Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03730 and PGA1_c03740 are separated by 192 nucleotides PGA1_c03740 and PGA1_c03750 are separated by 18 nucleotides PGA1_c03750 and PGA1_c03760 are separated by 15 nucleotides PGA1_c03760 and PGA1_c03770 are separated by 84 nucleotides
PGA1_c03730: PGA1_c03730 - Protein of unknown function (DUF1194)., at 358,494 to 359,183
_c03730
PGA1_c03740: PGA1_c03740 - succinate dehydrogenase cytochrome b556 subunit, at 359,376 to 359,759
_c03740
PGA1_c03750: PGA1_c03750 - succinate dehydrogenase hydrophobic membrane anchor subunit, at 359,778 to 360,149
_c03750
PGA1_c03760: PGA1_c03760 - succinate dehydrogenase flavoprotein subunit, at 360,165 to 361,970
_c03760
PGA1_c03770: PGA1_c03770 - hypothetical protein, at 362,055 to 362,969
_c03770
Position (kb) 360
361
362 Strain fitness (log2 ratio) -2
-1
0
1 at 359.169 kb on - strand at 359.169 kb on - strand at 359.169 kb on - strand at 359.203 kb on - strand at 359.758 kb on + strand at 360.126 kb on + strand at 361.972 kb on + strand at 362.040 kb on + strand at 362.042 kb on + strand at 362.042 kb on + strand at 362.494 kb on + strand, within PGA1_c03770 at 362.516 kb on + strand, within PGA1_c03770 at 362.516 kb on + strand, within PGA1_c03770 at 362.630 kb on + strand, within PGA1_c03770 at 362.735 kb on + strand, within PGA1_c03770 at 362.737 kb on - strand, within PGA1_c03770 at 362.754 kb on + strand, within PGA1_c03770 at 362.766 kb on + strand, within PGA1_c03770 at 362.766 kb on + strand, within PGA1_c03770 at 362.852 kb on - strand, within PGA1_c03770 at 362.884 kb on - strand at 362.899 kb on + strand at 362.963 kb on + strand at 362.963 kb on + strand at 362.963 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 359,169 - +0.9 359,169 - +0.8 359,169 - +0.4 359,203 - -2.5 359,758 + -0.8 360,126 + +0.9 361,972 + -0.6 362,040 + -2.5 362,042 + -0.5 362,042 + +1.0 362,494 + PGA1_c03770 0.48 -2.0 362,516 + PGA1_c03770 0.50 -2.4 362,516 + PGA1_c03770 0.50 -0.8 362,630 + PGA1_c03770 0.63 +1.1 362,735 + PGA1_c03770 0.74 -0.2 362,737 - PGA1_c03770 0.75 -2.5 362,754 + PGA1_c03770 0.76 +0.0 362,766 + PGA1_c03770 0.78 +0.5 362,766 + PGA1_c03770 0.78 -1.6 362,852 - PGA1_c03770 0.87 -2.4 362,884 - -0.9 362,899 + -0.4 362,963 + +1.0 362,963 + +0.1 362,963 + -2.4
Or see this region's nucleotide sequence