_c00980 0.75 -1.4 93,426 - PGA1_c00980 0.75 -1.7 93,430 - PGA1_c00980 0.76 +2.0 93,439 + PGA1_c00980 0.78 -2.3 93,466 - PGA1_c00980 0.82 -0.2 93,494 - PGA1_c00980 0.87 -1.0 93,511 + -0.3 93,511 + -4.0 93,511 + -0.7 93,511 + -3.7 93,511 + -1.9 93,518 + -0.8 93,529 + -3.2 93,529 + -2.5 93,539 + -1.7 93,547 + -1.9 93,547 - -1.1 93,547 - -1.9 93,590 + -0.5 93,597 + -0.5 93,598 - -0.1 93,608 - -1.0 93,643 + -0.6 93,652 - -1.9 94,269 + -0.3 94,269 + -0.1 94,269 + -2.0 94,277 - -1.4 94,277 - +0.9 94,300 - +0.4 94,632 - PGA1_c01000 0.75 -1.9 94,646 + PGA1_c01000 0.79 -3.7 94,718 - +0.6 94,723 - -1.0 94,786 + -3.8 94,794 - -3.2 94,794 - -3.2 94,815 - -2.3 94,815 - -2.0 94,889 + -3.4 94,893 - -3.5 94,903 - -4.1 94,940 + -2.5 94,964 + -2.4 95,023 + PGA1_c01010 0.12 -2.8 95,033 - PGA1_c01010 0.13 -2.5 95,033 - PGA1_c01010 0.13 -5.5 95,056 + PGA1_c01010 0.14 -2.3 95,127 + PGA1_c01010 0.17 -1.7 95,127 + PGA1_c01010 0.17 -2.6 95,135 - PGA1_c01010 0.17 -2.7 95,135 - PGA1_c01010 0.17 -2.7 95,147 - PGA1_c01010 0.18 -2.2 95,171 - PGA1_c01010 0.19 -3.7 95,178 - PGA1_c01010 0.20 -4.1 95,280 - PGA1_c01010 0.24 -2.3 95,328 + PGA1_c01010 0.27 -4.8 95,333 + PGA1_c01010 0.27 -3.2 95,333 + PGA1_c01010 0.27 -3.8 95,336 - PGA1_c01010 0.27 -3.8 95,341 - PGA1_c01010 0.27 -1.8 95,439 - PGA1_c01010 0.32 -3.9 95,520 - PGA1_c01010 0.36 -2.8 95,520 - PGA1_c01010 0.36 -3.4 95,579 - PGA1_c01010 0.38 -5.4 95,583 - PGA1_c01010 0.38 -1.0 95,612 + PGA1_c01010 0.40 -2.8 95,620 - PGA1_c01010 0.40 -0.6 95,634 - PGA1_c01010 0.41 -3.6 95,642 + PGA1_c01010 0.41 -3.2 95,650 - PGA1_c01010 0.42 -3.8 95,719 - PGA1_c01010 0.45 -3.7
Or see this region's nucleotide sequence