Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06420 and PGA1_c06430 are separated by 96 nucleotides PGA1_c06430 and PGA1_c06440 are separated by 121 nucleotides PGA1_c06440 and PGA1_c06450 are separated by 13 nucleotides PGA1_c06450 and PGA1_c06460 are separated by 118 nucleotides
PGA1_c06420: PGA1_c06420 - acetyltransferase domain-containing protein, at 657,419 to 657,928
_c06420
PGA1_c06430: PGA1_c06430 - hypothetical protein, at 658,025 to 658,804
_c06430
PGA1_c06440: PGA1_c06440 - Uncharacterized protein conserved in bacteria, at 658,926 to 659,456
_c06440
PGA1_c06450: PGA1_c06450 - short-chain dehydrogenases/reductase domain-containing protein, at 659,470 to 660,111
_c06450
PGA1_c06460: PGA1_c06460 - Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain, at 660,230 to 662,008
_c06460
Position (kb)
658
659
660 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 658.052 kb on + strand at 658.052 kb on + strand at 658.057 kb on + strand at 658.060 kb on - strand at 658.065 kb on - strand at 658.065 kb on - strand at 658.065 kb on - strand at 658.229 kb on - strand, within PGA1_c06430 at 658.459 kb on + strand, within PGA1_c06430 at 658.467 kb on + strand, within PGA1_c06430 at 658.475 kb on - strand, within PGA1_c06430 at 658.549 kb on + strand, within PGA1_c06430 at 658.562 kb on + strand, within PGA1_c06430 at 658.570 kb on - strand, within PGA1_c06430 at 658.599 kb on + strand, within PGA1_c06430 at 658.654 kb on + strand, within PGA1_c06430 at 658.721 kb on - strand, within PGA1_c06430 at 658.721 kb on - strand, within PGA1_c06430 at 658.786 kb on - strand at 658.825 kb on - strand at 658.835 kb on + strand at 658.840 kb on + strand at 658.876 kb on + strand at 658.884 kb on - strand at 658.907 kb on + strand at 658.907 kb on + strand at 658.915 kb on + strand at 658.915 kb on + strand at 659.027 kb on - strand, within PGA1_c06440 at 659.103 kb on - strand, within PGA1_c06440 at 659.239 kb on + strand, within PGA1_c06440 at 659.259 kb on + strand, within PGA1_c06440 at 659.273 kb on + strand, within PGA1_c06440 at 659.274 kb on + strand, within PGA1_c06440 at 659.284 kb on + strand, within PGA1_c06440 at 659.312 kb on - strand, within PGA1_c06440 at 659.369 kb on + strand, within PGA1_c06440 at 659.384 kb on + strand, within PGA1_c06440 at 659.415 kb on + strand at 659.420 kb on + strand at 659.437 kb on - strand at 659.534 kb on - strand at 659.550 kb on + strand, within PGA1_c06450 at 659.566 kb on - strand, within PGA1_c06450 at 659.581 kb on - strand, within PGA1_c06450 at 659.639 kb on - strand, within PGA1_c06450 at 659.711 kb on - strand, within PGA1_c06450 at 659.713 kb on + strand, within PGA1_c06450 at 659.713 kb on + strand, within PGA1_c06450 at 659.721 kb on - strand, within PGA1_c06450 at 659.721 kb on - strand, within PGA1_c06450 at 659.726 kb on - strand, within PGA1_c06450 at 659.726 kb on - strand, within PGA1_c06450 at 659.730 kb on + strand, within PGA1_c06450 at 659.795 kb on - strand, within PGA1_c06450 