Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0627 and Sama_0628 are separated by 303 nucleotides Sama_0628 and Sama_0629 are separated by 1 nucleotides Sama_0629 and rumB are separated by 34 nucleotides
Sama_0627: Sama_0627 - hypothetical protein (RefSeq), at 780,003 to 780,449
_0627
Sama_0628: Sama_0628 - MarR family transcriptional regulator (RefSeq), at 780,753 to 781,187
_0628
Sama_0629: Sama_0629 - glycerol-3-phosphate dehydrogenase (RefSeq), at 781,189 to 782,403
_0629
Sama_0630: rumB - 23S rRNA methyluridine methyltransferase (RefSeq), at 782,438 to 783,592
rumB
Position (kb) 781
782
783 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 780.200 kb on + strand, within Sama_0627 at 780.200 kb on + strand, within Sama_0627 at 780.200 kb on + strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.201 kb on - strand, within Sama_0627 at 780.206 kb on - strand, within Sama_0627 at 780.206 kb on - strand, within Sama_0627 at 780.206 kb on - strand, within Sama_0627 at 780.262 kb on + strand, within Sama_0627 at 780.263 kb on - strand, within Sama_0627 at 780.263 kb on - strand, within Sama_0627 at 780.263 kb on - strand, within Sama_0627 at 780.269 kb on + strand, within Sama_0627 at 780.270 kb on - strand, within Sama_0627 at 780.270 kb on - strand, within Sama_0627 at 780.270 kb on - strand, within Sama_0627 at 780.270 kb on - strand, within Sama_0627 at 780.270 kb on - strand, within Sama_0627 at 780.340 kb on + strand, within Sama_0627 at 780.341 kb on - strand, within Sama_0627 at 780.341 kb on - strand, within Sama_0627 at 780.389 kb on + strand, within Sama_0627 at 780.431 kb on + strand at 780.432 kb on - strand at 780.432 kb on - strand at 780.432 kb on - strand at 780.432 kb on - strand at 780.435 kb on + strand at 780.435 kb on + strand at 780.436 kb on - strand at 780.437 kb on + strand at 780.437 kb on + strand at 780.437 kb on + strand at 780.439 kb on + strand at 780.439 kb on + strand at 780.439 kb on + strand at 780.439 kb on + strand at 780.440 kb on - strand at 780.440 kb on - strand at 780.508 kb on - strand at 780.537 kb on - strand at 780.550 kb on + strand at 780.550 kb on + strand at 780.551 kb on - strand at 780.639 kb on + strand at 780.640 kb on - strand at 780.640 kb on - strand at 780.705 kb on + strand at 780.706 kb on - strand at 780.716 kb on + strand at 780.716 kb on + strand at 780.716 kb on + strand at 780.717 kb on - strand at 780.731 kb on - strand at 780.732 kb on + strand at 780.732 kb on + strand at 780.732 kb on + strand at 780.761 kb on + strand at 780.761 kb on + strand at 780.762 kb on - strand at 780.762 kb on - strand at 780.799 kb on - strand, within Sama_0628 at 780.828 kb on + strand, within Sama_0628 at 780.828 kb on + strand, within Sama_0628 at 780.838 kb on + strand, within Sama_0628 at 780.861 kb on + strand, within