Experiment: NAG (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1133 and SO1134 are separated by 59 nucleotides SO1134 and SO1135 are separated by 57 nucleotides SO1135 and SO1136 are separated by 608 nucleotides
SO1133: SO1133 - ISSod1, transposase OrfA (NCBI ptt file), at 1,174,674 to 1,174,964
SO1133
SO1134: SO1134 - ISSod1, transposase OrfB (NCBI ptt file), at 1,175,024 to 1,175,833
SO1134
SO1135: SO1135 - hypothetical protein (NCBI ptt file), at 1,175,891 to 1,176,739
SO1135
SO1136: SO1136 - ATP-dependent RNA helicase, DEAD box family (NCBI ptt file), at 1,177,348 to 1,178,691
SO1136
Position (kb)
1175
1176
1177 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1175.897 kb on + strand at 1176.285 kb on + strand, within SO1135 at 1176.293 kb on - strand, within SO1135 at 1176.355 kb on + strand, within SO1135 at 1176.391 kb on - strand, within SO1135 at 1176.393 kb on - strand, within SO1135 at 1176.420 kb on - strand, within SO1135 at 1176.432 kb on + strand, within SO1135 at 1176.440 kb on - strand, within SO1135 at 1176.464 kb on - strand, within SO1135 at 1176.466 kb on + strand, within SO1135 at 1176.466 kb on + strand, within SO1135 at 1176.466 kb on + strand, within SO1135 at 1176.466 kb on + strand, within SO1135 at 1176.474 kb on - strand, within SO1135 at 1176.474 kb on - strand, within SO1135 at 1176.474 kb on - strand, within SO1135 at 1176.505 kb on + strand, within SO1135 at 1176.598 kb on + strand, within SO1135 at 1176.598 kb on + strand, within SO1135 at 1176.598 kb on + strand, within SO1135 at 1176.605 kb on + strand, within SO1135 at 1176.606 kb on - strand, within SO1135 at 1176.606 kb on - strand, within SO1135 at 1176.606 kb on - strand, within SO1135 at 1176.606 kb on - strand, within SO1135 at 1176.606 kb on - strand, within SO1135 at 1176.606 kb on - strand, within SO1135 at 1176.613 kb on + strand, within SO1135 at 1176.613 kb on - strand, within SO1135 at 1176.653 kb on + strand, within SO1135 at 1176.653 kb on + strand, within SO1135 at 1176.653 kb on + strand, within SO1135 at 1176.661 kb on - strand at 1176.680 kb on + strand at 1176.680 kb on + strand at 1176.688 kb on - strand at 1176.688 kb on - strand at 1176.688 kb on - strand at 1176.688 kb on - strand at 1176.734 kb on + strand at 1176.734 kb on + strand at 1176.749 kb on + strand at 1176.757 kb on - strand at 1176.790 kb on + strand at 1176.800 kb on - strand at 1176.805 kb on + strand at 1176.805 kb on + strand at 1176.805 kb on + strand at 1176.813 kb on - strand at 1176.814 kb on - strand at 1176.843 kb on - strand at 1176.851 kb on + strand at 1176.866 kb on + strand at 1176.877 kb on + strand at 1176.877 kb on + strand at 1176.890 kb on + strand at 1176.891 kb on + strand at 1176.896 kb on + strand at 1176.898 kb on + strand at 1176.898 kb on + strand at 1176.898 kb on + strand at 1176.906 kb on - strand at 1177.137 kb on - strand at 1177.141 kb on + strand at 1177.218 kb on + strand at 1177.218 kb on + strand at 1177.226 kb on - strand