Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsN and SO3964 overlap by 4 nucleotides SO3964 and ptsO overlap by 14 nucleotides ptsO and mgtE-2 are separated by 196 nucleotides
SO3963: ptsN - nitrogen regulatory IIA protein (NCBI ptt file), at 4,103,897 to 4,104,340
ptsN
SO3964: SO3964 - conserved hypothetical protein (NCBI ptt file), at 4,104,337 to 4,105,191
SO3964
SO3965: ptsO - phosphocarrier protein NPR (NCBI ptt file), at 4,105,178 to 4,105,453
ptsO
SO3966: mgtE-2 - magnesium transporter (NCBI ptt file), at 4,105,650 to 4,107,014
mgtE-2
Position (kb) 4105
4106 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4104.278 kb on + strand, within ptsN at 4104.386 kb on + strand at 4104.394 kb on - strand at 4104.394 kb on - strand at 4104.465 kb on + strand, within SO3964 at 4104.473 kb on - strand, within SO3964 at 4104.473 kb on - strand, within SO3964 at 4104.574 kb on - strand, within SO3964 at 4104.942 kb on - strand, within SO3964 at 4105.286 kb on - strand, within ptsO at 4105.421 kb on + strand, within ptsO at 4105.443 kb on - strand at 4105.654 kb on - strand at 4105.680 kb on - strand at 4105.906 kb on + strand, within mgtE-2 at 4105.914 kb on - strand, within mgtE-2 at 4106.006 kb on + strand, within mgtE-2 at 4106.048 kb on + strand, within mgtE-2 at 4106.056 kb on - strand, within mgtE-2 at 4106.082 kb on - strand, within mgtE-2 at 4106.165 kb on - strand, within mgtE-2 at 4106.223 kb on - strand, within mgtE-2 at 4106.223 kb on - strand, within mgtE-2
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 4,104,278 + ptsN SO3963 0.86 -0.6 4,104,386 + -2.5 4,104,394 - -4.2 4,104,394 - -3.0 4,104,465 + SO3964 0.15 +0.4 4,104,473 - SO3964 0.16 -2.5 4,104,473 - SO3964 0.16 -3.1 4,104,574 - SO3964 0.28 -2.4 4,104,942 - SO3964 0.71 -4.0 4,105,286 - ptsO SO3965 0.39 -1.4 4,105,421 + ptsO SO3965 0.88 -1.3 4,105,443 - -1.9 4,105,654 - -2.8 4,105,680 - -0.4 4,105,906 + mgtE-2 SO3966 0.19 -2.4 4,105,914 - mgtE-2 SO3966 0.19 -1.0 4,106,006 + mgtE-2 SO3966 0.26 +0.2 4,106,048 + mgtE-2 SO3966 0.29 +0.1 4,106,056 - mgtE-2 SO3966 0.30 -1.0 4,106,082 - mgtE-2 SO3966 0.32 -0.9 4,106,165 - mgtE-2 SO3966 0.38 -0.7 4,106,223 - mgtE-2 SO3966 0.42 +1.4 4,106,223 - mgtE-2 SO3966 0.42 -1.4
Or see this region's nucleotide sequence