Sama_0628 at 780.861 kb on + strand, within Sama_0628 at 780.861 kb on + strand, within Sama_0628 at 780.861 kb on + strand, within Sama_0628 at 780.862 kb on - strand, within Sama_0628 at 780.862 kb on - strand, within Sama_0628 at 780.870 kb on + strand, within Sama_0628 at 780.871 kb on - strand, within Sama_0628 at 780.871 kb on - strand, within Sama_0628 at 780.871 kb on - strand, within Sama_0628 at 780.871 kb on - strand, within Sama_0628 at 780.871 kb on - strand, within Sama_0628 at 780.871 kb on - strand, within Sama_0628 at 780.955 kb on + strand, within Sama_0628 at 781.083 kb on - strand, within Sama_0628 at 781.185 kb on + strand at 781.185 kb on + strand at 781.185 kb on + strand at 781.185 kb on + strand at 781.185 kb on + strand at 781.185 kb on + strand at 781.186 kb on - strand at 781.186 kb on - strand at 781.186 kb on - strand at 781.186 kb on - strand at 781.186 kb on - strand at 781.186 kb on - strand at 781.242 kb on - strand at 781.268 kb on - strand at 781.351 kb on + strand, within Sama_0629 at 781.351 kb on + strand, within Sama_0629 at 781.352 kb on - strand, within Sama_0629 at 781.352 kb on - strand, within Sama_0629 at 781.362 kb on + strand, within Sama_0629 at 781.362 kb on + strand, within Sama_0629 at 781.362 kb on + strand, within Sama_0629 at 781.362 kb on + strand, within Sama_0629 at 781.362 kb on + strand, within Sama_0629 at 781.362 kb on + strand, within Sama_0629 at 781.363 kb on - strand, within Sama_0629 at 781.363 kb on - strand, within Sama_0629 at 781.363 kb on - strand, within Sama_0629 at 781.363 kb on - strand, within Sama_0629 at 781.363 kb on - strand, within Sama_0629 at 781.383 kb on + strand, within Sama_0629 at 781.383 kb on + strand, within Sama_0629 at 781.383 kb on + strand, within Sama_0629 at 781.383 kb on + strand, within Sama_0629 at 781.384 kb on - strand, within Sama_0629 at 781.384 kb on - strand, within Sama_0629 at 781.384 kb on - strand, within Sama_0629 at 781.384 kb on - strand, within Sama_0629 at 781.384 kb on - strand, within Sama_0629 at 781.384 kb on - strand, within Sama_0629 at 781.384 kb on - strand, within Sama_0629 at 781.412 kb on + strand, within Sama_0629 at 781.412 kb on + strand, within Sama_0629 at 781.412 kb on + strand, within Sama_0629 at 781.413 kb on - strand, within Sama_0629 at 781.413 kb on - strand, within Sama_0629 at 781.450 kb on + strand, within Sama_0629 at 781.450 kb on + strand, within Sama_0629 at 781.462 kb on + strand, within Sama_0629 at 781.463 kb on - strand, within Sama_0629 at 781.463 kb on - strand, within Sama_0629 at 781.463 kb on - strand, within Sama_0629 at 781.492 kb on - strand, within Sama_0629 at 781.513 kb on + strand, within Sama_0629 at 781.514 kb on - strand, within Sama_0629 at 781.523 kb on + strand, within Sama_0629 at 781.523 kb on + strand, within