at 1177.273 kb on - strand at 1177.298 kb on + strand at 1177.299 kb on + strand at 1177.333 kb on - strand at 1177.340 kb on + strand at 1177.340 kb on + strand at 1177.363 kb on - strand at 1177.380 kb on + strand at 1177.380 kb on + strand at 1177.380 kb on + strand at 1177.388 kb on - strand at 1177.388 kb on - strand at 1177.388 kb on - strand at 1177.388 kb on - strand at 1177.388 kb on - strand at 1177.388 kb on - strand at 1177.439 kb on - strand at 1177.439 kb on - strand at 1177.439 kb on - strand at 1177.439 kb on - strand at 1177.459 kb on - strand at 1177.509 kb on + strand, within SO1136 at 1177.627 kb on + strand, within SO1136 at 1177.672 kb on - strand, within SO1136 at 1177.675 kb on - strand, within SO1136 at 1177.734 kb on - strand, within SO1136 at 1177.734 kb on - strand, within SO1136
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (N) remove 1,175,897 + -0.5 1,176,285 + SO1135 0.46 -0.0 1,176,293 - SO1135 0.47 +0.1 1,176,355 + SO1135 0.55 +1.0 1,176,391 - SO1135 0.59 +0.9 1,176,393 - SO1135 0.59 +0.5 1,176,420 - SO1135 0.62 +0.9 1,176,432 + SO1135 0.64 +0.3 1,176,440 - SO1135 0.65 +0.2 1,176,464 - SO1135 0.67 +0.2 1,176,466 + SO1135 0.68 +0.7 1,176,466 + SO1135 0.68 +0.4 1,176,466 + SO1135 0.68 -0.5 1,176,466 + SO1135 0.68 -1.1 1,176,474 - SO1135 0.69 +0.3 1,176,474 - SO1135 0.69 +1.4 1,176,474 - SO1135 0.69 +0.1 1,176,505 + SO1135 0.72 +2.7 1,176,598 + SO1135 0.83 +0.7 1,176,598 + SO1135 0.83 +1.1 1,176,598 + SO1135 0.83 +1.4 1,176,605 + SO1135 0.84 -1.8 1,176,606 - SO1135 0.84 +3.0 1,176,606 - SO1135 0.84 -0.0 1,176,606 - SO1135 0.84 +1.4 1,176,606 - SO1135 0.84 -2.8 1,176,606 - SO1135 0.84 -0.1 1,176,606 - SO1135 0.84 -0.2 1,176,613 + SO1135 0.85 +0.3 1,176,613 - SO1135 0.85 -1.4 1,176,653 + SO1135 0.90 +0.1 1,176,653 + SO1135 0.90 +1.0 1,176,653 + SO1135 0.90 -0.3 1,176,661 - -3.7 1,176,680 + +0.2 1,176,680 + -0.4 1,176,688 - +0.7 1,176,688 - -0.8 1,176,688 - -1.7 1,176,688 - -0.1 1,176,734 + -1.4 1,176,734 + +1.4 1,176,749 + -0.1 1,176,757 - +1.2 1,176,790 + -0.9 1,176,800 - -0.4 1,176,805 + -0.0 1,176,805 + -0.4 1,176,805 + +0.4 1,176,813 - -0.4 1,176,814 - +1.7 1,176,843 - +0.2 1,176,851 + -0.5 1,176,866 + +0.2 1,176,877 + +0.5 1,176,877 + +0.2 1,176,890 + +0.5 1,176,891 + +0.0 1,176,896 + +0.7 1,176,898 + +0.6 1,176,898 + -2.0 1,176,898 + +1.9 1,176,906 - +0.4 1,177,137 - +0.3 1,177,141 + -0.2 1,177,218 + +1.4 1,177,218 + -5.1 1,177,226 - -1.8 1,177,273 - +1.5 1,177,298 + -0.3 1,177,299 + +1.0 1,177,333 - +1.2 1,177,340 + -0.8 1,177,340 + -1.3 1,177,363 - +0.3 1,177,380 + +0.1 1,177,380 + -0.9 1,177,380 + +0.0 1,177,388 - +1.7 1,177,388 - -0.1 1,177,388 - -0.1 1,177,388 - +0.1 1,177,388 - -0.2 1,177,388 - +0.3 1,177,439 - -3.5 1,177,439 - -1.2 1,177,439 - -2.2 1,177,439 - -0.6 1,177,459 - -0.2 1,177,509 + SO1136 0.12 -1.0 1,177,627 + SO1136 0.21 +0.9 1,177,672 - SO1136 0.24 -0.2 1,177,675 - SO1136 0.24 +0.7 1,177,734 - SO1136 0.29 -0.8 1,177,734 - SO1136 0.29 +1.1
Or see this region's nucleotide sequence