at 659.798 kb on + strand, within PGA1_c06450 at 659.839 kb on + strand, within PGA1_c06450 at 659.864 kb on + strand, within PGA1_c06450 at 659.870 kb on - strand, within PGA1_c06450 at 659.887 kb on - strand, within PGA1_c06450 at 660.028 kb on + strand, within PGA1_c06450 at 660.059 kb on - strand at 660.059 kb on - strand at 660.092 kb on + strand at 660.141 kb on + strand at 660.155 kb on + strand at 660.187 kb on + strand at 660.223 kb on + strand at 660.269 kb on - strand at 660.271 kb on + strand at 660.283 kb on - strand at 660.327 kb on - strand at 660.331 kb on + strand at 660.331 kb on + strand at 660.331 kb on + strand at 660.331 kb on + strand at 660.339 kb on - strand at 660.349 kb on - strand at 660.349 kb on - strand at 660.349 kb on - strand at 660.360 kb on + strand at 660.387 kb on + strand at 660.387 kb on + strand at 660.387 kb on + strand at 660.431 kb on + strand, within PGA1_c06460
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 658,052 + -0.6 658,052 + -0.1 658,057 + +0.5 658,060 - -0.3 658,065 - +0.5 658,065 - -3.2 658,065 - -0.5 658,229 - PGA1_c06430 0.26 -0.2 658,459 + PGA1_c06430 0.56 -0.1 658,467 + PGA1_c06430 0.57 +1.0 658,475 - PGA1_c06430 0.58 -0.2 658,549 + PGA1_c06430 0.67 -0.5 658,562 + PGA1_c06430 0.69 +0.5 658,570 - PGA1_c06430 0.70 -2.5 658,599 + PGA1_c06430 0.74 +0.6 658,654 + PGA1_c06430 0.81 -0.2 658,721 - PGA1_c06430 0.89 +1.3 658,721 - PGA1_c06430 0.89 -0.7 658,786 - -0.3 658,825 - -0.2 658,835 + -1.1 658,840 + +0.4 658,876 + +0.5 658,884 - +1.3 658,907 + +1.5 658,907 + -0.0 658,915 + +1.6 658,915 + -0.9 659,027 - PGA1_c06440 0.19 -0.7 659,103 - PGA1_c06440 0.33 +0.3 659,239 + PGA1_c06440 0.59 +0.3 659,259 + PGA1_c06440 0.63 +0.0 659,273 + PGA1_c06440 0.65 +0.2 659,274 + PGA1_c06440 0.66 +0.8 659,284 + PGA1_c06440 0.67 -0.0 659,312 - PGA1_c06440 0.73 -1.0 659,369 + PGA1_c06440 0.83 -0.0 659,384 + PGA1_c06440 0.86 -0.5 659,415 + -0.6 659,420 + +0.1 659,437 - -0.2 659,534 - +0.2 659,550 + PGA1_c06450 0.12 +0.2 659,566 - PGA1_c06450 0.15 -1.7 659,581 - PGA1_c06450 0.17 +0.9 659,639 - PGA1_c06450 0.26 +0.1 659,711 - PGA1_c06450 0.38 -0.1 659,713 + PGA1_c06450 0.38 +0.1 659,713 + PGA1_c06450 0.38 +0.7 659,721 - PGA1_c06450 0.39 +0.5 659,721 - PGA1_c06450 0.39 -0.1 659,726 - PGA1_c06450 0.40 -3.0 659,726 - PGA1_c06450 0.40 -0.1 659,730 + PGA1_c06450 0.40 -0.9 659,795 - PGA1_c06450 0.51 +1.2 659,798 + PGA1_c06450 0.51 +0.2 659,839 + PGA1_c06450 0.57 -0.1 659,864 + PGA1_c06450 0.61 +1.5 659,870 - PGA1_c06450 0.62 -1.4 659,887 - PGA1_c06450 0.65 +0.0 660,028 + PGA1_c06450 0.87 -0.1 660,059 - -0.0 660,059 - +0.8 660,092 + +0.1 660,141 + -0.5 660,155 + +0.0 660,187 + -0.7 660,223 + -0.5 660,269 - -1.1 660,271 + +0.3 660,283 - -1.7 660,327 - -1.1 660,331 + -0.5 660,331 + -2.7 660,331 + -1.1 660,331 + -0.9 660,339 - +0.3 660,349 - +0.2 660,349 - +0.5 660,349 - -1.9 660,360 + -0.9 660,387 + -0.6 660,387 + -0.1 660,387 + -0.3 660,431 + PGA1_c06460 0.11 -0.1
Or see this region's nucleotide sequence