Sama_0629 at 781.524 kb on - strand, within Sama_0629 at 781.592 kb on + strand, within Sama_0629 at 781.592 kb on + strand, within Sama_0629 at 781.593 kb on - strand, within Sama_0629 at 781.624 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.727 kb on + strand, within Sama_0629 at 781.728 kb on - strand, within Sama_0629 at 781.728 kb on - strand, within Sama_0629 at 781.728 kb on - strand, within Sama_0629 at 781.728 kb on - strand, within Sama_0629 at 781.728 kb on - strand, within Sama_0629 at 781.728 kb on - strand, within Sama_0629 at 781.728 kb on - strand, within Sama_0629 at 781.728 kb on - strand, within Sama_0629 at 781.728 kb on - strand, within Sama_0629 at 781.728 kb on - strand, within Sama_0629 at 781.728 kb on - strand, within Sama_0629 at 781.761 kb on + strand, within Sama_0629 at 781.762 kb on - strand, within Sama_0629 at 781.762 kb on - strand, within Sama_0629 at 781.910 kb on + strand, within Sama_0629 at 781.910 kb on + strand, within Sama_0629 at 781.911 kb on - strand, within Sama_0629 at 781.911 kb on - strand, within Sama_0629 at 781.933 kb on + strand, within Sama_0629 at 781.934 kb on - strand, within Sama_0629 at 781.934 kb on - strand, within Sama_0629 at 781.934 kb on - strand, within Sama_0629 at 781.934 kb on - strand, within Sama_0629 at 781.971 kb on + strand, within Sama_0629 at 781.972 kb on - strand, within Sama_0629 at 781.972 kb on - strand, within Sama_0629 at 781.972 kb on - strand, within Sama_0629 at 781.972 kb on - strand, within Sama_0629 at 782.065 kb on + strand, within Sama_0629 at 782.065 kb on + strand, within Sama_0629 at 782.080 kb on + strand, within Sama_0629 at 782.081 kb on - strand, within Sama_0629 at 782.081 kb on - strand, within Sama_0629 at 782.081 kb on - strand, within Sama_0629 at 782.089 kb on + strand, within Sama_0629 at 782.090 kb on - strand, within Sama_0629 at 782.090 kb on - strand, within Sama_0629 at 782.090 kb on - strand, within Sama_0629 at 782.090 kb on - strand, within Sama_0629 at 782.090 kb on - strand, within Sama_0629 at 782.090 kb on - strand, within Sama_0629 at 782.139 kb on + strand, within Sama_0629 at 782.140 kb on - strand, within Sama_0629 at 782.140 kb on - strand, within Sama_0629 at 782.140 kb on - strand, within Sama_0629 at 782.249 kb on - strand, within Sama_0629 at 782.249 kb on - strand, within Sama_0629 at 782.323 kb on - strand at 782.346 kb on + strand at 782.385 kb on + strand at 782.402 kb on - strand at 782.420 kb on + strand at 782.450 kb on + strand at 782.451 kb on - strand at 782.705 kb on + strand, within rumB at 782.764 kb on + strand, within rumB at 782.765 kb on - strand, within rumB at 782.803 kb on + strand, within rumB at 782.803 kb on + strand, within rumB at 782.804 kb on - strand, within rumB at 782.804 kb on - strand, within rumB at 782.804 kb on - strand, within