Experiment: Glucose defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0186 and Psest_0187 are separated by 6 nucleotides Psest_0187 and Psest_0188 overlap by 4 nucleotides Psest_0188 and Psest_0189 are separated by 112 nucleotides Psest_0189 and Psest_0190 are separated by 2 nucleotides
Psest_0186: Psest_0186 - Protein-disulfide isomerase, at 181,742 to 182,371
_0186
Psest_0187: Psest_0187 - Metal-dependent hydrolase, at 182,378 to 183,247
_0187
Psest_0188: Psest_0188 - diguanylate cyclase (GGDEF) domain, at 183,244 to 185,142
_0188
Psest_0189: Psest_0189 - Negative regulator of beta-lactamase expression, at 185,255 to 186,043
_0189
Psest_0190: Psest_0190 - Uncharacterized conserved protein, at 186,046 to 186,450
_0190
Position (kb) 183
184
185
186 Strain fitness (log2 ratio) -2
-1
0
1
2 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.534 kb on + strand, within Psest_0187 at 182.534 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.619 kb on - strand, within Psest_0187 at 182.721 kb on - strand, within Psest_0187 at 182.933 kb on + strand, within Psest_0187 at 182.990 kb on - strand, within Psest_0187 at 183.277 kb on + strand at 183.278 kb on - strand at 183.341 kb on + strand at 183.488 kb on + strand, within Psest_0188 at 183.488 kb on + strand, within Psest_0188 at 183.601 kb on - strand, within Psest_0188 at 183.601 kb on - strand, within Psest_0188 at 184.069 kb on + strand, within Psest_0188 at 184.225 kb on + strand, within Psest_0188 at 184.226 kb on - strand, within Psest_0188 at 184.306 kb on + strand, within Psest_0188 at 184.307 kb on - strand, within Psest_0188 at 184.524 kb on + strand, within Psest_0188 at 184.529 kb on - strand, within Psest_0188 at 184.529 kb on - strand, within Psest_0188 at 185.178 kb on - strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.442 kb on - strand, within Psest_0189 at 185.442 kb on - strand, within Psest_0189 at 185.864 kb on + strand, within Psest_0189 at 185.961 kb on - strand, within Psest_0189 at 186.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glucose defined media remove 182,282 + Psest_0186 0.86 +2.1 182,282 + Psest_0186 0.86 -0.9 182,282 + Psest_0186 0.86 -2.0 182,282 + Psest_0186 0.86 +0.4 182,282 + Psest_0186 0.86 -0.3 182,534 + Psest_0187 0.18 +1.3 182,534 + Psest_0187 0.18 +0.7 182,618 + Psest_0187 0.28 +0.4 182,618 + Psest_0187 0.28 +1.7 182,618 + Psest_0187 0.28 +2.1 182,618 + Psest_0187 0.28 +0.5 182,619 - Psest_0187 0.28 -0.3 182,721 - Psest_0187 0.39 -0.1 182,933 + Psest_0187 0.64 +1.1 182,990 - Psest_0187 0.70 +0.7 183,277 + +1.5 183,278 - -0.2 183,341 + -0.1 183,488 + Psest_0188 0.13 -0.9 183,488 + Psest_0188 0.13 -1.2 183,601 - Psest_0188 0.19 +0.1 183,601 - Psest_0188 0.19 +0.4 184,069 + Psest_0188 0.43 +0.2 184,225 + Psest_0188 0.52 +0.1 184,226 - Psest_0188 0.52 +0.5 184,306 + Psest_0188 0.56 +0.5 184,307 - Psest_0188 0.56 +0.1 184,524 + Psest_0188 0.67 +0.0 184,529 - Psest_0188 0.68 -0.5 184,529 - Psest_0188 0.68 -0.5 185,178 - +1.1 185,332 + +0.5 185,332 + +0.7 185,332 + -0.1 185,332 + +0.0 185,332 + -0.2 185,332 + +0.9 185,333 - +0.9 185,333 - +2.1 185,333 - -0.5 185,333 - +1.1 185,333 - +1.1 185,333 - +0.1 185,378 + Psest_0189 0.16 -0.4 185,378 + Psest_0189 0.16 +1.1 185,378 + Psest_0189 0.16 +0.2 185,378 + Psest_0189 0.16 +0.1 185,378 + Psest_0189 0.16 -0.2 185,379 - Psest_0189 0.16 +1.1 185,379 - Psest_0189 0.16 +0.8 185,379 - Psest_0189 0.16 +0.0 185,379 - Psest_0189 0.16 +0.5 185,379 - Psest_0189 0.16 +0.1 185,379 - Psest_0189 0.16 +0.2 185,379 - Psest_0189 0.16 -0.4 185,379 - Psest_0189 0.16 +1.7 185,379 - Psest_0189 0.16 -0.1 185,442 - Psest_0189 0.24 -1.5 185,442 - Psest_0189 0.24 -0.5 185,864 + Psest_0189 0.77 +1.1 185,961 - Psest_0189 0.89 +0.5 186,051 - +0.1
Or see this region's nucleotide sequence