rumB at 782.804 kb on - strand, within rumB at 782.804 kb on - strand, within rumB at 782.804 kb on - strand, within rumB at 782.810 kb on - strand, within rumB at 782.810 kb on - strand, within rumB at 782.845 kb on + strand, within rumB at 782.845 kb on + strand, within rumB at 782.846 kb on - strand, within rumB at 782.846 kb on - strand, within rumB at 782.846 kb on - strand, within rumB at 782.932 kb on + strand, within rumB at 782.932 kb on + strand, within rumB at 782.932 kb on + strand, within rumB at 782.932 kb on + strand, within rumB at 782.933 kb on - strand, within rumB at 783.027 kb on + strand, within rumB at 783.027 kb on + strand, within rumB at 783.027 kb on + strand, within rumB at 783.099 kb on + strand, within rumB at 783.100 kb on - strand, within rumB at 783.102 kb on - strand, within rumB at 783.132 kb on + strand, within rumB at 783.132 kb on + strand, within rumB at 783.132 kb on + strand, within rumB at 783.133 kb on - strand, within rumB at 783.133 kb on - strand, within rumB at 783.133 kb on - strand, within rumB at 783.169 kb on - strand, within rumB at 783.280 kb on + strand, within rumB at 783.357 kb on + strand, within rumB at 783.357 kb on + strand, within rumB at 783.357 kb on + strand, within rumB at 783.357 kb on + strand, within rumB
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 780,200 + Sama_0627 0.44 +0.7 780,200 + Sama_0627 0.44 -1.1 780,200 + Sama_0627 0.44 +0.9 780,201 - Sama_0627 0.44 -1.7 780,201 - Sama_0627 0.44 +1.0 780,201 - Sama_0627 0.44 +0.4 780,201 - Sama_0627 0.44 +0.5 780,201 - Sama_0627 0.44 +0.6 780,201 - Sama_0627 0.44 -0.7 780,201 - Sama_0627 0.44 +0.6 780,201 - Sama_0627 0.44 -0.5 780,201 - Sama_0627 0.44 +0.0 780,201 - Sama_0627 0.44 -2.3 780,201 - Sama_0627 0.44 -0.2 780,201 - Sama_0627 0.44 +0.6 780,206 - Sama_0627 0.45 +0.1 780,206 - Sama_0627 0.45 -1.9 780,206 - Sama_0627 0.45 -0.5 780,262 + Sama_0627 0.58 -0.9 780,263 - Sama_0627 0.58 -1.3 780,263 - Sama_0627 0.58 -0.2 780,263 - Sama_0627 0.58 +0.0 780,269 + Sama_0627 0.60 -0.8 780,270 - Sama_0627 0.60 -0.2 780,270 - Sama_0627 0.60 -1.7 780,270 - Sama_0627 0.60 +0.4 780,270 - Sama_0627 0.60 -0.0 780,270 - Sama_0627 0.60 -0.9 780,340 + Sama_0627 0.75 +0.6 780,341 - Sama_0627 0.76 -2.3 780,341 - Sama_0627 0.76 +0.4 780,389 + Sama_0627 0.86 -0.1 780,431 + -1.1 780,432 - -1.8 780,432 - -0.5 780,432 - +0.2 780,432 - -2.5 780,435 + -2.1 780,435 + -2.1 780,436 - +0.5 780,437 + -0.4 780,437 + +1.3 780,437 + +0.7 780,439 + +0.4 780,439 + -0.0 780,439 + +1.1 780,439 + -0.5 780,440 - -0.0 780,440 - +0.3 780,508 - +2.3 780,537 - +0.4 780,550 + +2.0 780,550 + -0.9 780,551 - -0.7 780,639 + +0.7 780,640 - +1.7 780,640 - +0.7 780,705 + -0.2 780,706 - +1.1 780,716 + -2.0 780,716 + -3.4 780,716 + -2.3 780,717 - +2.2 780,731 - -0.6 780,732 + -0.2 780,732 + +2.0 780,732 + -0.5 780,761 + +2.4 780,761 + +0.8 780,762 - -0.3 780,762 - +2.1 780,799 - Sama_0628 0.11 +1.2 780,828 + Sama_0628 0.17 +1.0 780,828 + Sama_0628 0.17 +2.9 780,838 + Sama_0628 0.20 +2.4 780,861 + Sama_0628 0.25 +3.4 780,861 + Sama_0628 0.25 +0.6 780,861 + Sama_0628 0.25 +2.6 780,861 + Sama_0628 0.25 +2.2 780,862 - Sama_0628 0.25 +1.1 780,862 - Sama_0628 0.25 +0.9 780,870 + Sama_0628 0.27 +2.1 780,871 - Sama_0628 0.27 +1.7 780,871 - Sama_0628 0.27 +3.2 780,871 - Sama_0628 0.27 +1.1 780,871 - Sama_0628 0.27 +1.7 780,871 - Sama_0628 0.27 +2.0 780,871 - Sama_0628 0.27 +2.1 780,955 + Sama_0628 0.46 +3.0 781,083 - Sama_0628 0.76 +2.1 781,185 + +0.7 781,185 + -1.3 781,185 + +0.7 781,185 + -0.3 781,185 + -0.9 781,185 + +1.1 781,186 - -0.5 781,186 - -1.1 781,186 - -0.2 781,186 - +1.7 781,186 - +1.0 781,186 - +0.0 781,242 - +0.6 781,268 - -0.4 781,351 + Sama_0629 0.13 +0.7 781,351 + Sama_0629 0.13 +1.0 781,352 - Sama_0629 0.13 +1.4 781,352 - Sama_0629 0.13 -0.3 781,362 + Sama_0629 0.14 +0.3 781,362 + Sama_0629 0.14 +0.4 781,362 + Sama_0629 0.14 +0.3 781,362 + Sama_0629 0.14 -0.0 781,362 + Sama_0629 0.14 -1.9 781,362 + Sama_0629 0.14 +0.1 781,363 - Sama_0629 0.14 -0.7 781,363 - Sama_0629 0.14 +0.7 781,363 - Sama_0629 0.14 +0.8 781,363 - Sama_0629 0.14 +0.3 781,363 - Sama_0629 0.14 +1.4 781,383 + Sama_0629 0.16 -0.3 781,383 + Sama_0629 0.16 +1.1 781,383 + Sama_0629 0.16 +0.8 781,383 + Sama_0629 0.16 +0.4 781,384 - Sama_0629 0.16 +0.7 781,384 - Sama_0629 0.16 +0.1 781,384 - Sama_0629 0.16 -0.5 781,384 - Sama_0629 0.16 +1.8 781,384 - Sama_0629 0.16 +0.1 781,384 - Sama_0629 0.16 -1.2 781,384 - Sama_0629 0.16 -0.9 781,412 + Sama_0629 0.18 -1.2 781,412 + Sama_0629 0.18 +0.4 781,412 + Sama_0629 0.18 +1.0 781,413 - Sama_0629 0.18 -0.5 781,413 - Sama_0629 0.18 +0.1 781,450 + Sama_0629 0.21 -0.2 781,450 + Sama_0629 0.21 +0.5 781,462 + Sama_0629 0.22 +1.2 781,463 - Sama_0629 0.23 +1.1 781,463 - Sama_0629 0.23 +0.7 781,463 - Sama_0629 0.23 +0.9 781,492 - Sama_0629 0.25 +1.2 781,513 + Sama_0629 0.27 +0.4 781,514 - Sama_0629 0.27 -1.2 781,523 + Sama_0629 0.27 +0.4 781,523 + Sama_0629 0.27 -0.6 781,524 - Sama_0629 0.28 -0.5 781,592 + Sama_0629 0.33 -0.1 781,592 + Sama_0629 0.33 -1.2 781,593 - Sama_0629 0.33 +1.2 781,624 + Sama_0629 0.36 +0.1 781,727 + Sama_0629 0.44 -0.9 781,727 + Sama_0629 0.44 -0.4 781,727 + Sama_0629 0.44 +0.2 781,727 + Sama_0629 0.44 -2.1 781,727 + Sama_0629 0.44 +0.9 781,727 + Sama_0629 0.44 -0.6 781,727 + Sama_0629 0.44 +0.0 781,727 + Sama_0629 0.44 +0.6 781,727 + Sama_0629 0.44 -0.8 781,727 + Sama_0629 0.44 -1.3 781,727 + Sama_0629 0.44 -0.3 781,727 + Sama_0629 0.44 +0.6 781,727 + Sama_0629 0.44 +0.7 781,727 + Sama_0629 0.44 +0.7 781,728 - Sama_0629 0.44 -0.0 781,728 - Sama_0629 0.44 -0.3 781,728 - Sama_0629 0.44 -1.9 781,728 - Sama_0629 0.44 -0.8 781,728 - Sama_0629 0.44 +0.6 781,728 - Sama_0629 0.44 +1.6 781,728 - Sama_0629 0.44 +0.9 781,728 - Sama_0629 0.44 -1.0 781,728 - Sama_0629 0.44 +2.3 781,728 - Sama_0629 0.44 +0.5 781,728 - Sama_0629 0.44 -0.5 781,761 + Sama_0629 0.47 +0.5 781,762 - Sama_0629 0.47 +0.2 781,762 - Sama_0629 0.47 -0.9 781,910 + Sama_0629 0.59 -0.9 781,910 + Sama_0629 0.59 -0.6 781,911 - Sama_0629 0.59 +1.0 781,911 - Sama_0629 0.59 -0.0 781,933 + Sama_0629 0.61 -0.7 781,934 - Sama_0629 0.61 +0.2 781,934 - Sama_0629 0.61 +0.1 781,934 - Sama_0629 0.61 -1.5 781,934 - Sama_0629 0.61 +0.4 781,971 + Sama_0629 0.64 +0.8 781,972 - Sama_0629 0.64 -1.9 781,972 - Sama_0629 0.64 -1.0 781,972 - Sama_0629 0.64 -0.9 781,972 - Sama_0629 0.64 +0.0 782,065 + Sama_0629 0.72 +0.4 782,065 + Sama_0629 0.72 -0.6 782,080 + Sama_0629 0.73 +0.7 782,081 - Sama_0629 0.73 +0.7 782,081 - Sama_0629 0.73 -1.3 782,081 - Sama_0629 0.73 +1.2 782,089 + Sama_0629 0.74 -1.3 782,090 - Sama_0629 0.74 -0.2 782,090 - Sama_0629 0.74 +0.7 782,090 - Sama_0629 0.74 +1.2 782,090 - Sama_0629 0.74 +0.7 782,090 - Sama_0629 0.74 +0.7 782,090 - Sama_0629 0.74 -1.6 782,139 + Sama_0629 0.78 -0.9 782,140 - Sama_0629 0.78 -1.5 782,140 - Sama_0629 0.78 +1.0 782,140 - Sama_0629 0.78 +0.1 782,249 - Sama_0629 0.87 -3.1 782,249 - Sama_0629 0.87 -0.7 782,323 - -1.3 782,346 + +0.4 782,385 + -0.0 782,402 - -1.6 782,420 + +0.2 782,450 + +0.4 782,451 - -2.5 782,705 + rumB Sama_0630 0.23 -0.5 782,764 + rumB Sama_0630 0.28 +0.7 782,765 - rumB Sama_0630 0.28 -0.9 782,803 + rumB Sama_0630 0.32 -0.4 782,803 + rumB Sama_0630 0.32 +0.2 782,804 - rumB Sama_0630 0.32 -0.1 782,804 - rumB Sama_0630 0.32 +0.7 782,804 - rumB Sama_0630 0.32 +1.6 782,804 - rumB Sama_0630 0.32 +1.1 782,804 - rumB Sama_0630 0.32 -0.5 782,804 - rumB Sama_0630 0.32 +0.0 782,810 - rumB Sama_0630 0.32 +0.4 782,810 - rumB Sama_0630 0.32 -0.5 782,845 + rumB Sama_0630 0.35 +1.2 782,845 + rumB Sama_0630 0.35 +0.5 782,846 - rumB Sama_0630 0.35 +1.2 782,846 - rumB Sama_0630 0.35 -0.9 782,846 - rumB Sama_0630 0.35 +0.1 782,932 + rumB Sama_0630 0.43 +0.5 782,932 + rumB Sama_0630 0.43 -1.2 782,932 + rumB Sama_0630 0.43 -1.4 782,932 + rumB Sama_0630 0.43 -2.1 782,933 - rumB Sama_0630 0.43 +1.6 783,027 + rumB Sama_0630 0.51 +0.1 783,027 + rumB Sama_0630 0.51 -1.5 783,027 + rumB Sama_0630 0.51 -0.9 783,099 + rumB Sama_0630 0.57 +0.9 783,100 - rumB Sama_0630 0.57 +0.3 783,102 - rumB Sama_0630 0.57 +1.1 783,132 + rumB Sama_0630 0.60 +0.3 783,132 + rumB Sama_0630 0.60 +0.9 783,132 + rumB Sama_0630 0.60 +1.0 783,133 - rumB Sama_0630 0.60 +0.2 783,133 - rumB Sama_0630 0.60 +0.2 783,133 - rumB Sama_0630 0.60 +2.1 783,169 - rumB Sama_0630 0.63 +0.5 783,280 + rumB Sama_0630 0.73 -0.9 783,357 + rumB Sama_0630 0.80 +0.9 783,357 + rumB Sama_0630 0.80 -2.3 783,357 + rumB Sama_0630 0.80 +0.7 783,357 + rumB Sama_0630 0.80 +0.6
Or see this region's nucleotide sequence